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Structure paper

TitleStructure of the complete elongation complex of RNA polymerase II with basal factors.
Journal, issue, pagesScience, Vol. 357, Issue 6354, Page 921-924, Year 2017
Publish dateSep 1, 2017
AuthorsHaruhiko Ehara / Takeshi Yokoyama / Hideki Shigematsu / Shigeyuki Yokoyama / Mikako Shirouzu / Shun-Ichi Sekine
External linksPubMed:28775211 / Publisher's page
MethodsEM (single particle) / X-ray diffraction
Resolution3 - 3.83 A
Structure data

EMDB-6747:
RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS

PDB-5xog:
RNA Polymerase II elongation complex bound with Spt5 KOW5 and Elf1

PDB-5xon:
RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS

Chemicals

ChemComp-ZN:
ZINC ION

ChemComp-MG:
MAGNESIUM ION

ChemComp-APC:
DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER

SourceKomagataella pastoris / fungus
Komagataella phaffii (strain gs115 / atcc 20864) / fungus
Komagataella phaffii / fungus
Komagataella phaffii (strain atcc 76273 / cbs 7435 / cect 11047 / nrrl y-11430 / wegner 21-1) / fungus
Synthetic construct
KeywordsTRANSCRIPTION / transcription / complex / TRANSCRIPTION/RNA / TRANSCRIPTION-RNA complex

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Oct 4, 2017. Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

  • Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
  • Joachim Frank (Columbia University, New York, USA) is a pioneer of single particle reconstruction, which is the most used reconstruction method for 3DEM structures in EMDB and EM entries in PDB. And also, he is a develper of Spider, which is one of the most famous software in this field, and is used for some EM Navigor data (e.g. map projection/slice images).
  • Richard Henderson (MRC Laboratory of Molecular Biology, Cambridge, UK) was determined the first biomolecule structure by EM. The first EM entry in PDB, PDB-1brd is determinedby him.

External links: The 2017 Nobel Prize in Chemistry - Press Release

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Major update of PDB

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External links: wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

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EMN Papers

Database of articles cited by 3DEM data entries

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