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- EMDB-21030: Cryo-EM structure of the Acinetobacter baumannii Ribosome: 70S wi... -

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Basic information

Entry
Database: EMDB / ID: EMD-21030
TitleCryo-EM structure of the Acinetobacter baumannii Ribosome: 70S with P-site tRNA
Map dataComplete map of Acinetobacter baumannii 70S ribosome with P-site bound tRNA created by combining three separate focused refinements (50S, 30S core and 30S head)
Sample
  • Complex: Acinetobacter baumannii 70S ribosome with P-site tRNA
    • Protein or peptide: x 48 types
    • RNA: x 5 types
  • Ligand: x 5 types
Function / homology
Function and homology information


large ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / transferase activity / tRNA binding / ribosome / rRNA binding / structural constituent of ribosome / translation ...large ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / transferase activity / tRNA binding / ribosome / rRNA binding / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L21, conserved site ...Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein S6, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L14P, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein S16 domain superfamily / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19
Similarity search - Domain/homology
Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein bS21 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL35 ...Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein bS21 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL20
Similarity search - Component
Biological speciesAcinetobacter baumannii AB0057 (bacteria) / Acinetobacter sp. CIP 51.11 (bacteria) / Acinetobacter beijerinckii ANC 3835 (bacteria) / Acinetobacter baumannii (strain AB0057) (bacteria) / Acinetobacter sp. ANC 4470 (bacteria) / Acinetobacter sp. NIPH 899 (bacteria) / Acinetobacter baumannii (bacteria) / Acinetobacter sp. 809848 (bacteria) / Acinetobacter rudis CIP 110305 (bacteria) / Acinetobacter sp. CIP 102082 (bacteria) / Acinetobacter sp. ANC 5600 (bacteria) / Acinetobacter sp. 263903-1 (bacteria) / Acinetobacter venetianus (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.04 Å
AuthorsMorgan CE / Yu EW
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) United States
CitationJournal: mBio / Year: 2020
Title: Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development.
Authors: Christopher E Morgan / Wei Huang / Susan D Rudin / Derek J Taylor / James E Kirby / Robert A Bonomo / Edward W Yu /
Abstract: Antimicrobial resistance is a major health threat as it limits treatment options for infection. At the forefront of this serious issue is , a Gram-negative opportunistic pathogen that exhibits the ...Antimicrobial resistance is a major health threat as it limits treatment options for infection. At the forefront of this serious issue is , a Gram-negative opportunistic pathogen that exhibits the remarkable ability to resist antibiotics through multiple mechanisms. As bacterial ribosomes represent a target for multiple distinct classes of existing antimicrobial agents, we here use single-particle cryo-electron microscopy (cryo-EM) to elucidate five different structural states of the ribosome, including the 70S, 50S, and 30S forms. We also determined interparticle motions of the 70S ribosome in different tRNA bound states using three-dimensional (3D) variability analysis. Together, our structural data further our understanding of the ribosome from and other Gram-negative pathogens and will enable structure-based drug discovery to combat antibiotic-resistant bacterial infections. is a severe nosocomial threat largely due to its intrinsic antibiotic resistance and remarkable ability to acquire new resistance determinants. The bacterial ribosome serves as a major target for modern antibiotics and the design of new therapeutics. Here, we present cryo-EM structures of the 70S ribosome, revealing several unique species-specific structural features that may facilitate future drug development to combat this recalcitrant bacterial pathogen.
History
DepositionNov 25, 2019-
Header (metadata) releaseJan 8, 2020-
Map releaseFeb 5, 2020-
UpdateDec 2, 2020-
Current statusDec 2, 2020Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.45
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.45
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6v39
  • Surface level: 0.45
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_21030.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComplete map of Acinetobacter baumannii 70S ribosome with P-site bound tRNA created by combining three separate focused refinements (50S, 30S core and 30S head)
Voxel sizeX=Y=Z: 1.064 Å
Density
Contour LevelBy AUTHOR: 0.45 / Movie #1: 0.45
Minimum - Maximum-1.6323706 - 4.511605
Average (Standard dev.)0.006775898 (±0.091348335)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 544.768 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0641.0641.064
M x/y/z512512512
origin x/y/z0.0000.0000.000
length x/y/z544.768544.768544.768
α/β/γ90.00090.00090.000
start NX/NY/NZ29921935
NX/NY/NZ271234450
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS512512512
D min/max/mean-1.6324.5120.007

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Supplemental data

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Additional map: Complete map of Acinetobacter baumannii 70S ribosome with...

Fileemd_21030_additional_1.map
AnnotationComplete map of Acinetobacter baumannii 70S ribosome with P-site bound tRNA created by combining three separate focused refinements (50S, 30S core and 30S head); local resolution
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Additional map: Focused refinement of 30S head

Fileemd_21030_additional_2.map
AnnotationFocused refinement of 30S head
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Additional map: Focused refinement of 50S ribosomal subunit

Fileemd_21030_additional_3.map
AnnotationFocused refinement of 50S ribosomal subunit
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Additional map: Focused refinement of 30S core

Fileemd_21030_additional_4.map
AnnotationFocused refinement of 30S core
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire : Acinetobacter baumannii 70S ribosome with P-site tRNA

EntireName: Acinetobacter baumannii 70S ribosome with P-site tRNA
Components
  • Complex: Acinetobacter baumannii 70S ribosome with P-site tRNA
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • RNA: 23s ribosomal RNA
    • RNA: 5s ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L32
    • RNA: 16s Ribosomal RNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 30S ribosomal protein S21
    • RNA: tRNA-met
    • RNA: mRNA 5'-AUG-3'
  • Ligand: ZINC ION
  • Ligand: N-FORMYLMETHIONINE
  • Ligand: MAGNESIUM ION
  • Ligand: SODIUM IONSodium
  • Ligand: water

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Supramolecule #1: Acinetobacter baumannii 70S ribosome with P-site tRNA

SupramoleculeName: Acinetobacter baumannii 70S ribosome with P-site tRNA / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
Source (natural)Organism: Acinetobacter baumannii AB0057 (bacteria)
Molecular weightTheoretical: 2.3 MDa

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Macromolecule #1: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter sp. CIP 51.11 (bacteria)
Molecular weightTheoretical: 6.10531 KDa
SequenceString:
MRDKIRLVSS AGTGYFYTTT KNKRTMPEKM EIKKFDPKIR QHVIFKEAKI K

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Macromolecule #2: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter beijerinckii ANC 3835 (bacteria)
Molecular weightTheoretical: 5.192247 KDa
SequenceString:
MKRTFQPSEL KRKRVHGFRA RMATKAGRQV LARRRAKGRH SLTV

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Macromolecule #3: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 7.424098 KDa
SequenceString:
MAKLKTRRGA AKRFKATANG FKRKQAFKRH ILTKKSAKRI RQLRGCVMVH VSDVASVRRM CPYI

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Macromolecule #4: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter sp. ANC 4470 (bacteria)
Molecular weightTheoretical: 4.276201 KDa
SequenceString:
MKVQASVKKI CGSCKVIRRN GVIRVICSAE PRHKQRQG

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Macromolecule #7: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 30.346115 KDa
SequenceString: MPIQKCKPTS PGRRFVEKVV HDHLHKGAPY APLVEAKKRT GGRNNNGHIT TRHVGGGHKQ HYRIVDFKRN KDGVPAVVER IEYDPNRTA HIALLKYADG ERRYIIAPKG LRAGDKVQSG NDAPIRPGNC LPLRNMPIGS TLHNVELKIG KGAQLARSAG A SVQLLGRD ...String:
MPIQKCKPTS PGRRFVEKVV HDHLHKGAPY APLVEAKKRT GGRNNNGHIT TRHVGGGHKQ HYRIVDFKRN KDGVPAVVER IEYDPNRTA HIALLKYADG ERRYIIAPKG LRAGDKVQSG NDAPIRPGNC LPLRNMPIGS TLHNVELKIG KGAQLARSAG A SVQLLGRD GSYAIIRLRS GEMRKVHVEC RAVIGEVSNQ ENNLRSLGKA GAARWRGVRP TVRGMAMNPI DHPHGGGEGR NK GIQPVSP WGQKAKGYKT RTNKRTTKMI IRDRRVK

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Macromolecule #8: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 22.512639 KDa
SequenceString: MAIGLVGRKC GMTRIFTDAG VSVPVTVIEV DPNRITQIKT LETDGYQAVQ VTTGERRESR VTNAQKGHFA KAGVAAGRLV KEFRVTEAE LEGREVGGTI GVDLFTVGQI VDVTGQSKGK GFQGGVKRWN FRTQDATHGN SVSHRVLGST GQNQTPGRVF K GKKMAGHL ...String:
MAIGLVGRKC GMTRIFTDAG VSVPVTVIEV DPNRITQIKT LETDGYQAVQ VTTGERRESR VTNAQKGHFA KAGVAAGRLV KEFRVTEAE LEGREVGGTI GVDLFTVGQI VDVTGQSKGK GFQGGVKRWN FRTQDATHGN SVSHRVLGST GQNQTPGRVF K GKKMAGHL GDERVTVQGL EIVSVDTERS VLVVKGAIPG ATGGDVIVRP TIKA

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Macromolecule #9: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 21.583904 KDa
SequenceString: MNLKTVSGSA VELSEVAFGR EFNEALVHQV VTAYLAGGRQ GTRAHKSRAD VSGGGKKPFR QKGTGRARAG SIRSPIWVGG GKTFAARPQ DWSQKVNRKM YRGAMQCILA ELVRQDRLVL VEEFAVAAPK TKELLAKLND LNAARALIVT DAVDENLYLA A RNLPHVDV ...String:
MNLKTVSGSA VELSEVAFGR EFNEALVHQV VTAYLAGGRQ GTRAHKSRAD VSGGGKKPFR QKGTGRARAG SIRSPIWVGG GKTFAARPQ DWSQKVNRKM YRGAMQCILA ELVRQDRLVL VEEFAVAAPK TKELLAKLND LNAARALIVT DAVDENLYLA A RNLPHVDV VDATAIDPVS LIAFDKVVMS VAAAKKIEVE LG

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Macromolecule #10: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 20.051471 KDa
SequenceString:
MARLKARYND ELKAKLQEEL SIKNVMEIPR ITKITLNMGV GAAATDKKLL DGAVADMQLI AGQKPVVTLA RKSIAGFKIR DGWPIGCKV TLRGDQMYEF LDRLISIAIP RIRDFRGFSA KSFDGRGNYS MGLKEQIVFP EIDFDKIDRI RGMDITITTT A RTDDEGRA LMRAFGFPFK

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Macromolecule #11: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 19.126047 KDa
SequenceString:
MSRVAKAPVT VPNGVTVTQN GRQVEVKGSK GTLSFNLHAL VELKQEEGKL QLAPAKESKD AWMQAGTARA VLNNLVKGVS EGFERKLQL VGVGYKAAVK GTVVNLNLGY SHPIDYALPE GVTAETPTAT EIILKSANKQ LLGQVAAEIR AYRSPEPYKG K GVRYSDEV ILRKEAKKK

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Macromolecule #12: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 15.800955 KDa
SequenceString:
MDVILLQRIK NLGKLGDKVS VKAGYGRNFL IPQGKAVAAT EANTAAFEAR RAELEKQEAE VLAAAQARAE QLNEVNIVIT AKAGDEGKL FGSIGTRDIA DALTNAGLTV DRAEVRLPNG ALRHTGEFNI AIQLHHDVVA EVLVTIVSE

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Macromolecule #13: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 15.981466 KDa
SequenceString:
MKTLSAKPAE VQHDWFVVDA TGKTLGRLAT EIARRLRGKH KTSYTPHVDT GDYIIVINAE QVQVTGNKAL DKKYYRHTEF PGGLKETNF EKLVAHKPEE IFERAVKGML PKGPLGYAMI KKMKVYAGSE HPHAAQQPQV LDI

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Macromolecule #14: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter sp. NIPH 899 (bacteria)
Molecular weightTheoretical: 13.523863 KDa
SequenceString:
MIQTETMLDV ADNSGARRVQ CIKVLGGSHR RYASVGDIIK VTVKEAIPRA RVKKGDVMNA VVVRTKFGIR RPDGSVIRFD DNAAVILNN NKAPIATRIF GPVTRELRTE QFMKIISLAP EVL

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Macromolecule #15: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 15.509915 KDa
SequenceString:
MTLRLNELAP AEGAKREHRR LGRGIGSGVG KTGGRGIKGQ KSRKSGGVRP GFEGGQTAIY RRLPKFGFTS QIALKTAEVR LSELSKVEG DIVSLETLKA ANVVRRDQIR ARIVLSGEIT RAFTVQGVAL TKGAKAAIEA AGGKVEE

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Macromolecule #16: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 15.498312 KDa
SequenceString:
MLQPKRTKFR KVHKGRNTGL AHRGSTVSFG SIAIKATERG RMTARQIEAA RRTISRRIKR GGKIFIRVFP DKPITEKPLE VRMGNGKGN VEYWVCEIKP GKILYEIEGV NEDLAREAFA LAAAKLPFKT TIVTRTVM

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Macromolecule #17: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 14.022215 KDa
SequenceString:
MRHRNSGVKL GRTSSHRKAM FENLANSLFE HELIKTTLPK AKELRRVAEP LITLAKNDTV ANRRLAFART RNAATVGKLF TVLGPRYKE RNGGYLRVLK AGFRAGDAAP MAYVELVDRE VNTSAE

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Macromolecule #18: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 12.443341 KDa
SequenceString:
MNEKKQSRLR RAKSTRLHIR ALGATRLCVN RTPRHIYAQV ISADGGKVLA QASTLDASLR SGTTGNIEAA TKVGALIAER AKAAGVTKV AFDRSGFKYH GRIKALADAA REGGLEF

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Macromolecule #19: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 13.619865 KDa
SequenceString:
MSGKHPLVQA IENSQLKTDL PEFAPGDTVV VQVKVKEGDR ERLQAFEGVV IAKKNRGLNS AFTVRKISSG VGVERVFQTH SPVVAKIEV KRRGDVRRAK LYYLRDLSGK AARIREKLPA RKA

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Macromolecule #20: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (bacteria)
Molecular weightTheoretical: 13.468946 KDa
SequenceString:
MARVKRGVVA HRRHKKILAR AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQKKRQFRAL WIARINAGAR QNGLSYSRMI DGLKKAQVI IDRRVLADIA MHDAVAFAAL AEKAKGALAA

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Macromolecule #21: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 11.496364 KDa
SequenceString:
MYAVIQSGGK QHRVVEGETL KVELLKAESG ATITFDDVLM VVNGDNIQIG APVVAGAKVT AEVIGHGRHD KIRIIKMRRR KHYRKQQGH RQWFTELKIT GISG

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Macromolecule #22: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter sp. 809848 (bacteria)
Molecular weightTheoretical: 11.828931 KDa
SequenceString:
MEVTAKLRGA AISAQKARLV ADLIRGKSVA HALNILNFSN KKAAVLVKKA LESAIANAEH NNSLDVDDLK VSTIYVDEGM SLKRIMPRA KGRADRITKR TCHITVKVGV

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Macromolecule #23: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 11.607392 KDa
SequenceString:
MNNERIYQVL KGPVFSEKAQ VLGDTAGVQV FKVDINATKL EIKKAVEKLF GVEVVKVNTT ITKGKTKRFG RTLGRRSDVK KAYVTLKAG QDVEMADLGD TAESAAE

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Macromolecule #24: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 11.187929 KDa
SequenceString:
MAKIKKGDQV IVIAGKEKGK QGTVLSVSED RVKVEGLNLV KKHQKPNRVT GAEGGIVTQE ASLHISNVAI LNATTQKADR VGYQVIDGV KTRVYKSTGE SVAVAK

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Macromolecule #25: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 10.942593 KDa
SequenceString:
MANFVLNAQA RAEDKQGKGA SRRLRRESLV PAIIYGGNAE PVAVTLELRE LVKALESNVF FEEVVEIKVG DKVENVKIQA LQRHPAKNT PMHADFKRA

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Macromolecule #26: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 9.072387 KDa
SequenceString:
MATKKAGGST KNGRDSNPKM LGVKVYGGQT VTAGNIIVRQ RGTEFHAGAN VGMGRDHTLF ATADGVVKFE VKGQFGRRYV KVETV

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Macromolecule #27: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter rudis CIP 110305 (bacteria)
Molecular weightTheoretical: 9.125756 KDa
SequenceString:
MSKVCQVTGK RPVVGNNVSH ANNKTKRRFE PNLHHHRFWL ESEKRFVRLR LTTKGMRIID KLGIEKVVAD LRAQGQKI

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Macromolecule #28: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 7.449678 KDa
SequenceString:
MKTKDLREKS VEELKALLDE QQLNQFRLRM AKATGQLGKS HEVQVARKTI ARIKTLLTEK QGNGQ

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Macromolecule #29: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter sp. CIP 102082 (bacteria)
Molecular weightTheoretical: 6.654788 KDa
SequenceString:
MKTIKVTQTK SSSHRLKNHK LCLQGLGLRR IGHTVEVQDT PSNRGMINKV YYMVSVEE

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Macromolecule #30: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 7.096888 KDa
SequenceString:
MAVQQNRKSR SRRDMRRSHD ALTENALTVD QATGETHRRH HVTKDGFYRG RQLFAKAADA E

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Macromolecule #32: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (bacteria)
Molecular weightTheoretical: 27.680357 KDa
SequenceString: MADYNVSMRD LLQAGAHFGH QTRFWNPKMR QYIFGARNKI HIINLEHTVP ALNDALNFAN QLASKKNKVL FVGTKRAASN IIREQAQRA GQPYVDHRWL GGMLTNWKTL RQSINRLKDL QTQSQDGTFA KLTKREALER TREMEKLERS LGGVKNMGGL P DALFVIDV ...String:
MADYNVSMRD LLQAGAHFGH QTRFWNPKMR QYIFGARNKI HIINLEHTVP ALNDALNFAN QLASKKNKVL FVGTKRAASN IIREQAQRA GQPYVDHRWL GGMLTNWKTL RQSINRLKDL QTQSQDGTFA KLTKREALER TREMEKLERS LGGVKNMGGL P DALFVIDV DHEAIAIKEA KNLGIPVIGI VDTNSNPDNV DYVIPGNDDA IRAVTLYASA MADAILAGKE YAQSQANAQA KG DDAAKDA SEA

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Macromolecule #33: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (bacteria)
Molecular weightTheoretical: 27.972461 KDa
SequenceString: MGQKVHPIGI RLGVVKRHNA NWYANPKQYA EYLLKDLQVR EFLTKKLKNA MVSNILIERP SGAAKVTIST ARPGIVIGKK GEDIEKLQR ELTNIMGVPA QVSINEIDRP DLDARLVAEA IASQLEKRVM FRRAMKRAVQ NTMRAGAKGI KVEVSGRLGG A EIARTEWY ...String:
MGQKVHPIGI RLGVVKRHNA NWYANPKQYA EYLLKDLQVR EFLTKKLKNA MVSNILIERP SGAAKVTIST ARPGIVIGKK GEDIEKLQR ELTNIMGVPA QVSINEIDRP DLDARLVAEA IASQLEKRVM FRRAMKRAVQ NTMRAGAKGI KVEVSGRLGG A EIARTEWY REGRVPLHTL RADIDYATMR AETTYGTIGV KVWIFRGEIL GGMKQVMNPA PAEERPAKRG RGRGEGQERR GR RGDRAAD KGE

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Macromolecule #34: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 23.311818 KDa
SequenceString: MARYIGPKCK LSRREGTDLQ LKSGVKPFDV KTKKANKAPG QHGQARGGKQ SEYSLQLREK QKVRRIYGVL ERQFSNYYKE AARVKGATG ENLLKLLESR LDNVVYRMGF GSTRAEARQL VSHRSITLNG RRVNIASIQV KAGDVIAVHE GAKQQLRIKN A IELAAQRG ...String:
MARYIGPKCK LSRREGTDLQ LKSGVKPFDV KTKKANKAPG QHGQARGGKQ SEYSLQLREK QKVRRIYGVL ERQFSNYYKE AARVKGATG ENLLKLLESR LDNVVYRMGF GSTRAEARQL VSHRSITLNG RRVNIASIQV KAGDVIAVHE GAKQQLRIKN A IELAAQRG IPAWIEVDHS KLEGTFKAAP DRSDLPAEIN ESLIVELYSK

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Macromolecule #35: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 17.181766 KDa
SequenceString:
MAKVEQNEGL VEKLVAVDRV AKVVKGGRIF SFTALTVVGD GNGRVGFGRG KAREVPAAIS KALEAARRNM ITVDLAGTTL QHPVNARHG ASRVYMQPAS EGTGVIAGGA MRAVLEAAGV HNVLAKCYGS TNAANVVNAT FKGLRDMTSP EKVAAKRGKS V EEIQG

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Macromolecule #36: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 14.986952 KDa
SequenceString:
MRHYEIVLLV HPDQSDQVVG MVERYISQIK EADGQIHRLE DWGRRQLAYP INKIHKAHYI LMNVECGQST LDELEELFRY NDAIIRNLI IRREHAITEE SLLAKSAEEK RARKAQREEA QQVAQEAE

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Macromolecule #37: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 17.733699 KDa
SequenceString:
MPRRRVVAAR EILPDPKFSS QTIAKFMNHV MQDGKKSIAE SIVYGALERV QEKNKVDPVE FFETTLEKVR PMVEVKARRV GGATYQVPM EVRPSRRTAL AMRWLVDAAA KRSEKTMALR LAGELLDAAE GKGAAIKKRE DVHRMAEANK AFSHYRF

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Macromolecule #38: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 14.250667 KDa
SequenceString:
MSMQDTVADM LTRVRNAQMA KKQTVSMPSS KLKVAIANVL QQEGYISNVE VAQEETKSTL TITLKYFEGK PVIEMVKRVS RPGLRQYRG KDKLPSVKQG LGIAIVSTSK GIMTDRAARA AGIGGEVIAF VS

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Macromolecule #39: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (bacteria)
Molecular weightTheoretical: 14.28761 KDa
SequenceString:
MATNYGTGRR KTATARVFLS AGTGKLVINN RTLEQYFGRE TARMVVRQPL ELLEATEKYD LYITVKGGGI GGQAGAIRHG ITRALIAAD ETLKPVLRQA GFVTRDAREV ERKKLGLRKA RKRPQFSKR

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Macromolecule #40: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter sp. ANC 5600 (bacteria)
Molecular weightTheoretical: 11.718531 KDa
SequenceString:
MSNQRIRIRL KSFDHRLIDQ SAQEIVETAK RTGAQVCGPI PMPTRIERFN VLTSPHVNKD ARDQYEIRTY KRLIDIVQPT DKTVDALMK LDLAAGVDVQ IALG

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Macromolecule #41: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter sp. 263903-1 (bacteria)
Molecular weightTheoretical: 13.558512 KDa
SequenceString:
MAKDTRTRKK VTRTVSEGVA HIHASFNNTI VTITDRQGNA LAWATSGGQG FRGSRKSTPF AAQVAAEVAG KAALDYGLKN LDVLVKGPG PGRESAVRAL GAVGYKINSI TDVTPIPHNG CRPPKKRRV

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Macromolecule #42: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 13.797134 KDa
SequenceString:
MATTNQLIRK GRTTLVEKSK VPALKACPQR RGVCTRVYTT TPKKPNSAMR KVCRVRLTSG FEVSSYIGGE GHNLQEHSVV LIRGGRVKD LPGVRYHTVR GSLDCAGVKD RNQSRSKYGA KRPKK

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Macromolecule #43: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 13.295635 KDa
SequenceString:
MARIAGVNIP DNKHAVISLT YIFGIGRHTA KNILAAVGIT ETTKIRELDD AQLDAIRAEV AKVPTEGDLR REISMNIKRL MDLGCYRGL RHRRSLPVRG QRTKTNARTR KGPRKPIKK

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Macromolecule #44: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 11.438427 KDa
SequenceString:
MAKKGMINRE LKREKTVAKY AAKRAELKAT IANVNASDEE RFEAMLKLQA LPRNASPVRL RNRCGLTGRP HGYFRKFGLS RNKLRDTVM QGDVPGVVKA SW

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Macromolecule #45: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 10.1456 KDa
SequenceString:
MALTNADRAE IIAKFARAEN DTGSPEVQVA LLTAQINDLQ GHFKAHKHDH HSRRGLIRMV NQRRKLLDYL NGKDHERYTA LIGALGLRR

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Macromolecule #46: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (bacteria)
Molecular weightTheoretical: 11.22306 KDa
SequenceString:
MLPMVGAGII RKNYKAFIMV VIRLARGGAK KRPFYQIVVT DSRNARDGRF IERIGFFNPT AQGQAEKLRL DADRFAHWVS QGAQPSERV ASLAAQAKKA TA

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Macromolecule #47: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 9.543101 KDa
SequenceString:
MSEKTVRTLT GKVVSDKMDK SIVVLIERRV QHPLYGKSIR RSTKLHAHDE NNVAKIGDVV TIKESRPISK TKAWTLVEVV EAAAE

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Macromolecule #48: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter venetianus (bacteria)
Molecular weightTheoretical: 9.009452 KDa
SequenceString:
MARFYRRRKF CRFTAENVAY IDYKDIDTLK QYITENGKIV PSRITGTKAR YQRQLALAIK QARYLSLIPY TDNHK

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Macromolecule #49: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 10.206957 KDa
SequenceString:
MPRSLKKGPF VDAHLFAKVE AAVASNSRKP IKTWSRRSMI LPDFVGLTIS VHNGRNHVPV IVTEHMVGHK LGEFAPTRTY RGHGVDKKS KR

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Macromolecule #50: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter baumannii (strain AB0057) (bacteria)
Strain: AB0057
Molecular weightTheoretical: 9.72342 KDa
SequenceString:
MANSAQAKKR ARQNVKARKH NASLRSMVRT YIKRTLSAIA GGDYAVATEA YKKAVPVIDR MADKGIIHKN KAARHKSRLN AQVKALAN

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Macromolecule #51: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acinetobacter sp. 263903-1 (bacteria)
Molecular weightTheoretical: 8.474033 KDa
SequenceString:
MPQVKLKEGE PVDVAIRRFK RSCEKAGVLA DVRKREFYEK PTQERKRKKA AAVKRYQKKL ARESVRTTRL Y

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Macromolecule #5: 23s ribosomal RNA

MacromoleculeName: 23s ribosomal RNA / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Acinetobacter baumannii AB0057 (bacteria)
Molecular weightTheoretical: 945.29925 KDa
SequenceString: GUUGUAUAGU CAAGUAAUUA AGUGCAUGUG GUGGAUGCCU UGGCAGUCAG AGGCGAUGAA AGACGUGAUA GCCUGCGAAA AGCUCCGGG GAGGCGGCAA AUAUCCUUUG AUCCGGAGAU GUCUGAAUGG GGGAACCCAC CUACUUUAAG GUAGGUAUUG C AACAUGAA ...String:
GUUGUAUAGU CAAGUAAUUA AGUGCAUGUG GUGGAUGCCU UGGCAGUCAG AGGCGAUGAA AGACGUGAUA GCCUGCGAAA AGCUCCGGG GAGGCGGCAA AUAUCCUUUG AUCCGGAGAU GUCUGAAUGG GGGAACCCAC CUACUUUAAG GUAGGUAUUG C AACAUGAA UACAUAGUGU UGCAAGGCGA ACGAGGGGAA GUGAAACAUC UCAGUACCCU UAGGAAAAGA AAUCAAUUGA GA UUCCCUC AGUAGCGGCG AGCGAACGGG GAUCAGCCCA UUAAGUUAUG UGUGUUUUAG UGGAACGCUC UGGGAAGUGC GAA CGUAGA GGGUGAUAUU CCCGUACACG AAAGGGCACA CAUAAUGAUG ACGAGUAGGG CGAGGCACGU GAAACCUUGU CUGA AUAUG GGGGGACCAU CCUCCAAGGC UAAAUACUCC UGACUGACCG AUAGUGAACC AGUACCGUGA GGGAAAGGCG AAAAG AACC CCUGUGAGGG GAGUGAAAUA GAUCCUGAAA CCGCAUGCAU ACAAGCAGUG GGAGCACCUU CGUGGUGUGA CUGCGU ACC UUUUGUAUAA UGGGUCAGCG ACUUAUAUUC AGUAGCGAGG UUAACCGUAU AGGGGAGCCG UAGAGAAAUC GAGUCUU AA UAGGGCGUUU AGUUGCUGGG UAUAGACCCG AAACCAGGCG AUCUAUCCAU GAGCAGGUUG AAGGUUGGGU AACACUAA C UGGAGGACCG AACCCACUGU CGUUGAAAAG CCAGGGGAUG ACUUGUGGAU AGGGGUGAAA GGCUAAUCAA GCCUGGUGA UAGCUGGUUC UCCCCGAAAG CUAUUUAGGU AGCGCCUCGG ACGAAUACCA UAGGGGGUAG AGCACUGUUU CGGCUAGGGG GUCAUCCCG ACUUACCAAA CCGAUGCAAA CUCCGAAUAC CUAUGAGUAC UAUCCGGGAG ACAGACUGCG GG(PSU)GCUA AC GUCCGUAGUC AAGAGGAAAA CAAUCCAGAC CGCCAGCUAA GGCCCCAAAA UCAUAGUUAA GUGGGAAACG AUGUGGGA A GGCAUAGACA GCUAGGAGGU UGGCUUAGAA GCAGCCACCC UUUAAAGAAA GCGUAAUAGC UCACUAGUCG AGUCGGCCU GCGCGGAAGA UGUAACGGGG CUAAAACUAU GUGCCGAAGC UGCGGAUGUA UACUUUGUAU ACGUGGUAGG GGAGCGUUCU GUAAGCCGA UGAAGGUGUG UUGAGAAGCA UGCUGGAGGU AUCAGAAGUG CGAAUGCUGA CGUGAGUAAC GACAAAACGG G UGAAAAAC CCGUUCGCCG AAAGACCAAG GGUUCCAGUC CAACGUUAAU CGGGGCUGGG UGAGUCGACC CCUAAGGCGA GG CCGAAAG GCGUAGUCGA UGGGAAAAUG GUUAAUAUUC CAUUACUUCU GUGUAAUGCG AUGAGAGGAC GGAGAAGGCU AAA UCAGCC UGGCGUUGGU UGUCCAGGUG AAAGGAUGUA GGCAUGUAUC UUAGGCAAAU CCGGGGUACU CUAUGCUGAG AUCU GAUAG CAAGCUGUAC UUGUACAGCG AAGUGGUUGA UGCCAUGCUU CCAGGAAAAG UCUCUAAGCU UCAGUUACAC AGGAA UCGU ACCCGAAACC GACACAGGUG GUCAGGUCGA GUAGACCAAG GCGCUUGAGA GAACUCUGCU GAAGGAACUA GGCAAA AUG GUACCGUAAC UUCGGGAGAA GGUACGCUGU UGUUGGUGAU GGAACUCGCU UCCUGAGCUG ACGACAGCCG CAGAAAC CA GGCCGCUGCA ACUGUUUAUU AAAAACAUAG CACUCUGCAA ACACGAAAGU GGACGUAUAG GGUGUGAUGC CUGCCCGG U GCUGGAAGGU UAAUUGAUGG GGUUAGCGUA AGCGAAGCUC UUGAUCGAAG CCCCAGUAAA CGGCGGCCG(PSU) AAC (3TD)A(PSU)AAC GGUCCUAAGG UAGCGAAA(5MU)U CCUUGUCGGG UAAGUUCCGA CCUGCACGAA UGGCAUAAUG AU GGCGGCG CUGUCUCCAG CAGAGGCUCA GUGAAAUCGA AAUCGCUGUG (6MZ)AGAUGCAGU GUACCCGCGG CUAGACGGA AAGACCCCGU (7MG)AACCUUUAC UGCAGCUUGA CACUGAACUU UGACCUUACU UGUGUAGGAU AGGUGGGAGG CUUUGA AGC UGGAACGCUA GUUCCAGUGG AGCCGUCCUU GAAAUACCAC CCUGGUAAUG UUGAGGUUCU AACUCUGUCC CGUGAUC CG GGACGAGGAC CGUGUCUGGU GGGUAGUUUG ACUG(OMG)GGCGG UCUCCUCCUA AAGAGUAACG GAGGAGUACG AAGG UGCGC UCAGCGUGGU CGGAAAUCAC GCGUAGAGUA UAAAGGCAAA AGCGCGCUUA ACUGCGAGAC CCACAAGUCG AGCAG GUAC GAAAGUAGGU CUUAGUGAUC CGGUGGUUCU GUAUGGAAGG GCCAUCGCUC AACGGAUAAA AGGUACUCUG (2MG)G GAUAACA GGC(PSU)GAUACC GCCCAAGAGU UCAUAUCGAC GGCGGUGUUU GGCACCUCG(2MA) (PSU)GUCGGCUCA U CUCAUCCU GGGGCUGAAG CAGGUCCCAA GGGUAUGGC(OMU) GUUCGCCAUU UAAAGAGGUA CGCGAGC(PSU)GG GUUU AGAAC GUCGUGAGAC AGU(PSU)CGGUCC CUAUCUACCG UGGGCGCUGG AAAUUUGAGA GGAUCUGCUC CUAGUACGAG A GGACCAGA GUGGACGAAC CUCUGGUGUA CCGGUUGUGA CGCCAGUCGC AUCGCCGGGU AGCUAUGUUC GGAAGGGAUA AC CGCUGAA AGCAUCUAAG CGGGAAGCCU ACCUCAAGAU AAGAUUUCCC UAGGACUUUA UGUCCUCUAA AGAGCCGUUC GAG ACUAGG ACGUUGAUAG GUUGGAUGUG GAAGCAUAGU GAUAUGUGAA GCUGACCAAU ACUAAUUGCU CGUGAGGCUU GACU AUACA ACACCCAAGC AGUUG

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Macromolecule #6: 5s ribosomal RNA

MacromoleculeName: 5s ribosomal RNA / type: rna / ID: 6 / Number of copies: 1
Source (natural)Organism: Acinetobacter baumannii (bacteria)
Molecular weightTheoretical: 36.996992 KDa
SequenceString:
GCUGGCGACC AUAGCAAGAG UGAACCACCU GAUCCCUUCC CGAACUCAGA AGUGAAACCU CUUAGCGCUG AUGGUAGUGU GGGAUUACC CAUGUGAGAG UAAGUCAUCG CCAGCU

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Macromolecule #31: 16s Ribosomal RNA

MacromoleculeName: 16s Ribosomal RNA / type: rna / ID: 31 / Number of copies: 1
Source (natural)Organism: Acinetobacter baumannii AB0057 (bacteria)
Molecular weightTheoretical: 500.297531 KDa
SequenceString: UUUAACUGAA GAGUUUGAUC AUGGCUCAGA UUGAACGCUG GCGGCAGGCU UAACACAUGC AAGUCGAGCG GGGGAAGGUA GCUUGCUAC CGGACCUAGC GGCGGACGGG UGAGUAAUGC UUAGGAAUCU GCCUAUUAGU GGGGGACAAC AUCUCGAAAG G GAUGCUAA ...String:
UUUAACUGAA GAGUUUGAUC AUGGCUCAGA UUGAACGCUG GCGGCAGGCU UAACACAUGC AAGUCGAGCG GGGGAAGGUA GCUUGCUAC CGGACCUAGC GGCGGACGGG UGAGUAAUGC UUAGGAAUCU GCCUAUUAGU GGGGGACAAC AUCUCGAAAG G GAUGCUAA UACCGCAUAC GUCCUACGGG AGAAAGCAGG GGAUCUUCGG ACCUUGCGCU AAUAGAUGAG CCUAAGUCGG AU UAGCUAG UUGGUGGGGU AAAGGCCUAC CAAGGCGACG AUCUGUAGCG GGUCUGAGAG GAUGAUCCGC CACACUGGGA CUG AGACAC GGCCCAGACU CCUACGGGAG GCAGCAGUGG GGAAUAUUGG ACAAUGGGGG GAACCCUGAU CCAGCCAUGC CGCG UGUGU GAAGAAGGCC UUAUGGUUGU AAAGCACUUU AAGCGAGGAG GAGGCUACUC UAGUUAAUAC CUAGGGAUAG UGGAC GUUA CUCGCAGAAU AAGCACCGGC UAACUCUG(PSU)G CCAGCAGCC(7MG) CGGUAAUACA GAGGGUGCGA GCGUUAAU C GGAUUUACUG GGCGUAAAGC GUGCGUAGGC GGCUUAUUAA GUCGGAUGUG AAAUCCCCGA GCUUAACUUG GGAAUUGCA UUCGAUACUG GUGAGCUAGA GUAUGGGAGA GGAUGGUAGA AUUCCAGGUG UAGCGGUGAA AUGCGUAGAG AUCUGGAGGA AUACCGAUG GCGAAGGCAG CCAUCUGGCC UAAUACUGAC GCUGAGGUAC GAAAGCAUGG GGAGCAAACA GGAUUAGAUA C CCUGGUAG UCCAUGCCGU AAACGAUGUC UACUAGCCGU UGGGGCCUUU GAGGCUUUAG UGGCGCAGCU AACGCGAUAA GU AGACCGC CUGGGGAGUA CGGUCGCAAG ACUAAAACUC AAAUGAAUUG ACGGGGGCCC GCACAAGCGG UGGAGCAUGU GGU UUAAUU CGAU(2MG)(5MC)AACG CGAAGAACCU UACCUGGCCU UGACAUACUA GAAACUUUCC AGAGAUGGAU UGGUGC CUU CGGGAAUCUA GAUACAGGUG CUGCAUGGCU GUCGUCAGCU CGUGUCGUGA GAUGUUGGGU UAAGUCCCGC AACGAGC GC AACCCUUUUC CUUACUUGCC AGCAUUUCGG AUGGGAACUU UAAGGAUACU GCCAGUGACA AACUGGAGGA AGGCGGGG A CGACGUCAAG UCAUCAUG(2MG)C CCUUACGGCC AGGGCUACAC ACGUGCUACA AUGGUCGGUA CAAAGGGUUG CUACA CAGC GAUGUGAUGC UAAUCUCAAA AAGCCGAUCG UAGUCCGGAU UGGAGUCUGC AACUCGACUC CAUGAAGUCG GAAUCG CUA GUAAUCGCGG AUCAGAAUGC CGCGGUGAAU ACGUUCCCGG GCCUUGUACA CACCG(4OC)CCGU CACACCAUGG GAG UUUGUU GCACCAGAAG UAGCUAGCCU AACUGCAAAG AGGGCGGUUA CCACGGUGUG GCCGAUGACU GGGGUGAAGU CG (UR3)AACAAG GUAGCCGUAG GGG(MA6)(MA6)CCUGC GGCUGGAUCA CCUCCUUAAC GAA

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Macromolecule #52: tRNA-met

MacromoleculeName: tRNA-met / type: rna / ID: 52 / Number of copies: 1
Source (natural)Organism: Acinetobacter baumannii AB0057 (bacteria)
Molecular weightTheoretical: 24.760799 KDa
SequenceString:
GGCUAUG(4SU)AG CUCAGUUGG(H2U) UAGAGCACCG CACUCAUAAU GCGGGGGUCA CAAG(5MU)(PSU)CAAG UCUC GUCAU AGCCACCA

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Macromolecule #53: mRNA 5'-AUG-3'

MacromoleculeName: mRNA 5'-AUG-3' / type: rna / ID: 53 / Number of copies: 1
Source (natural)Organism: Acinetobacter baumannii AB0057 (bacteria)
Molecular weightTheoretical: 935.62 Da
SequenceString:
AUG

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Macromolecule #54: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 54 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #55: N-FORMYLMETHIONINE

MacromoleculeName: N-FORMYLMETHIONINE / type: ligand / ID: 55 / Number of copies: 1 / Formula: FME
Molecular weightTheoretical: 177.221 Da
Chemical component information

ChemComp-FME:
N-FORMYLMETHIONINE / N-Formylmethionine

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Macromolecule #56: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 114 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #57: SODIUM ION

MacromoleculeName: SODIUM ION / type: ligand / ID: 57 / Number of copies: 1
Molecular weightTheoretical: 22.99 Da

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Macromolecule #58: water

MacromoleculeName: water / type: ligand / ID: 58 / Number of copies: 273 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final 3D classificationSoftware - Name: RELION / Software - details: Focused classification
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.04 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 24306

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Atomic model buiding 1

Initial modelPDB ID:
Output model

PDB-6v39:
Cryo-EM structure of the Acinetobacter baumannii Ribosome: 70S with P-site tRNA

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