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- EMDB-4131: Structure of the mammalian ribosomal termination complex with eRF... -

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Basic information

Entry
Database: EMDB / ID: EMD-4131
TitleStructure of the mammalian ribosomal termination complex with eRF1 and eRF3.
Map dataPostprocess, sharpened map.
Sample
  • Complex: Affinity-purified 80S ribosome-nascent chain complex reconstituted with eRF1 and eRF3.
    • Protein or peptide: x 80 types
    • RNA: x 7 types
  • Ligand: x 3 types
Function / homology
Function and homology information


translation termination factor activity / cytoplasmic translational termination / translation release factor complex / translation release factor activity / regulation of translational termination / translation release factor activity, codon specific / protein methylation / sequence-specific mRNA binding / aminoacyl-tRNA hydrolase activity / regulation of G1 to G0 transition ...translation termination factor activity / cytoplasmic translational termination / translation release factor complex / translation release factor activity / regulation of translational termination / translation release factor activity, codon specific / protein methylation / sequence-specific mRNA binding / aminoacyl-tRNA hydrolase activity / regulation of G1 to G0 transition / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / optic nerve development / retinal ganglion cell axon guidance / mammalian oogenesis stage / G1 to G0 transition / activation-induced cell death of T cells / Protein hydroxylation / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / phagocytic cup / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / 90S preribosome / TOR signaling / T cell proliferation involved in immune response / erythrocyte development / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / ribosomal small subunit export from nucleus / translation regulator activity / translational termination / rough endoplasmic reticulum / gastrulation / MDM2/MDM4 family protein binding / maturation of LSU-rRNA / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / cytosolic ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / rescue of stalled ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / cellular response to leukemia inhibitory factor / positive regulation of translation / small-subunit processome / protein kinase C binding / positive regulation of apoptotic signaling pathway / positive regulation of protein-containing complex assembly / placenta development / cellular response to gamma radiation / mRNA 5'-UTR binding / transcription coactivator binding / Regulation of expression of SLITs and ROBOs / spindle / cytoplasmic ribonucleoprotein granule / modification-dependent protein catabolic process / G1/S transition of mitotic cell cycle / protein tag activity / rRNA processing / ribosomal small subunit biogenesis / antimicrobial humoral immune response mediated by antimicrobial peptide / rhythmic process / positive regulation of canonical Wnt signaling pathway / small ribosomal subunit rRNA binding / ribosome binding / glucose homeostasis / regulation of translation / ribosomal small subunit assembly / retina development in camera-type eye / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / T cell differentiation in thymus / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cell body / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / cytoplasmic translation / perikaryon / cytosolic large ribosomal subunit / defense response to Gram-negative bacterium / killing of cells of another organism / tRNA binding / mitochondrial inner membrane / postsynaptic density / cell differentiation / protein stabilization / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / positive regulation of apoptotic process
Similarity search - Function
Peptide chain release factor eRF1/aRF1 / eRF1, domain 1 / eRF1 domain 2 / eRF1 domain 2 / eRF1 domain 1/Pelota-like / eRF1 domain 3 / eRF1, domain 2 superfamily / eRF1 domain 1 / eRF1 domain 3 / eRF1_1 ...Peptide chain release factor eRF1/aRF1 / eRF1, domain 1 / eRF1 domain 2 / eRF1 domain 2 / eRF1 domain 1/Pelota-like / eRF1 domain 3 / eRF1, domain 2 superfamily / eRF1 domain 1 / eRF1 domain 3 / eRF1_1 / GTP-eEF1A C-terminal domain-like / : / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ubiquitin-like protein FUBI / Ribosomal protein L30e / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / metallochaperone-like domain / TRASH domain / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L10e / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S30 / : / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S7e signature. / : / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / : / Ribosomal protein S19e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein L30e signature 1. / Ribosomal protein L6e signature. / Ribosomal protein S3Ae signature. / Ribosomal_S19e / Ribosomal protein S27e signature. / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein L30e signature 2. / Ribosomal protein S19A/S15e / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L30e, conserved site / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal protein S6, eukaryotic / Ribosomal protein L34Ae / Ribosomal protein L34e / 40S ribosomal protein S1/3, eukaryotes
Similarity search - Domain/homology
Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL29 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 ...Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL29 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein eL8 / Small ribosomal subunit protein eS7 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein eL15 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL14 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS8 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL36 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL27 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL42 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL28 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein eL34 / Eukaryotic peptide chain release factor GTP-binding subunit ERF3A / Eukaryotic peptide chain release factor subunit 1 / Large ribosomal subunit protein eL37
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit) / Rabbit (rabbit) / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.65 Å
AuthorsShao S / Murray J / Brown A / Taunton J / Ramakrishnan V / Hegde RS
Funding support United Kingdom, 3 items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)MC_UP_A022_1007 United Kingdom
Wellcome TrustWT096570 United Kingdom
Medical Research Council (United Kingdom)MC_U105184332 United Kingdom
Citation
Journal: Cell / Year: 2016
Title: Decoding Mammalian Ribosome-mRNA States by Translational GTPase Complexes.
Authors: Sichen Shao / Jason Murray / Alan Brown / Jack Taunton / V Ramakrishnan / Ramanujan S Hegde /
Abstract: In eukaryotes, accurate protein synthesis relies on a family of translational GTPases that pair with specific decoding factors to decipher the mRNA code on ribosomes. We present structures of the ...In eukaryotes, accurate protein synthesis relies on a family of translational GTPases that pair with specific decoding factors to decipher the mRNA code on ribosomes. We present structures of the mammalian ribosome engaged with decoding factor⋅GTPase complexes representing intermediates of translation elongation (aminoacyl-tRNA⋅eEF1A), termination (eRF1⋅eRF3), and ribosome rescue (Pelota⋅Hbs1l). Comparative analyses reveal that each decoding factor exploits the plasticity of the ribosomal decoding center to differentially remodel ribosomal proteins and rRNA. This leads to varying degrees of large-scale ribosome movements and implies distinct mechanisms for communicating information from the decoding center to each GTPase. Additional structural snapshots of the translation termination pathway reveal the conformational changes that choreograph the accommodation of decoding factors into the peptidyl transferase center. Our results provide a structural framework for how different states of the mammalian ribosome are selectively recognized by the appropriate decoding factor⋅GTPase complex to ensure translational fidelity.
#1: Journal: To Be Published
Title: Decoding mammalian ribosome-mRNA states by translational GTPase complexes
Authors: Shao S / Murray J / Brown A / Taunton J / Ramakrishnan V / Hegde RS
History
DepositionOct 2, 2016-
Header (metadata) releaseNov 23, 2016-
Map releaseNov 30, 2016-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.1
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.1
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5lzt
  • Surface level: 0.1
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4131.png

Downloads & links

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Map

FileDownload / File: emd_4131.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPostprocess, sharpened map.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.34 Å/pix.
x 420 pix.
= 562.8 Å		1.34 Å/pix.
x 420 pix.
= 562.8 Å		1.34 Å/pix.
x 420 pix.
= 562.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.34 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1 / Movie #1: 0.1
Minimum - Maximum-0.49338907 - 0.8001313
Average (Standard dev.)0.001357231 (±0.022760976)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 562.8 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.341.341.34
M x/y/z420420420
origin x/y/z0.0000.0000.000
length x/y/z562.800562.800562.800
α/β/γ90.00090.00090.000
start NX/NY/NZ-165-165-165
NX/NY/NZ330330330
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS420420420
D min/max/mean-0.4930.8000.001

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Supplemental data

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Half map: Half map 1.

Fileemd_4131_half_map_1.map
AnnotationHalf map 1.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map 2.

Fileemd_4131_half_map_2.map
AnnotationHalf map 2.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Affinity-purified 80S ribosome-nascent chain complex reconstitute...

EntireName: Affinity-purified 80S ribosome-nascent chain complex reconstituted with eRF1 and eRF3.
Components
  • Complex: Affinity-purified 80S ribosome-nascent chain complex reconstituted with eRF1 and eRF3.
    • Protein or peptide: uL2
    • Protein or peptide: uL3
    • Protein or peptide: uL4
    • Protein or peptide: uL18
    • Protein or peptide: eL6
    • Protein or peptide: uL30
    • Protein or peptide: eL8
    • Protein or peptide: uL6
    • Protein or peptide: uL16
    • Protein or peptide: uL5
    • Protein or peptide: eL13
    • Protein or peptide: eL14
    • Protein or peptide: eL15
    • Protein or peptide: uL13
    • Protein or peptide: uL22
    • Protein or peptide: eL18
    • Protein or peptide: eL19
    • Protein or peptide: eL20
    • Protein or peptide: eL21
    • Protein or peptide: eL22
    • Protein or peptide: uL14
    • Protein or peptide: eL24
    • Protein or peptide: uL23
    • Protein or peptide: uL24
    • Protein or peptide: eL27
    • Protein or peptide: uL15
    • Protein or peptide: eL29
    • Protein or peptide: eL30
    • Protein or peptide: eL31
    • Protein or peptide: eL32
    • Protein or peptide: eL33
    • Protein or peptide: eL34
    • Protein or peptide: uL29
    • Protein or peptide: eL36
    • Protein or peptide: eL37
    • Protein or peptide: eL38
    • Protein or peptide: eL39
    • Protein or peptide: eL40
    • Protein or peptide: eL41
    • Protein or peptide: eL42
    • Protein or peptide: eL43
    • Protein or peptide: eL28
    • Protein or peptide: uL10
    • Protein or peptide: uL11
    • Protein or peptide: Nascent chain
    • RNA: P-site tRNA
    • RNA: E-site tRNA
    • RNA: 28S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • RNA: 18S ribosomal RNA
    • Protein or peptide: uS2
    • Protein or peptide: eS1
    • Protein or peptide: uS5
    • Protein or peptide: uS3
    • Protein or peptide: eS4
    • Protein or peptide: uS7
    • Protein or peptide: eS6
    • Protein or peptide: eS7
    • Protein or peptide: eS8
    • Protein or peptide: uS4
    • Protein or peptide: eS10
    • Protein or peptide: uS17
    • Protein or peptide: eS12
    • Protein or peptide: uS15
    • Protein or peptide: uS11
    • Protein or peptide: uS19
    • Protein or peptide: uS9
    • Protein or peptide: eS17
    • Protein or peptide: uS13
    • Protein or peptide: eS19
    • Protein or peptide: uS10
    • Protein or peptide: eS21
    • Protein or peptide: uS8
    • Protein or peptide: uS12
    • Protein or peptide: eS24
    • Protein or peptide: eS25
    • Protein or peptide: eS26
    • Protein or peptide: eS27
    • Protein or peptide: eS28
    • Protein or peptide: uS14
    • Protein or peptide: eS30
    • Protein or peptide: eS31
    • Protein or peptide: RACK1
    • RNA: mRNA (UGA stop codon)
    • Protein or peptide: eRF1
    • Protein or peptide: eRF3a
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

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Supramolecule #1: Affinity-purified 80S ribosome-nascent chain complex reconstitute...

SupramoleculeName: Affinity-purified 80S ribosome-nascent chain complex reconstituted with eRF1 and eRF3.
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#87
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 3.3 MDa

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Macromolecule #1: uL2

MacromoleculeName: uL2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 28.088863 KDa
SequenceString: MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV ...String:
MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGNHQH IGKPSTIRRD APAGRKVGLI AA RRTGRLR GTKTVQEKEN

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Macromolecule #2: uL3

MacromoleculeName: uL3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 46.107977 KDa
SequenceString: MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM ...String:
MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVS QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKTHRG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINPL GGF VHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTVEEKK AFMGPLKKDR IAKE EGA

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Macromolecule #3: uL4

MacromoleculeName: uL4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 47.727559 KDa
SequenceString: MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV ...String:
MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC VIYNEDNGIV KAFRNIPGIT LLNVTKLNIL KL APGGHVG RFCIWTESAF RKLDDLYGTW RKAASLKSNY NLPMHKMLNT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVERAAA ALAAKSDPKE APAKKKPVVG KKVKKPRAVG IKQKKKPVVG RKAA AAKKP AADKKAADKR AGPEDKKPAA

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Macromolecule #4: uL18

MacromoleculeName: uL18 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 34.481828 KDa
SequenceString: MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD ...String:
MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMMEE MY KKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

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Macromolecule #5: eL6

MacromoleculeName: eL6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 33.028336 KDa
SequenceString: MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL ...String:
MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKKLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

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Macromolecule #6: uL30

MacromoleculeName: uL30 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.201836 KDa
SequenceString: MEGAEEKKKV PAVPETLKKK RRNFAELKIK RLRKKFAQKM LRKARRKLIY EKAKHYHKEY RQMYRTEIRM ARMARKAGNF YVPAEPKLA FVIRIRGING VSPKVRKVLQ LLRLRQIFNG TFVKLNKASI NMLRIVEPYI AWGYPNLKSV NELIYKRGYG K INKKRIAL ...String:
MEGAEEKKKV PAVPETLKKK RRNFAELKIK RLRKKFAQKM LRKARRKLIY EKAKHYHKEY RQMYRTEIRM ARMARKAGNF YVPAEPKLA FVIRIRGING VSPKVRKVLQ LLRLRQIFNG TFVKLNKASI NMLRIVEPYI AWGYPNLKSV NELIYKRGYG K INKKRIAL TDNTLIARSL GKYNIICMED LIHEIYTVGK HFKEANNFLW PFKLSSPRGG MKKKTTHFVE GGDAGNREDQ IN RLIRRMN

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Macromolecule #7: eL8

MacromoleculeName: eL8 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 36.221516 KDa
SequenceString: MSSYRLGYCM KEERHNLVLC LWSQSPGILN SKCLWPFTNI HLLVGALPRE GAGGAWGGGR SEQLPTCSTT HHDFTWDKKV VNPLFEKRP KNFGIGQDIQ PKRDLTRFVK WPRYIRLQRQ RAILYKRLKV PPAINQFTQV LDRQTATQLL KLAHKYRPET K QEKKQRLL ...String:
MSSYRLGYCM KEERHNLVLC LWSQSPGILN SKCLWPFTNI HLLVGALPRE GAGGAWGGGR SEQLPTCSTT HHDFTWDKKV VNPLFEKRP KNFGIGQDIQ PKRDLTRFVK WPRYIRLQRQ RAILYKRLKV PPAINQFTQV LDRQTATQLL KLAHKYRPET K QEKKQRLL ARAEKKAAGK GDVPTKRPPV LRAGVNTVTT LVENKKAQLV VIAHDVDPIE LVVFLPALCR KMGVPYCILK GK ARLGRLV HRKTCTTVAF TQVNSEDKGA LAKLVEAIRT NYNDRYDEIR RHWGGNVLGP KSVARIAKLE KAKAKELATK LG

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Macromolecule #8: uL6

MacromoleculeName: uL6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.871418 KDa
SequenceString: MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK ...String:
MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

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Macromolecule #9: uL16

MacromoleculeName: uL16 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.643057 KDa
SequenceString: MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS ...String:
MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

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Macromolecule #10: uL5

MacromoleculeName: uL5 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 20.288465 KDa
SequenceString:
MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

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Macromolecule #11: eL13

MacromoleculeName: eL13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.331723 KDa
SequenceString: MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ...String:
MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

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Macromolecule #12: eL14

MacromoleculeName: eL14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.870549 KDa
SequenceString: MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG ...String:
MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG KKAAAQKAPA QKAPAQKAAG QKAAQPPKAQ KGQKPPAQKA PAPKASGKKA

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Macromolecule #13: eL15

MacromoleculeName: eL15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.207285 KDa
SequenceString: MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG ...String:
MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

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Macromolecule #14: uL13

MacromoleculeName: uL13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.533299 KDa
SequenceString: MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY ...String:
MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK ISKFTDVLKT HGLLV

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Macromolecule #15: uL22

MacromoleculeName: uL22 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.444221 KDa
SequenceString:
MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLKKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

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Macromolecule #16: eL18

MacromoleculeName: eL18 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.699688 KDa
SequenceString:
MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTVTDD VRVQEVPKLK VCALRVTSRA RSRILKAGGK ILTFDQLALD SPKGRGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

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Macromolecule #17: eL19

MacromoleculeName: eL19 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.535281 KDa
SequenceString: MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ...String:
MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

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Macromolecule #18: eL20

MacromoleculeName: eL20 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 20.827561 KDa
SequenceString:
MKASGTLREY KVVGRCLPTP KCRTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

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Macromolecule #19: eL21

MacromoleculeName: eL21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.609988 KDa
SequenceString:
MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

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Macromolecule #20: eL22

MacromoleculeName: eL22 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.813015 KDa
SequenceString:
MAPVKKLVAK GGKKKKQLLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VSLERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANTKES YELRYFQINQ DEEEEEEED

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Macromolecule #21: uL14

MacromoleculeName: uL14 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.892505 KDa
SequenceString:
MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

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Macromolecule #22: eL24

MacromoleculeName: eL24 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.825111 KDa
SequenceString:
MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

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Macromolecule #23: uL23

MacromoleculeName: uL23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.768246 KDa
SequenceString:
MAPKAKKEAP APPKVEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

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Macromolecule #24: uL24

MacromoleculeName: uL24 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.303363 KDa
SequenceString:
MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

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Macromolecule #25: eL27

MacromoleculeName: eL27 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.835831 KDa
SequenceString:
MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

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Macromolecule #26: uL15

MacromoleculeName: uL15 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.620561 KDa
SequenceString:
MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGMHH HRINFDKYHP GYFGKVGMRH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKG VGGACVLVA

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Macromolecule #27: eL29

MacromoleculeName: eL29 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 26.708707 KDa
SequenceString: MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE ...String:
MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE TKPKAQAQAK PKAQAQGKPK AQAQGKPKAQ AQAKPKAQAQ AKPKAQAQTK PKAQATPAAP VPAQAPPKGA QP PAKAP

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Macromolecule #28: eL30

MacromoleculeName: eL30 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.807065 KDa
SequenceString:
MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQSLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLSIIDP GDSDIIRSMP EQTGEK

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Macromolecule #29: eL31

MacromoleculeName: eL31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.494938 KDa
SequenceString:
MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

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Macromolecule #30: eL32

MacromoleculeName: eL32 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.898932 KDa
SequenceString:
MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

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Macromolecule #31: eL33

MacromoleculeName: eL33 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.580809 KDa
SequenceString:
MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

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Macromolecule #32: eL34

MacromoleculeName: eL34 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.326074 KDa
SequenceString:
MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKAK

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Macromolecule #33: uL29

MacromoleculeName: uL29 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.566599 KDa
SequenceString:
MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ESLKTKKQQR KERLYPLRKY AVKA

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Macromolecule #34: eL36

MacromoleculeName: eL36 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.263834 KDa
SequenceString:
MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSSVLAAMRK AAAKKD

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Macromolecule #35: eL37

MacromoleculeName: eL37 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 11.111032 KDa
SequenceString:
MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

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Macromolecule #36: eL38

MacromoleculeName: eL38 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 8.238948 KDa
SequenceString:
MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

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Macromolecule #37: eL39

MacromoleculeName: eL39 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 6.455775 KDa
SequenceString:
MSSHKTFRIK RFLAKKQKQN RPIPQWIWMK TGNKIRYNSK RRHWRRTKLG L

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Macromolecule #38: eL40

MacromoleculeName: eL40 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 11.69979 KDa
SequenceString:
MGDPESGGCI PPDQQRLIFA GKQLEDGRTL SDYNIQKEST LHLVLRLRGG IIEPSLRQLA QKYNCDKMIC RKCYARLHPR AVNCRKKKC GHTNNLRPKK KVK

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Macromolecule #39: eL41

MacromoleculeName: eL41 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 3.473451 KDa
SequenceString:
MRAKWRKKRM RRLKRKRRKM RQRSK

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Macromolecule #40: eL42

MacromoleculeName: eL42 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.476973 KDa
SequenceString:
MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QTKPIFRKKA KTTKKIVLRL ECVEPNCRSK RMLAIKRCK HFELGGDKKR KGQVIQF

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Macromolecule #41: eL43

MacromoleculeName: eL43 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 10.29935 KDa
SequenceString:
MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

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Macromolecule #42: eL28

MacromoleculeName: eL28 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.783614 KDa
SequenceString:
MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYHPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

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Macromolecule #43: uL10

MacromoleculeName: uL10 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 34.380504 KDa
SequenceString: MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ...String:
MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ASEATLLNML NISPFSFGLI IQQVFDNGSI YNPEVLDITE DTLHSRFLEG VRNVASVCLQ IGYPTVASVP HS IINGYKR VLALSVETEY TFPLAEKVKA FLADPSAFVA AAPVAAASTA APAAAAAAPA KVEAKEESEE SDEDMGFGLF D

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Macromolecule #44: uL11

MacromoleculeName: uL11 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.847619 KDa
SequenceString:
MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

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Macromolecule #45: Nascent chain

MacromoleculeName: Nascent chain / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 1.788032 KDa
SequenceString:
GMWRFYTEDS PGLKV

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Macromolecule #52: uS2

MacromoleculeName: uS2 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 32.958016 KDa
SequenceString: MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQTAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA ...String:
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQTAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGTTTEWS

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Macromolecule #53: eS1

MacromoleculeName: eS1 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 30.002061 KDa
SequenceString: MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM ...String:
MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

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Macromolecule #54: uS5

MacromoleculeName: uS5 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 31.327395 KDa
SequenceString: MADDAGAAGG PGDPGGPGIG GRGGFRGGFG SGVRGRGRGR GRGRGRGRGA RGGKAEDKEW LPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG ...String:
MADDAGAAGG PGDPGGPGIG GRGGFRGGFG SGVRGRGRGR GRGRGRGRGA RGGKAEDKEW LPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

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Macromolecule #55: uS3

MacromoleculeName: uS3 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 26.715344 KDa
SequenceString: MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV ...String:
MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPSGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

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Macromolecule #56: eS4

MacromoleculeName: eS4 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.654869 KDa
SequenceString: MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ...String:
MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

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Macromolecule #57: uS7

MacromoleculeName: uS7 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 22.913453 KDa
SequenceString: MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT ...String:
MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

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Macromolecule #58: eS6

MacromoleculeName: eS6 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 28.751906 KDa
SequenceString: MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK ...String:
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

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Macromolecule #59: eS7

MacromoleculeName: eS7 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 22.168914 KDa
SequenceString: MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH ...String:
MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

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Macromolecule #60: eS8

MacromoleculeName: eS8 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.263387 KDa
SequenceString: MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ ...String:
MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

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Macromolecule #61: uS4

MacromoleculeName: uS4 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 22.641564 KDa
SequenceString: MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG ...String:
MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

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Macromolecule #62: eS10

MacromoleculeName: eS10 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.933846 KDa
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

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Macromolecule #63: uS17

MacromoleculeName: uS17 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.468826 KDa
SequenceString:
MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

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Macromolecule #64: eS12

MacromoleculeName: eS12 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.538987 KDa
SequenceString:
MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

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Macromolecule #65: uS15

MacromoleculeName: uS15 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.259389 KDa
SequenceString:
MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

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Macromolecule #66: uS11

MacromoleculeName: uS11 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.133984 KDa
SequenceString:
MKARALSGSG VRRRRAAMAP RKGKEKKEEQ VISLGPQVAE GENVFGVCHI FASFNDTFVH VTDLSGKETI CRVTGGMKVK ADRDESSPY AAMLAAQDVA QRCKELGITA LHIKLRATGG NRTKTPGPGA QSALRALARS GMKIGRIEDV TPIPSDSTRR K GGRRGRRL

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Macromolecule #67: uS19

MacromoleculeName: uS19 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.049182 KDa
SequenceString:
MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

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Macromolecule #68: uS9

MacromoleculeName: uS9 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.477377 KDa
SequenceString:
MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK ERFAGVDIRV RVKGGGHVAQ IYAIRQSIS KALVAYYQKY VDEASKKEIK DILIQYDRTL LVADPRRCES KKFGGPGARA RYQKSYR

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Macromolecule #69: eS17

MacromoleculeName: eS17 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.552119 KDa
SequenceString:
MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

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Macromolecule #70: uS13

MacromoleculeName: uS13 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.759777 KDa
SequenceString:
MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

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Macromolecule #71: eS19

MacromoleculeName: eS19 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.10664 KDa
SequenceString:
MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AAKKKH

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Macromolecule #72: uS10

MacromoleculeName: uS10 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.398763 KDa
SequenceString:
MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

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Macromolecule #73: eS21

MacromoleculeName: eS21 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 9.124389 KDa
SequenceString:
MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

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Macromolecule #74: uS8

MacromoleculeName: uS8 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.865555 KDa
SequenceString:
MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

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Macromolecule #75: uS12

MacromoleculeName: uS12 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.844666 KDa
SequenceString:
MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

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Macromolecule #76: eS24

MacromoleculeName: eS24 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.107924 KDa
SequenceString:
MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK K

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Macromolecule #77: eS25

MacromoleculeName: eS25 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.776224 KDa
SequenceString:
MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQ ELLSKGLIKL VSKHRAQVIY TRNTKGGDAP AAGEDA

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Macromolecule #78: eS26

MacromoleculeName: eS26 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.047532 KDa
SequenceString:
MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

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Macromolecule #79: eS27

MacromoleculeName: eS27 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 9.480186 KDa
SequenceString:
MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

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Macromolecule #80: eS28

MacromoleculeName: eS28 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 7.855052 KDa
SequenceString:
MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

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Macromolecule #81: uS14

MacromoleculeName: uS14 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 6.690821 KDa
SequenceString:
MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

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Macromolecule #82: eS30

MacromoleculeName: eS30 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.498884 KDa
SequenceString:
MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

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Macromolecule #83: eS31

MacromoleculeName: eS31 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.004041 KDa
SequenceString:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

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Macromolecule #84: RACK1

MacromoleculeName: RACK1 / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 35.115652 KDa
SequenceString: MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC ...String:
MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

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Macromolecule #86: eRF1

MacromoleculeName: eRF1 / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 51.639512 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MRGSHHHHHH GMASENLYFQ GSMADDPSAA DRNVEIWKIK KLIKSLEAAR GNGTSMISLI IPPKDQISRV AKMLADEFGT ASNIKSRVN RLSVLGAITS VQQRLKLYNK VPPNGLVVYC GTIVTEEGKE KKVNIDFEPF KPINTSLYLC DNKFHTEALT A LLSDDSKF ...String:
MRGSHHHHHH GMASENLYFQ GSMADDPSAA DRNVEIWKIK KLIKSLEAAR GNGTSMISLI IPPKDQISRV AKMLADEFGT ASNIKSRVN RLSVLGAITS VQQRLKLYNK VPPNGLVVYC GTIVTEEGKE KKVNIDFEPF KPINTSLYLC DNKFHTEALT A LLSDDSKF GFIVIDGSGA LFGTLQGNTR EVLHKFTVDL PKKHGRGGQS ALRFARLRME KRHNYVRKVA ETAVQLFISG DK VNVAGLV LAGSADFKTE LSQSDMFDQR LQSKVLKLVD ISYGGENGFN QAIELSTEVL SNVKFIQEKK LIGRYFDEIS QDT GKYCFG VEDTLKALEM GAVEILIVYE NLDIMRYVLH CQGTEEEKIL YLTPEQEKDK SHFTDKETGQ EHELIESMPL LEWF ANNYK KFGATLEIVT DKSQEGSQFV KGFGGIGGIL RYRVDFQGME YQGGDDEFFD LDDY

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Macromolecule #87: eRF3a

MacromoleculeName: eRF3a / type: protein_or_peptide / ID: 87 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 68.756469 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MDPGSGGGGG GGGGGGSSSG SSSSDSAPDC WDQADMEAPG PGPCGGGGSL AAAAEAQREN LSAAFSRQLN VNAKPFVPNV HAAEFVPSF LRGPAAPPPP AGGAANNHGA GSGAGGRAAP VESSQEEQSL CEGSNSAVSM ELSEPIVENG ETEMSPEESW E HKEEISEA ...String:
MDPGSGGGGG GGGGGGSSSG SSSSDSAPDC WDQADMEAPG PGPCGGGGSL AAAAEAQREN LSAAFSRQLN VNAKPFVPNV HAAEFVPSF LRGPAAPPPP AGGAANNHGA GSGAGGRAAP VESSQEEQSL CEGSNSAVSM ELSEPIVENG ETEMSPEESW E HKEEISEA EPGGGSLGDG RPPEESAHEM MEEEEEIPKP KSVVAPPGAP KKEHVNVVFI GHVDAGKSTI GGQIMYLTGM VD KRTLEKY EREAKEKNRE TWYLSWALDT NQEERDKGKT VEVGRAYFET EKKHFTILDA PGHKSFVPNM IGGASQADLA VLV ISARKG EFETGFEKGG QTREHAMLAK TAGVKHLIVL INKMDDPTVN WSNERYEECK EKLVPFLKKV GFNPKKDIHF MPCS GLTGA NLKEQSDFCP WYIGLPFIPY LDNLPNFNRS VDGPIRLPIV DKYKDMGTVV LGKLESGSIC KGQQLVMMPN KHNVE VLGI LSDDVETDTV APGENLKIRL KGIEEEEILP GFILCDPNNL CHSGRTFDAQ IVIIEHKSII CPGYNAVLHI HTCIEE VEI TALICLVDKK SGEKSKTRPR FVKQDQVCIA RLRTAGTICL ETFKDFPQMG RFTLRDEGKT IAIGKVLKLV PEKD

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Macromolecule #46: P-site tRNA

MacromoleculeName: P-site tRNA / type: rna / ID: 46 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.436551 KDa
SequenceString:
GUCUCCGUAG UGUAGCGGUU AUCACGUUCG CCUAACACGC GAAAGGUCCU CGGUUCGAAA CCGGGCGGAA ACACCA

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Macromolecule #47: E-site tRNA

MacromoleculeName: E-site tRNA / type: rna / ID: 47 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.102275 KDa
SequenceString:
GCCCGGAUAG CUCAGCUGUA GAGCAUCAGA CUUUUAAUCU GAGGGUCCAG GGUUCAAGUC CCUGUUCGGG CGCCA

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Macromolecule #48: 28S ribosomal RNA

MacromoleculeName: 28S ribosomal RNA / type: rna / ID: 48 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 1.148115375 MDa
SequenceString: CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ...String:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGGGGGGC CCAAGUCCUU CUGAUCGAGG CCCAGCCCGU GGACGGUGUG AGGCCGGUAG CG GCCCCCG GCGCGCCGGG CCCGGGUCUU CCCGGAGUCG GGUUGCUUGG GAAUGCAGCC CAAAGCGGGU GGUAAACUCC AUC UAAGGC UAAAUACCGG CACGAGACCG AUAGUCAACA AGUACCGUAA GGGAAAGUUG AAAAGAACUU UGAAGAGAGA GUUC AAGAG GGCGUGAAAC CGUUAAGAGG UAAACGGGUG GGGUCCGCGC AGUCCGCCCG GAGGAUUCAA CCCGGCGGCG CGGUC CGGC CGUGCCGGCG GGGCGGAUCU CCCGCUCCCG UCGGCGGGGG ACCGCCCCCC GGCCGGCGAC CGGCCGCCGC CGGGCG CAU UUCCACCGCG GCGGUGCGCC GCGACCGGCU CCGGGACGGC UGGGAAGGCC CCGGCGGGGA AGGUGGCCCG GGGGCCC CG GGUGUUACAG CCCCGCCCCG GCAGCAGCAG CUCGCCGAAU CCCGGGGCCG AGGGAGCGAG ACCCGUGCGC CGCGCUCU C CCCCCUCGGG GGGGCCGGGC CACCCCUCCC ACGGCGCGAC CGCUGGGGCG GACUGUCCAG UGCGCCCCGG GCGGGUCGC GCCGUCGGGC CCGGGGGGCC ACGCGUCCCC CGCGAGCGCA CGGGGUCGGC GGCGAUGUCG GCUACCCACC CGACCCGUCU UGAAACACG GACCAAGGAG UCUAACACGU GCGCGAGUCG GGGGCUACGA AAGCCGCCGU GGCGCAAUGA AGGUGAAGGC C GGCGGCGC GCCGCCGGCC GAGGUGGGAU CCCGAGGCCU UCCAGUCCGC CGAGGGCGCA CCACCGGCCC GUCUCGCCCG CC GCGCCGG GGAGGUGGAG CACGAGCGCA CGUGUUAGGA CCCGAAAGAU GGUGAACUAU GCCUGGGCAG GGCGAAGCCA GAG GAAACU CUGGUGGAGG UCCGUAGCGG UCCUGACGUG CGAAUCGGUC GUCCGACCUG GGUAUAGGGG CGAAAGACUA AUCG AACCA UCUAGUAGCU GGUUCCCUCC AAAGUUUCCC UCAGGAUAGC UGGCGCUCUC CGCAGUUUUA UCCGGUAAAG CGAAU GAUU AGAGGUCUUG GGGCCGAAAC GAUCUCAACC UAUUCUCAAA CUUUAAAUGG GUAAGAAGCC CGGCUCGCUG GUGUGG AGC CGGGCGUGGA AUGCGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAACUG GCGCUGCGGG AUGAACCGAA CGCCGGG UU AAGGCGCCCG AUGCCGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUUGA UAUAGACAGC AGGAUGGUGG CCAUGGAA G UCGGAAUCCG CUAAGGAGUG UGUAACAACU CACCUGCCGA AUCAACUAGC CCUGAAAAUG GAUGGCGCUG GAGCGUCGG GCCCAUACCC GGCCGUCGCU GGCAGUCGGC AGAGCGAGAA AGAGAGUGGC GCGGACGCUA CGCCGCGACG AGUAGGAGGG CCGCUGCGG UGAGCCUUGA AGCCUAGGGC GCGGGCCCGG GUGGAGCCGC CGCAGGUGCA GAUCUUGGUG GUAGUAGCAA A UAUUCAAA CGAGAACUUU GAAGGCCGAA GUGGAGAAGG GUUCCAUGUG AACAGCAGUU GAACAUGGGU CAGUCGGUCC UG AGAGAUG GGCGAGCGCC GUUCCGAAGG GACGGGCGAU GGCCUCCGUU GCCCUCAGCC GAUCGAAAGG GAGUCGGGUU CAG AUCCCC GAAUCCGGAG UGGCGGAGAU GGGCGCCGCG AGGCGUCCAG UGCGGUAACG CGACCGAUCC CGGAGAAGCC GGCG GGAGC CCCGGGGAGA GUUCUCUUUU CUUUGUGAAG GGCAGGGCGC CCUGGAAUGG GUUCGCCCCG AGAGAGGGGC CCGUG CCUU GGAAAGCGUC GCGGUUCCGG CGGCGUCCGG UGAGCUCUCG CUGGCCCUUG AAAAUCCGGG GGAGAGGGUG UAAAUC UCG CGCCGGGCCG UACCCAUAUC CGCAGCAGGU CUCCAAGGUG AACAGCCUCU GGCAUGUUGG AACAAUGUAG GUAAGGG AA GUCGGCAAGC CGGAUCCGUA ACUUCGGGAU AAGGAUUGGC UCUAAGGGCU GGCAGCCGAC UUAGAACUGG UGCGGACC A GGGGAAUCCG ACUGUUUAAU UAAAACAAAG CAUCGCGAAG GCCCGCGGCG GGUGUUGACG CGAUGUGAUU UCUGCCCAG UGCUCUGAAU GUCAAAGUGA AGAAAUUCAA UGAAGCGCGG GUAAACGGCG GGAGUAACUA UGACUCUCUU AAGGUAGCCA AAUGCCUCG UCAUCUAAUU AGUGACGCGC AUGAAUGGAU GAACGAGAUU CCCACUGUCC CUACCUACUA UCCAGCGAAA C CACAGCCA AGGGAACGGG CUUGGCGGAA UCAGCGGGGA AAGAAGACCC UGUUGAGCUU GACUCUAGUC UGGCACGGUG AA GAGACGU UUUUUCACUG ACCCGGUGAG GCGGGGGGGC GAGCCGGGCU CUCGCUUCUG GCGCCAAGCG CCCGGCCGGC CGG GCGCGA CCCGCUCCGG GGACAGUGCC AGGUGGGGAG UUUGACUGGG GCGGUACACC UGUCAAACGG UAACGCAGGU GUCC UAAGG CGAGCUCAGG GAGGACAGAA ACCUCCCGUG GAGCAGAAGG GCAAAAGCUC GCUUGAUCUU GAUUUUCAGU ACGAA UACA GACCGUGAAA GCGGGGCCUC ACGAUCCUUC UGACCUUUUG GGUUUUAAGC AGGAGGUGUC AGAAAAGUUA CCACAG GGA UAACUGGCUU GUGGCGGCCA AGCGUUCAUA GCGACGUCGC UUUUUGAUCC UUCGAUGUCG GCUCUUCCUA UCAUUGU GA AGCAGAAUUC ACCAAGCGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGUCGUG AGACAGGU U AGUUUUACCC UACUGAUGAU GUGUUGUUGC CAUGGUAAUC CUGCUCAGUA CGAGAGGAAC CGCAGGUUCA GACAUUUGG UGUAUGUGCU UGGCUGAGGA GCCAAUGGGG CGAAGCUACC AUCUGUGGGA UUAUGACUGA ACGCCUCUAA GUCAGAAUCC CGCCCAGGC GGAACGAUAG CGCCGGAGCC UCGGUUGGCC UCGGAUAGCC GGUCCCCCGC CGGGGUCCGG UGCGGAGAGC C CUUCGUCC CGGGAAACGG GGCGGCCGGA AAGGGGGCCG CCCUCUCGCC CGUCACGCAC CGCACGUUCG UGGGGAACCU GG CGCUAAA CCAUUCGUAG ACGACCUGCU UCUGGGUCGG GGUUUCGUAC GUAGCAGAGC AGCUGCUGCG AUCUAUUGAA AGU CAGCCC UCGACACAAG GGUUUGU

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Macromolecule #49: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 49 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 38.691914 KDa
SequenceString:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

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Macromolecule #50: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 50 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 50.143648 KDa
SequenceString:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCU

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Macromolecule #51: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 51 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 602.778875 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC UAAUACAUGC CGACGGGCGC UGACCCCCUU CGCGGGGGGG AUGCGUGCAU UUAUCAGAUC AAAACCAACC CG GUCAGCC CCUCUCCGGC CCCGGCCGGG GGGCGGGCGC CGGCGGCUUU GGUGACUCUA GAUAACCUCG GGCCGAUCGC ACG CCCCCC GUGGCGGCGA CGACCCAUUC GAACGUCUGC CCUAUCAACU UUCGAUGGUA GUCGCCGUGC CUACCAUGGU GACC ACGGG UGACGGGGAA UCAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGGCUA CCACAUCCAA GGAAGGCAGC AGGCG CGCA AAUUACCCAC UCCCGACCCG GGGAGGUAGU GACGAAAAAU AACAAUACAG GACUCUUUCG AGGCCCUGUA AUUGGA AUG AGUCCACUUU AAAUCCUUUA ACGAGGAUCC AUUGGAGGGC AAGUCUGGUG CCAGCAGCCG CGGUAAUUCC AGCUCCA AU AGCGUAUAUU AAAGUUGCUG CAGUUAAAAA GCUCGUAGUU GGAUCUUGGG AGCGGGCGGG CGGUCCGCCG CGAGGCGA G CCACCGCCCG UCCCCGCCCC UUGCCUCUCG GCGCCCCCUC GAUGCUCUUA GCUGAGUGUC CCGCGGGGCC CGAAGCGUU UACUUUGAAA AAAUUAGAGU GUUCAAAGCA GGCCCGAGCC GCCUGGAUAC CGCAGCUAGG AAUAAUGGAA UAGGACCGCG GUUCUAUUU UGUUGGUUUU CGGAACUGAG GCCAUGAUUA AGAGGGACGG CCGGGGGCAU UCGUAUUGCG CCGCUAGAGG U GAAAUUCU UGGACCGGCG CAAGACGGAC CAGAGCGAAA GCAUUUGCCA AGAAUGUUUU CAUUAAUCAA GAACGAAAGU CG GAGGUUC GAAGACGAUC AGAUACCGUC GUAGUUCCGA CCAUAAACGA UGCCGACUGG CGAUGCGGCG GCGUUAUUCC CAU GACCCG CCGGGCAGCU UCCGGGAAAC CAAAGUCUUU GGGUUCCGGG GGGAGUAUGG UUGCAAAGCU GAAACUUAAA GGAA UUGAC GGAAGGGCAC CACCAGGAGU GGAGCCUGCG GCUUAAUUUG ACUCAACACG GGAAACCUCA CCCGGCCCGG ACACG GACA GGAUUGACAG AUUGAUAGCU CUUUCUCGAU UCCGUGGGUG GUGGUGCAUG GCCGUUCUUA GUUGGUGGAG CGAUUU GUC UGGUUAAUUC CGAUAACGAA CGAGACUCUG GCAUGCUAAC UAGUUACGCG ACCCCCGAGC GGUCGGCGUC CCCCAAC UU CUUAGAGGGA CAAGUGGCGU UCAGCCACCC GAGAUUGAGC AAUAACAGGU CUGUGAUGCC CUUAGAUGUC CGGGGCUG C ACGCGCGCUA CACUGACUGG CUCAGCGUGU GCCUACCCUA CGCCGGCAGG CGCGGGUAAC CCGUUGAACC CCAUUCGUG AUGGGGAUCG GGGAUUGCAA UUAUUCCCCA UGAACGAGGA AUUCCCAGUA AGUGCGGGUC AUAAGCUUGC GUUGAUUAAG UCCCUGCCC UUUGUACACA CCGCCCGUCG CUACUACCGA UUGGAUGGUU UAGUGAGGCC CUCGGAUCGG CCCCGCCGGG G UCGGCCCA CGGCCCUGGC GGAGCGCUGA GAAGACGGUC GAACUUGACU AUCUAGAGGA AGUAAAAGUC GUAACAAGGU UU CCGUAGG UGAACCUGCG GAAGGAUCAU UA

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Macromolecule #85: mRNA (UGA stop codon)

MacromoleculeName: mRNA (UGA stop codon) / type: rna / ID: 85 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 4.753857 KDa
SequenceString:
UCAAAGUUUG AGCUC

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Macromolecule #88: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 88 / Number of copies: 282 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #89: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 89 / Number of copies: 8 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #90: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 90 / Number of copies: 1 / Formula: GCP
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.2 mg/mL
BufferpH: 7.4
Component:
ConcentrationNameFormula
50.0 mMHEPES
100.0 mMPotassium acetateKOAc
5.0 mMMagnessium acetateMg(OAc)2
1.0 mMDTT
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 5.0 nm / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK III
Details: 3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution..

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Number real images: 2611 / Average exposure time: 1.0 sec. / Average electron dose: 30.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Calibrated magnification: 104478 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal magnification: 59000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 211664
CTF correctionSoftware - Name: Gctf (ver. 0.5)
Startup modelType of model: EMDB MAP
EMDB ID:

3039


Details: Low-pass filtered to 30 A
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.65 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 61752
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
Final 3D classificationSoftware - Name: RELION (ver. 1.4)
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: RECIPROCAL / Protocol: OTHER / Overall B value: 63.3 / Target criteria: FSCaverage
Output model

PDB-5lzt:
Structure of the mammalian ribosomal termination complex with eRF1 and eRF3.

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