+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-36024 | |||||||||
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Title | Methylcrotonoyl-CoA carboxylase core-Dimer | |||||||||
Map data | ||||||||||
Sample |
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Keywords | MCC / Mitochondria / CYTOSOLIC PROTEIN | |||||||||
Function / homology | Function and homology information : / methylcrotonoyl-CoA carboxylase / methylcrotonoyl-CoA carboxylase complex / methylcrotonoyl-CoA carboxylase activity / Defective HLCS causes multiple carboxylase deficiency / Biotin transport and metabolism / L-leucine catabolic process / branched-chain amino acid catabolic process / Branched-chain amino acid catabolism / coenzyme A metabolic process ...: / methylcrotonoyl-CoA carboxylase / methylcrotonoyl-CoA carboxylase complex / methylcrotonoyl-CoA carboxylase activity / Defective HLCS causes multiple carboxylase deficiency / Biotin transport and metabolism / L-leucine catabolic process / branched-chain amino acid catabolic process / Branched-chain amino acid catabolism / coenzyme A metabolic process / mitochondrial matrix / mitochondrion / ATP binding / cytosol Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.16 Å | |||||||||
Authors | Liu DS / Su JY | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Methylcrotonoyl-CoA carboxylase core-Dimer Authors: Liu DS / Su JY | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_36024.map.gz | 62.5 MB | EMDB map data format | |
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Header (meta data) | emd-36024-v30.xml emd-36024.xml | 12.5 KB 12.5 KB | Display Display | EMDB header |
Images | emd_36024.png | 109.1 KB | ||
Filedesc metadata | emd-36024.cif.gz | 5 KB | ||
Others | emd_36024_half_map_1.map.gz emd_36024_half_map_2.map.gz | 116 MB 115.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-36024 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-36024 | HTTPS FTP |
-Validation report
Summary document | emd_36024_validation.pdf.gz | 1.2 MB | Display | EMDB validaton report |
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Full document | emd_36024_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | emd_36024_validation.xml.gz | 14.2 KB | Display | |
Data in CIF | emd_36024_validation.cif.gz | 16.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36024 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36024 | HTTPS FTP |
-Related structure data
Related structure data | 8j73MC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_36024.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.0979 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_36024_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_36024_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Methylcrotonoyl-CoA carboxylase core-Dimer
Entire | Name: Methylcrotonoyl-CoA carboxylase core-Dimer |
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Components |
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-Supramolecule #1: Methylcrotonoyl-CoA carboxylase core-Dimer
Supramolecule | Name: Methylcrotonoyl-CoA carboxylase core-Dimer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Homo sapiens (human) |
-Macromolecule #1: Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
Macromolecule | Name: Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO / EC number: methylcrotonoyl-CoA carboxylase |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 61.406027 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MWAVLRLALR PCARASPAGP RAYHGDSVAS LGTQPDLGSA LYQENYKQMK ALVNQLHERV EHIKLGGGEK ARALHISRGK LLPRERIDN LIDPGSPFLE LSQFAGYQLY DNEEVPGGGI ITGIGRVSGV ECMIIANDAT VKGGAYYPVT VKKQLRAQEI A MQNRLPCI ...String: MWAVLRLALR PCARASPAGP RAYHGDSVAS LGTQPDLGSA LYQENYKQMK ALVNQLHERV EHIKLGGGEK ARALHISRGK LLPRERIDN LIDPGSPFLE LSQFAGYQLY DNEEVPGGGI ITGIGRVSGV ECMIIANDAT VKGGAYYPVT VKKQLRAQEI A MQNRLPCI YLVDSGGAYL PRQADVFPDR DHFGRTFYNQ AIMSSKNIAQ IAVVMGSCTA GGAYVPAMAD ENIIVRKQGT IF LAGPPLV KAATGEEVSA EDLGGADLHC RKSGVSDHWA LDDHHALHLT RKVVRNLNYQ KKLDVTIEPS EEPLFPADEL YGI VGANLK RSFDVREVIA RIVDGSRFTE FKAFYGDTLV TGFARIFGYP VGIVGNNGVL FSESAKKGTH FVQLCCQRNI PLLF LQNIT GFMVGREYEA EGIAKDGAKM VAAVACAQVP KITLIIGGSY GAGNYGMCGR AYSPRFLYIW PNARISVMGG EQAAN VLAT ITKDQRAREG KQFSSADEAA LKEPIIKKFE EEGNPYYSSA RVWDDGIIDP ADTRLVLGLS FSAALNAPIE KTDFGI FRM UniProtKB: Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.3 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: INSILICO MODEL |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.16 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 1474 |
Initial angle assignment | Type: RANDOM ASSIGNMENT |
Final angle assignment | Type: ANGULAR RECONSTITUTION |