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TitleThe Eukaryotic CO-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization.
Journal, issue, pagesCell, Vol. 171, Issue 1, Page 148-162.e19, Year 2017
Publish dateSep 21, 2017
AuthorsElizabeth S Freeman Rosenzweig / Bin Xu / Luis Kuhn Cuellar / Antonio Martinez-Sanchez / Miroslava Schaffer / Mike Strauss / Heather N Cartwright / Pierre Ronceray / Jürgen M Plitzko / Friedrich Förster / Ned S Wingreen / Benjamin D Engel / Luke C M Mackinder / Martin C Jonikas /
PubMed AbstractApproximately 30%-40% of global CO fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is ...Approximately 30%-40% of global CO fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid. Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly. Our modeling predicts the existence of a "magic number" effect associated with special, highly stable heterocomplexes that influences phase separation in liquid-like organelles. This view of the pyrenoid matrix as a phase-separated compartment provides a paradigm for understanding its structure, biogenesis, and regulation. More broadly, our findings expand our understanding of the principles that govern the architecture and inheritance of liquid-like organelles.
External linksCell / PubMed:28938114 / PubMed Central
MethodsEM (tomography) / EM (subtomogram averaging)
Resolution16.5 Å
Structure data

EMDB-12749:
In situ cryo-electron tomogram of a pyrenoid inside a Chlamydomonas reinhardtii cell
Method: EM (tomography)

EMDB-3694:
In situ subtomogram average of Rubisco within the Chlamydomonas pyrenoid
Method: EM (subtomogram averaging) / Resolution: 16.5 Å

Source
  • Chlamydomonas reinhardtii (plant)

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