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- EMDB-23121: Cryo-EM structure of the human 39S mitoribosomal subunit in compl... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-23121 | |||||||||
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Title | Cryo-EM structure of the human 39S mitoribosomal subunit in complex with RRFmt and EF-G2mt. | |||||||||
![]() | Cryo-EM structure of the human 39S mitoribosomal subunit in complex with RRFmt and EF-G2mt. | |||||||||
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Function / homology | ![]() mitochondrial transcription / mitochondrial translational elongation / mitochondrial translational termination / microprocessor complex / translation release factor activity, codon nonspecific / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / mitochondrial large ribosomal subunit / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters ...mitochondrial transcription / mitochondrial translational elongation / mitochondrial translational termination / microprocessor complex / translation release factor activity, codon nonspecific / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / mitochondrial large ribosomal subunit / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / ribosome disassembly / peptidyl-tRNA hydrolase / mitochondrial small ribosomal subunit / aminoacyl-tRNA hydrolase activity / mitochondrial ribosome / mitochondrial translation / ribosomal large subunit binding / anatomical structure morphogenesis / RNA processing / Mitochondrial protein degradation / rescue of stalled ribosome / cellular response to leukemia inhibitory factor / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / fibrillar center / large ribosomal subunit / double-stranded RNA binding / cell junction / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / endonuclease activity / cytosolic large ribosomal subunit / mitochondrial inner membrane / nuclear body / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / mitochondrial matrix / ribonucleoprotein complex / translation / protein domain specific binding / nucleotide binding / GTPase activity / mRNA binding / synapse / nucleolus / GTP binding / apoptotic process / positive regulation of DNA-templated transcription / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.15 Å | |||||||||
![]() | Agrawal E / Koripella R | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Distinct mechanisms of the human mitoribosome recycling and antibiotic resistance. Authors: Ravi Kiran Koripella / Ayush Deep / Ekansh K Agrawal / Pooja Keshavan / Nilesh K Banavali / Rajendra K Agrawal / ![]() Abstract: Ribosomes are recycled for a new round of translation initiation by dissociation of ribosomal subunits, messenger RNA and transfer RNA from their translational post-termination complex. Here we ...Ribosomes are recycled for a new round of translation initiation by dissociation of ribosomal subunits, messenger RNA and transfer RNA from their translational post-termination complex. Here we present cryo-EM structures of the human 55S mitochondrial ribosome (mitoribosome) and the mitoribosomal large 39S subunit in complex with mitoribosome recycling factor (RRF) and a recycling-specific homolog of elongation factor G (EF-G2). These structures clarify an unusual role of a mitochondria-specific segment of RRF, identify the structural distinctions that confer functional specificity to EF-G2, and show that the deacylated tRNA remains with the dissociated 39S subunit, suggesting a distinct sequence of events in mitoribosome recycling. Furthermore, biochemical and structural analyses reveal that the molecular mechanism of antibiotic fusidic acid resistance for EF-G2 is markedly different from that of mitochondrial elongation factor EF-G1, suggesting that the two human EF-Gs have evolved diversely to negate the effect of a bacterial antibiotic. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 227 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 65.5 KB 65.5 KB | Display Display | ![]() |
Images | ![]() | 171.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7l20MC ![]() 7l08C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | |
EM raw data | ![]() Data size: 2.3 TB Data #1: Aligned single-frame particles of human mitochondrial 55S-EF-Gmt complex [picked particles - single frame - processed]) |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Cryo-EM structure of the human 39S mitoribosomal subunit in complex with RRFmt and EF-G2mt. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.07325 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
+Entire : Cryo-EM structure of Mammalian Mitochondrial ribosome complex wit...
+Supramolecule #1: Cryo-EM structure of Mammalian Mitochondrial ribosome complex wit...
+Macromolecule #1: 16S rRNA mitochondrial
+Macromolecule #2: tRNAval
+Macromolecule #3: 39S ribosomal protein L2, mitochondrial
+Macromolecule #4: 39S ribosomal protein L4, mitochondrial
+Macromolecule #5: 39S ribosomal protein L9, mitochondrial
+Macromolecule #6: 39S ribosomal protein L13, mitochondrial
+Macromolecule #7: 39S ribosomal protein L14, mitochondrial
+Macromolecule #8: 39S ribosomal protein L15, mitochondrial
+Macromolecule #9: 39S ribosomal protein L17, mitochondrial
+Macromolecule #10: 39S ribosomal protein L20, mitochondrial
+Macromolecule #11: 39S ribosomal protein L21, mitochondrial
+Macromolecule #12: 39S ribosomal protein L22, mitochondrial
+Macromolecule #13: 39S ribosomal protein L27, mitochondrial
+Macromolecule #14: 39S ribosomal protein L28, mitochondrial
+Macromolecule #15: 39S ribosomal protein L47, mitochondrial
+Macromolecule #16: 39S ribosomal protein L30, mitochondrial
+Macromolecule #17: 39S ribosomal protein L32, mitochondrial
+Macromolecule #18: 39S ribosomal protein L33, mitochondrial
+Macromolecule #19: 39S ribosomal protein L34, mitochondrial
+Macromolecule #20: 39S ribosomal protein L35, mitochondrial
+Macromolecule #21: 39S ribosomal protein L36, mitochondrial
+Macromolecule #22: 39S ribosomal protein L40, mitochondrial
+Macromolecule #23: 39S ribosomal protein L43, mitochondrial
+Macromolecule #24: 39S ribosomal protein L46, mitochondrial
+Macromolecule #25: 39S ribosomal protein L49, mitochondrial
+Macromolecule #26: 39S ribosomal protein L51, mitochondrial
+Macromolecule #27: cDNA FLJ76418, highly similar to Homo sapiens mitochondrial ribos...
+Macromolecule #28: 39S ribosomal protein L55, mitochondrial
+Macromolecule #29: Ribosomal protein 63, mitochondrial
+Macromolecule #30: Growth arrest and DNA damage-inducible proteins-interacting protein 1
+Macromolecule #31: 39S ribosomal protein S18a, mitochondrial
+Macromolecule #32: 39S ribosomal protein L11, mitochondrial
+Macromolecule #33: 39S ribosomal protein L10, mitochondrial
+Macromolecule #34: 39S ribosomal protein L16, mitochondrial
+Macromolecule #35: Mitochondrial ribosomal protein L18, isoform CRA_b
+Macromolecule #36: 39S ribosomal protein L23, mitochondrial
+Macromolecule #37: 39S ribosomal protein L24, mitochondrial
+Macromolecule #38: 39S ribosomal protein L3, mitochondrial
+Macromolecule #39: 39S ribosomal protein L37, mitochondrial
+Macromolecule #40: 39S ribosomal protein L38, mitochondrial
+Macromolecule #41: 39S ribosomal protein L39, mitochondrial
+Macromolecule #42: 39S ribosomal protein L41, mitochondrial
+Macromolecule #43: 39S ribosomal protein L42, mitochondrial
+Macromolecule #44: 39S ribosomal protein L44, mitochondrial
+Macromolecule #45: 39S ribosomal protein L45, mitochondrial
+Macromolecule #46: 39S ribosomal protein L48, mitochondrial
+Macromolecule #47: 39S ribosomal protein L50, mitochondrial
+Macromolecule #48: 39S ribosomal protein L53, mitochondrial
+Macromolecule #49: 39S ribosomal protein L54, mitochondrial
+Macromolecule #50: Peptidyl-tRNA hydrolase ICT1, mitochondrial
+Macromolecule #51: 39S ribosomal protein S30, mitochondrial
+Macromolecule #52: 39S ribosomal protein L19, mitochondrial
+Macromolecule #53: 39 S P-site finger
+Macromolecule #54: 39S ribosomal protein L12, mitochondrial
+Macromolecule #55: Ribosome-recycling factor, mitochondrial
+Macromolecule #56: Ribosome-releasing factor 2, mitochondrial
+Macromolecule #57: MAGNESIUM ION
+Macromolecule #58: ZINC ION
+Macromolecule #59: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: DIRECT ELECTRON DE-16 (4k x 4k) / Average electron dose: 71.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: OTHER |
Electron optics | Illumination mode: OTHER / Imaging mode: OTHER |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.15 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 132008 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
-Atomic model buiding 1
Refinement | Protocol: OTHER |
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Output model | ![]() PDB-7l20: |