5IY4

Crystal structure of human PCNA in complex with the PIP box of DVC1

Summary for 5IY4

• Entry DOI: 10.2210/pdb5iy4/pdb
• Descriptor: Proliferating cell nuclear antigen, DVC1 PIP box (2 entities in total)
• Functional Keywords: pcna, pip box, dvc1, dna binding protein
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : P12004
• Total number of polymer chains: 6
• Total formula weight: 95568.07

Authors

Jiang, T.,Xu, M.,Wang, Y. (deposition date: 2016-03-24, release date: 2016-06-08, Last modification date: 2024-10-30)

Primary citation

Wang, Y.,Xu, M.,Jiang, T.
Crystal structure of human PCNA in complex with the PIP box of DVC1
Biochem.Biophys.Res.Commun., 474:264-270, 2016

PubMed Abstract: In higher eukaryotes, DVC1 (SPRTN, Spartan or C1orf124) is implicated in the translesion synthesis (TLS) pathway. DVC1 localizes to sites of DNA damage, binds to the proliferating cell nuclear antigen (PCNA) via its conserved PCNA-interacting motif (PIP box), and associates with ubiquitin selective segregase p97 and other factors, thus regulating translesion synthesis polymerases. Here, we report the crystal structure of human PCNA in complex with a peptide ((321)SNSHQNVLSNYFPRVS(336)) derived from human DVC1 that contains a unique YF type PIP box. Structural analysis reveals the detailed PIP box-PCNA interaction. Interestingly, substitution of Y331 with Phe severely reduces its PCNA binding affinity. These findings offer new insights into the determinants of PIP box for PCNA binding.

PubMed: 27084448
DOI: 10.1016/j.bbrc.2016.04.053

Experimental method

X-RAY DIFFRACTION (2.945 Å)