+
Open data
-
Basic information
Entry | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Composite structure of Dynein-Dynactin-JIP3-LIS1 | ||||||||||||
![]() | Composite map of Dynein-Dynactin-JIP3-LIS1 filtered to 10 angstrom resolution | ||||||||||||
![]() |
| ||||||||||||
![]() | Dynein / AAA-Atpase / p150 / LIS1 / MOTOR PROTEIN / Dynactin / JIP3 | ||||||||||||
Function / homology | ![]() intracellular transport of viral protein in host cell / corpus callosum morphogenesis / secretory vesicle / establishment of planar polarity of embryonic epithelium / microtubule cytoskeleton organization involved in establishment of planar polarity / nitric-oxide synthase inhibitor activity / ameboidal-type cell migration / interneuron migration / 1-alkyl-2-acetylglycerophosphocholine esterase complex / retrograde axonal transport of mitochondrion ...intracellular transport of viral protein in host cell / corpus callosum morphogenesis / secretory vesicle / establishment of planar polarity of embryonic epithelium / microtubule cytoskeleton organization involved in establishment of planar polarity / nitric-oxide synthase inhibitor activity / ameboidal-type cell migration / interneuron migration / 1-alkyl-2-acetylglycerophosphocholine esterase complex / retrograde axonal transport of mitochondrion / negative regulation of DNA strand resection involved in replication fork processing / Gap junction degradation / Formation of annular gap junctions / Regulation of actin dynamics for phagocytic cup formation / EPHB-mediated forward signaling / Adherens junctions interactions / VEGFA-VEGFR2 Pathway / Cell-extracellular matrix interactions / RHO GTPases Activate WASPs and WAVEs / MAP2K and MAPK activation / UCH proteinases / Clathrin-mediated endocytosis / RHOF GTPase cycle / maintenance of centrosome location / microtubule sliding / dynactin complex / Regulation of PLK1 Activity at G2/M Transition / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / transport along microtubule / visual behavior / platelet activating factor metabolic process / intraciliary retrograde transport / WASH complex / radial glia-guided pyramidal neuron migration / F-actin capping protein complex / cerebral cortex neuron differentiation / microtubule organizing center organization / positive regulation of intracellular transport / dynein light chain binding / central region of growth cone / acrosome assembly / positive regulation of embryonic development / negative regulation of filopodium assembly / reelin-mediated signaling pathway / regulation of metaphase plate congression / establishment of centrosome localization / anterograde axonal protein transport / positive regulation of cytokine-mediated signaling pathway / motile cilium assembly / cortical microtubule organization / dynein heavy chain binding / establishment of spindle localization / Activation of BIM and translocation to mitochondria / astral microtubule / axonemal dynein complex / positive regulation of spindle assembly / nuclear membrane disassembly / ciliary tip / cytoskeleton-dependent cytokinesis / layer formation in cerebral cortex / cell tip / auditory receptor cell development / structural constituent of postsynaptic actin cytoskeleton / vesicle transport along microtubule / positive regulation of dendritic spine morphogenesis / microtubule plus-end / dense body / Intraflagellar transport / stem cell division / negative regulation of nitric oxide biosynthetic process / MAP-kinase scaffold activity / stereocilium / myeloid leukocyte migration / dynein complex / regulation of G protein-coupled receptor signaling pathway / negative regulation of phosphorylation / Neutrophil degranulation / P-body assembly / COPI-independent Golgi-to-ER retrograde traffic / negative regulation of JNK cascade / microtubule-dependent intracellular transport of viral material towards nucleus / minus-end-directed microtubule motor activity / JUN kinase binding / barbed-end actin filament capping / dynein light intermediate chain binding / microtubule plus-end binding / cytoplasmic dynein complex / brain morphogenesis / regulation of cell morphogenesis / motile cilium / regulation of lamellipodium assembly / retrograde axonal transport / enzyme inhibitor activity / nuclear migration / RHO GTPases activate IQGAPs / RHO GTPases Activate Formins Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 10.0 Å | ||||||||||||
![]() | Singh K / Lau CK / Manigrasso G / Gassmann R / Carter AP | ||||||||||||
Funding support | ![]()
| ||||||||||||
![]() | ![]() Title: Molecular mechanism of dynein-dynactin complex assembly by LIS1. Authors: Kashish Singh / Clinton K Lau / Giulia Manigrasso / José B Gama / Reto Gassmann / Andrew P Carter / ![]() ![]() Abstract: Cytoplasmic dynein is a microtubule motor vital for cellular organization and division. It functions as a ~4-megadalton complex containing its cofactor dynactin and a cargo-specific coiled-coil ...Cytoplasmic dynein is a microtubule motor vital for cellular organization and division. It functions as a ~4-megadalton complex containing its cofactor dynactin and a cargo-specific coiled-coil adaptor. However, how dynein and dynactin recognize diverse adaptors, how they interact with each other during complex formation, and the role of critical regulators such as lissencephaly-1 (LIS1) protein (LIS1) remain unclear. In this study, we determined the cryo-electron microscopy structure of dynein-dynactin on microtubules with LIS1 and the lysosomal adaptor JIP3. This structure reveals the molecular basis of interactions occurring during dynein activation. We show how JIP3 activates dynein despite its atypical architecture. Unexpectedly, LIS1 binds dynactin's p150 subunit, tethering it along the length of dynein. Our data suggest that LIS1 and p150 constrain dynein-dynactin to ensure efficient complex formation. | ||||||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 917.7 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 45.2 KB 45.2 KB | Display Display | ![]() |
Images | ![]() | 45.7 KB | ||
Filedesc metadata | ![]() | 14.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 457.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 456.8 KB | Display | |
Data in XML | ![]() | 8.6 KB | Display | |
Data in CIF | ![]() | 10 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8ptkMC ![]() 8pqvC ![]() 8pqwC ![]() 8pqyC ![]() 8pqzC ![]() 8pr0C ![]() 8pr1C ![]() 8pr2C ![]() 8pr3C ![]() 8pr4C ![]() 8pr5C C: citing same article ( M: atomic model generated by this map |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Composite map of Dynein-Dynactin-JIP3-LIS1 filtered to 10 angstrom resolution | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.059 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-
Sample components
+Entire : Composite structure of Dynein-Dynactin-JIP3-LIS1
+Supramolecule #1: Composite structure of Dynein-Dynactin-JIP3-LIS1
+Supramolecule #2: Dynactin subunits
+Supramolecule #3: Dynein and JIP3
+Macromolecule #1: Platelet-activating factor acetylhydrolase IB subunit beta
+Macromolecule #2: ARP1 actin related protein 1 homolog A
+Macromolecule #3: Actin, cytoplasmic 1
+Macromolecule #4: Arp11
+Macromolecule #5: Capping protein (Actin filament) muscle Z-line, alpha 1
+Macromolecule #6: F-actin-capping protein subunit beta
+Macromolecule #7: Dynactin subunit 2
+Macromolecule #8: Dynactin subunit 3
+Macromolecule #9: Dynactin subunit 1
+Macromolecule #10: Dynactin 6
+Macromolecule #11: Dynactin subunit 5
+Macromolecule #12: C-Jun-amino-terminal kinase-interacting protein 3
+Macromolecule #13: Dynactin subunit 4
+Macromolecule #14: Dynein light chain 1, cytoplasmic
+Macromolecule #15: Cytoplasmic dynein 1 heavy chain 1
+Macromolecule #16: Cytoplasmic dynein 1 intermediate chain 2
+Macromolecule #17: Cytoplasmic dynein 1 light intermediate chain 2
+Macromolecule #18: Dynein light chain Tctex-type 1
+Macromolecule #19: Dynein light chain roadblock-type 1
+Macromolecule #20: ADENOSINE-5'-DIPHOSPHATE
+Macromolecule #21: ADENOSINE-5'-TRIPHOSPHATE
+Macromolecule #22: ZINC ION
+Macromolecule #23: MAGNESIUM ION
+Macromolecule #24: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.2 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 53.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 0.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Startup model | Type of model: INSILICO MODEL |
---|---|
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 10.0 Å / Resolution method: OTHER / Number images used: 700290 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |