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Yorodumi- EMDB-30461: cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-30461 | ||||||||||||
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Title | cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 | ||||||||||||
Map data | cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 | ||||||||||||
Sample |
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Biological species | Severe acute respiratory syndrome coronavirus 2 / Homo sapiens (human) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.3 Å | ||||||||||||
Authors | Guo L / Bi WW / Zhang YY / Yan RH / Li YN / Zhou Q / Dang BB | ||||||||||||
Funding support | China, 3 items
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Citation | Journal: Cell Res / Year: 2021 Title: Engineered trimeric ACE2 binds viral spike protein and locks it in "Three-up" conformation to potently inhibit SARS-CoV-2 infection. Authors: Liang Guo / Wenwen Bi / Xinling Wang / Wei Xu / Renhong Yan / Yuanyuan Zhang / Kai Zhao / Yaning Li / Mingfeng Zhang / Xia Cai / Shibo Jiang / Youhua Xie / Qiang Zhou / Lu Lu / Bobo Dang / | ||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_30461.map.gz | 83.9 MB | EMDB map data format | |
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Header (meta data) | emd-30461-v30.xml emd-30461.xml | 10.7 KB 10.7 KB | Display Display | EMDB header |
Images | emd_30461.png | 34.3 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-30461 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-30461 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_30461.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.087 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2
Entire | Name: cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 |
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Components |
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-Supramolecule #1: cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2
Supramolecule | Name: cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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-Supramolecule #2: SARS-CoV-2
Supramolecule | Name: SARS-CoV-2 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: Severe acute respiratory syndrome coronavirus 2 |
Recombinant expression | Organism: Homo sapiens (human) / Recombinant cell: HEK293 |
-Supramolecule #3: T-ACE2
Supramolecule | Name: T-ACE2 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2 |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Homo sapiens (human) / Recombinant cell: HEK293 |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
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Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0.6) / Number images used: 59822 |