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Structure paper

TitleStructure of the complete elongation complex of RNA polymerase II with basal factors.
Journal, issue, pagesScience, Vol. 357, Issue 6354, Page 921-924, Year 2017
Publish dateSep 1, 2017
AuthorsHaruhiko Ehara / Takeshi Yokoyama / Hideki Shigematsu / Shigeyuki Yokoyama / Mikako Shirouzu / Shun-Ichi Sekine /
PubMed AbstractIn the early stage of transcription, eukaryotic RNA polymerase II (Pol II) exchanges initiation factors with elongation factors to form an elongation complex for processive transcription. Here we ...In the early stage of transcription, eukaryotic RNA polymerase II (Pol II) exchanges initiation factors with elongation factors to form an elongation complex for processive transcription. Here we report the structure of the Pol II elongation complex bound with the basal elongation factors Spt4/5, Elf1, and TFIIS. Spt4/5 (the Spt4/Spt5 complex) and Elf1 modify a wide area of the Pol II surface. Elf1 bridges the Pol II central cleft, completing a "DNA entry tunnel" for downstream DNA. Spt4 and the Spt5 NGN and KOW1 domains encircle the upstream DNA, constituting a "DNA exit tunnel." The Spt5 KOW4 and KOW5 domains augment the "RNA exit tunnel," directing the exiting nascent RNA. Thus, the elongation complex establishes a completely different transcription and regulation platform from that of the initiation complexes.
External linksScience / PubMed:28775211
MethodsEM (single particle) / X-ray diffraction
Resolution3 - 3.83 Å
Structure data

6747

5xon

EMDB-6747, PDB-5xon:
RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS
Method: EM (single particle) / Resolution: 3.83 Å

PDB-5xog:
RNA Polymerase II elongation complex bound with Spt5 KOW5 and Elf1
Method: X-RAY DIFFRACTION / Resolution: 3 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-APC:
DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER / AMP-CPP, energy-carrying molecule analogue*YM

Source
  • komagataella pastoris (fungus)
  • komagataella phaffii (strain gs115 / atcc 20864) (fungus)
  • synthetic construct (others)
  • komagataella phaffii (fungus)
  • komagataella phaffii (strain atcc 76273 / cbs 7435 / cect 11047 / nrrl y-11430 / wegner 21-1) (fungus)
KeywordsTRANSCRIPTION / complex / TRANSCRIPTION/RNA / TRANSCRIPTION-RNA complex

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