4UN7
| THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA BEFORE INCUBATION IN 5MM MN (STATE 1) | Descriptor: | 25MER, HOMING ENDONUCLEASE I-DMOI, ZINC ION | Authors: | Molina, R, Stella, S, Redondo, P, Gomez, H, Marcaida, M.J, Orozco, M, Prieto, J, Montoya, G. | Deposit date: | 2014-05-26 | Release date: | 2014-12-03 | Last modified: | 2024-01-10 | Method: | X-RAY DIFFRACTION (2.7 Å) | Cite: | Visualizing Phosphodiester-Bond Hydrolysis by an Endonuclease. Nat.Struct.Mol.Biol., 22, 2015
|
|
8AA5
| Cryo-EM structure of the strand transfer complex of the TnsB transposase (type V-K CRISPR-associated transposon) | Descriptor: | LE_PolyA, LE_Target, RE_PolyA, ... | Authors: | Tenjo-Castano, F, Sofos, N, Lopez-Mendez, B, Stutzke, L.S, Fuglsang, A, Stella, S, Montoya, G. | Deposit date: | 2022-06-30 | Release date: | 2022-10-19 | Method: | ELECTRON MICROSCOPY (2.46 Å) | Cite: | Structure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon. Nat Commun, 13, 2022
|
|
8AXB
| Cryo-EM structure of Cas12k-sgRNA binary complex (type V-K CRISPR-associated transposon) | Descriptor: | Cas12k, sgRNA | Authors: | Tenjo-Castano, F, Sofos, N, Stella, S, Molina, R, Pape, T, Lopez-Mendez, B, Stutzke, L.S, Temperini, P, Montoya, G. | Deposit date: | 2022-08-31 | Release date: | 2024-04-10 | Method: | ELECTRON MICROSCOPY (2.87 Å) | Cite: | Structural prediction of the Cas12k interaction with the transposon pre-integration complex To Be Published
|
|
8AXA
| Cryo-EM structure of shCas12k-sgRNA-dsDNA ternary complex (type V-K CRISPR-associated transposon) | Descriptor: | Cas12k, DNA non-target strand, DNA target strand, ... | Authors: | Tenjo-Castano, F, Sofos, N, Stella, S, Fuglsang, A, Pape, T, Mesa, P, Stutzke, L.S, Temperini, P, Montoya, G. | Deposit date: | 2022-08-31 | Release date: | 2024-04-10 | Method: | ELECTRON MICROSCOPY (2.96 Å) | Cite: | Conformational Landscape of the Type V-K CRISPR-associated Transposon Integration Assembly To Be Published
|
|
7ODF
| Structure of the mini-RNA-guided endonuclease CRISPR-Cas_phi3 | Descriptor: | Cas_phi3, DNA (5'-D(P*GP*G)-3'), DNA (5'-D(P*GP*TP*AP*AP*TP*TP*CP*AP*G)-3'), ... | Authors: | Carabias del Rey, A, Fugilsang, A, Temperini, P, Pape, T, Sofos, N, Stella, S, Erledsson, S, Montoya, G. | Deposit date: | 2021-04-29 | Release date: | 2021-07-21 | Last modified: | 2021-08-11 | Method: | ELECTRON MICROSCOPY (2.7 Å) | Cite: | Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3. Nat Commun, 12, 2021
|
|
6S6B
| Type III-B Cmr-beta Cryo-EM structure of the Apo state | Descriptor: | CRISPR-associated RAMP protein, Cmr4 family, Cmr6 family, ... | Authors: | Sofos, N, Montoya, G, Stella, S. | Deposit date: | 2019-07-02 | Release date: | 2020-07-08 | Last modified: | 2020-09-16 | Method: | ELECTRON MICROSCOPY (2.75 Å) | Cite: | Structures of the Cmr-beta Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas. Mol.Cell, 79, 2020
|
|
6S8B
| Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 1 | Descriptor: | CRISPR-associated RAMP protein, Cmr4 family, Cmr6 family, ... | Authors: | Sofos, N, Montoya, G, Stella, S. | Deposit date: | 2019-07-09 | Release date: | 2020-07-08 | Last modified: | 2020-09-16 | Method: | ELECTRON MICROSCOPY (2.41 Å) | Cite: | Structures of the Cmr-beta Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas. Mol.Cell, 79, 2020
|
|
6S91
| Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2 | Descriptor: | CRISPR-associated RAMP protein, Cmr4 family, Cmr6 family, ... | Authors: | Sofos, N, Montoya, G, Stella, S. | Deposit date: | 2019-07-11 | Release date: | 2020-07-08 | Last modified: | 2020-09-16 | Method: | ELECTRON MICROSCOPY (2.68 Å) | Cite: | Structures of the Cmr-beta Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas. Mol.Cell, 79, 2020
|
|
6SIC
| Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA | Descriptor: | CRISPR-associated RAMP protein, Cmr1 family, Cmr4 family, ... | Authors: | Sofos, N, Montoya, G, Stella, S. | Deposit date: | 2019-08-09 | Release date: | 2020-07-08 | Last modified: | 2020-09-16 | Method: | ELECTRON MICROSCOPY (3.52 Å) | Cite: | Structures of the Cmr-beta Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas. Mol.Cell, 79, 2020
|
|
6GTE
| Transient state structure of CRISPR-Cpf1 (Cas12a) I3 conformation | Descriptor: | CRISPR-associated endonuclease Cas12a, DNA (5'-D(P*AP*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3'), DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*G)-3'), ... | Authors: | Montoya, G, Mesa, P, Stella, S. | Deposit date: | 2018-06-18 | Release date: | 2018-12-19 | Last modified: | 2024-05-15 | Method: | ELECTRON MICROSCOPY (4.07 Å) | Cite: | Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity. Cell, 175, 2018
|
|
6GTG
| Transition state structure of Cpf1(Cas12a) I4 conformation | Descriptor: | CRISPR-associated endonuclease Cas12a, DNA (32-MER), DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3'), ... | Authors: | Mesa, P, Montoya, G, Stella, S. | Deposit date: | 2018-06-18 | Release date: | 2018-12-19 | Last modified: | 2024-05-15 | Method: | ELECTRON MICROSCOPY (3.27 Å) | Cite: | Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity. Cell, 175, 2018
|
|
6GTF
| Transient state structure of CRISPR-Cpf1 (Cas12a) I5 conformation | Descriptor: | CRISPR-associated endonuclease Cas12a, DNA (5'-D(P*AP*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3'), DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*A)-3'), ... | Authors: | Montoya, G, Mesa, P, Stella, S. | Deposit date: | 2018-06-18 | Release date: | 2018-12-19 | Last modified: | 2024-05-15 | Method: | ELECTRON MICROSCOPY (3.63 Å) | Cite: | Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity. Cell, 175, 2018
|
|
4AAB
| Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3') | Descriptor: | 10MER DNA 5'-D(*GP*AP*CP*GP*TP*TP*TP*TP*GP*AP)-3', 14MER DNA 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP)-3', DNA ENDONUCLEASE I-CREI, ... | Authors: | Molina, R, Redondo, P, Stella, S, Marenchino, M, D'Abramo, M, Gervasio, F.L, Epinat, J.C, Valton, J, Grizot, S, Duchateau, P, Prieto, J, Montoya, G. | Deposit date: | 2011-12-01 | Release date: | 2012-05-02 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.5 Å) | Cite: | Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage. Nucleic Acids Res., 40, 2012
|
|
7PQ6
| Crystal Structure of the Ring Nuclease 0811 mutant-S12A from Sulfolobus islandicus (Sis0811) | Descriptor: | CRISPR-associated protein, APE2256 family | Authors: | Molina, R, Jensen, A.L.G, Marchena-Hurtado, J, Lopez-Mendez, B, Stella, S, Montoya, G. | Deposit date: | 2021-09-16 | Release date: | 2021-11-24 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.67 Å) | Cite: | Structural basis of cyclic oligoadenylate degradation by ancillary Type III CRISPR-Cas ring nucleases. Nucleic Acids Res., 49, 2021
|
|
7PQ3
| Crystal Structure of the Ring Nuclease 0811 from Sulfolobus islandicus (Sis0811) in complex with its post-catalytic reaction product | Descriptor: | 3'-O-[(R)-{[(2S,3aS,4S,6S,6aS)-6-(6-amino-9H-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2H-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine, CRISPR-associated protein, APE2256 family | Authors: | Molina, R, Jensen, A.L.G, Marchena-Hurtado, J, Lopez-Mendez, B, Stella, S, Montoya, G. | Deposit date: | 2021-09-16 | Release date: | 2021-11-24 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.85 Å) | Cite: | Structural basis of cyclic oligoadenylate degradation by ancillary Type III CRISPR-Cas ring nucleases. Nucleic Acids Res., 49, 2021
|
|
7PQ2
| Crystal Structure of the Ring Nuclease 0811 from Sulfolobus islandicus (Sis0811) in its apo form | Descriptor: | CRISPR-associated protein, APE2256 family, CRISPR Ring Nuclease | Authors: | Molina, R, Jensen, A.L.G, Marchena-Hurtado, J, Lopez-Mendez, B, Stella, S, Montoya, G. | Deposit date: | 2021-09-16 | Release date: | 2021-11-24 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.38 Å) | Cite: | Structural basis of cyclic oligoadenylate degradation by ancillary Type III CRISPR-Cas ring nucleases. Nucleic Acids Res., 49, 2021
|
|
7PQA
| Crystal Structure of the Ring Nuclease 0811 mutant-S12G/K169G from Sulfolobus islandicus (Sis0811) | Descriptor: | CRISPR-associated protein, APE2256 family | Authors: | Molina, R, Jensen, A.L.G, Marchena-Hurtado, J, Lopez-Mendez, B, Stella, S, Montoya, G. | Deposit date: | 2021-09-16 | Release date: | 2021-12-01 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.04 Å) | Cite: | Structural basis of cyclic oligoadenylate degradation by ancillary Type III CRISPR-Cas ring nucleases. Nucleic Acids Res., 49, 2021
|
|
7Z56
| Crystal Structure of the Ring Nuclease 0455 from Sulfolobus islandicus (Sis0455) in its apo form | Descriptor: | CRISPR-associated protein | Authors: | Molina, R, Martin-Garcia, R, Lopez-Mendez, B, Jensen, A.L.G, Marchena-Hurtado, J, Stella, S, Montoya, G. | Deposit date: | 2022-03-08 | Release date: | 2022-11-23 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.27 Å) | Cite: | Molecular basis of cyclic tetra-oligoadenylate processing by small standalone CRISPR-Cas ring nucleases. Nucleic Acids Res., 50, 2022
|
|
4D6N
| THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 10 DAYS INCUBATION IN 5MM MG (STATE 7) | Descriptor: | 5'-D(*DCP*CP*GP*GP*CP*AP*AP*GP*GP*CP)-3', 5'-D(*DCP*GP*CP*GP*CP*CP*GP*GP*AP*AP*CP*TP*TP*DAP*C)-3', 5'-D(*DGP*CP*CP*TP*TP*GP*CP*CP*GP*GP*GP*TP*AP*DAP)-3', ... | Authors: | Molina, R, Stella, S, Redondo, P, Gomez, H, Marcaida, M.J, Orozco, M, Prieto, J, Montoya, G. | Deposit date: | 2014-11-13 | Release date: | 2014-12-17 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.35 Å) | Cite: | Visualizing Phosphodiester-Bond Hydrolysis by an Endonuclease. Nat.Struct.Mol.Biol., 22, 2015
|
|
4D6O
| THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 1H INCUBATION IN 5MM MG (STATE 2) | Descriptor: | 25MER, ACETATE ION, HOMING ENDONUCLEASE I-DMOI, ... | Authors: | Molina, R, Stella, S, Redondo, P, Gomez, H, Marcaida, M.J, Orozco, M, Prieto, J, Montoya, G. | Deposit date: | 2014-11-13 | Release date: | 2014-12-17 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.2 Å) | Cite: | Visualizing Phosphodiester-Bond Hydrolysis by an Endonuclease. Nat.Struct.Mol.Biol., 22, 2015
|
|
4AAF
| Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3') | Descriptor: | 1,2-ETHANEDIOL, 24MER DNA, DNA ENDONUCLEASE I-CREI | Authors: | Molina, R, Redondo, P, Stella, S, Marenchino, M, D'Abramo, M, Gervasio, F.L, Epinat, J.C, Valton, J, Grizot, S, Duchateau, P, Prieto, J, Montoya, G. | Deposit date: | 2011-12-01 | Release date: | 2012-05-02 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.5 Å) | Cite: | Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage. Nucleic Acids Res., 40, 2012
|
|
4AAE
| Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by AGCG from 5' to 3') | Descriptor: | 24MER DNA, DNA ENDONUCLEASE I-CREI | Authors: | Molina, R, Redondo, P, Stella, S, Marenchino, M, D'Abramo, M, Gervasio, F.L, Epinat, J.C, Valton, J, Grizot, S, Duchateau, P, Prieto, J, Montoya, G. | Deposit date: | 2011-12-01 | Release date: | 2012-05-02 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.6 Å) | Cite: | Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage. Nucleic Acids Res., 40, 2012
|
|
4AAG
| Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') | Descriptor: | 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP *GP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3', CALCIUM ION, DNA ENDONUCLEASE I-CREI | Authors: | Molina, R, Redondo, P, Stella, S, Marenchino, M, D'Abramo, M, Gervasio, F, Epinat, J.C, Valton, J, Grizot, S, Duchateau, P, Prieto, J, Montoya, G. | Deposit date: | 2011-12-01 | Release date: | 2012-05-02 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.8 Å) | Cite: | Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage. Nucleic Acids Res., 40, 2012
|
|
4AAD
| Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') | Descriptor: | 24MER DNA, DNA ENDONUCLEASE I-CREI, GLYCEROL | Authors: | Molina, R, Redondo, P, Stella, S, Marenchino, M, D'Abramo, M, Gervasio, F.L, Epinat, J.C, Valton, J, Grizot, S, Duchateau, P, Prieto, J, Montoya, G. | Deposit date: | 2011-12-01 | Release date: | 2012-05-02 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (3.1 Å) | Cite: | Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage. Nucleic Acids Res., 40, 2012
|
|
7Z55
| Crystal Structure of the Ring Nuclease 0455 from Sulfolobus islandicus (Sis0455) in complex with its substrate | Descriptor: | ACETATE ION, CRISPR-associated protein, Cyclic RNA cA4, ... | Authors: | Molina, R, Martin-Garcia, R, Lopez-Mendez, B, Jensen, A.L.G, Marchena-Hurtado, J, Stella, S, Montoya, G. | Deposit date: | 2022-03-08 | Release date: | 2022-11-23 | Last modified: | 2023-11-22 | Method: | X-RAY DIFFRACTION (1.664 Å) | Cite: | Molecular basis of cyclic tetra-oligoadenylate processing by small standalone CRISPR-Cas ring nucleases. Nucleic Acids Res., 50, 2022
|
|