5YCO

Complex structure of PCNA with UHRF2

Summary for 5YCO

• Entry DOI: 10.2210/pdb5yco/pdb
• Descriptor: Proliferating cell nuclear antigen, E3 ubiquitin-protein ligase UHRF2, GLYCEROL, ... (5 entities in total)
• Functional Keywords: complex structure, pcna, uhrf2, dna binding protein
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : P12004 Q96PU4
• Total number of polymer chains: 6
• Total formula weight: 124603.02

Authors

Wu, M.,Chen, W.,Hang, T.,Wang, C.,Zhang, X.,Zang, J. (deposition date: 2017-09-07, release date: 2017-11-15, Last modification date: 2023-11-22)

Primary citation

Chen, W.,Wu, M.,Hang, T.,Wang, C.,Zhang, X.,Zang, J.
Structure insights into the molecular mechanism of the interaction between UHRF2 and PCNA.
Biochem. Biophys. Res. Commun., 494:575-580, 2017

PubMed Abstract: UHRF2 (Ubiquitin-like with PHD and ring finger domains 2) is an E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications and cell cycle regulation by interacting with multiple epigenetic or cell-cycle related proteins. Previous studied have identified PCNA (Proliferating cell nuclear antigen) as an interacting partner of UHRF2 by using the antibody microarray. However, the molecular mechanism and the function of UHRF2-PCNA interaction remains unclear. Here, we report the complex structure of PCNA and the peptide (NEILQTLLDLFFPGYSK) derived from UHRF2 that contains a PIP box. Structural analysis combined with mutagenesis experiments provide the molecular basis for the recognition of UHRF2 by PCNA via PIP-box.

PubMed: 28951215
DOI: 10.1016/j.bbrc.2017.09.102

Experimental method

X-RAY DIFFRACTION (2.199 Å)