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4EJ1

Binding of Nb113 camelid antibody fragment with the binary DHFR:folate complex

Summary for 4EJ1

Related4EIG 4EIZ 
DescriptorDihydrofolate reductase (E.C.1.5.1.3), Nb113 camelid antibody fragment
Functional KeywordsFOLATE, Oxidoreductase-Immune system complex,Oxidoreductase/Immune system
Biological sourceEscherichia coli
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Total number of polymer chains4
Total molecular weight67122.35
Authors
Oyen, D.,Srinivasan, V. (deposition date: 2012-04-06, release date: 2013-04-24, modification date: 2013-10-30)
Primary citation
Oyen, D.,Wechselberger, R.,Srinivasan, V.,Steyaert, J.,Barlow, J.N.
Mechanistic analysis of allosteric and non-allosteric effects arising from nanobody binding to two epitopes of the dihyrofolate reductase of Escherichia coli.
Biochim.Biophys.Acta, 1834:2147-2157, 2013
PubMed: 23911607
DOI: 10.1016/j.bbapap.2013.07.010
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.75 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.221401.7%5.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolutionDownload full validation report

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entries available on 2014-11-26
00:00 UTC / 09:00 JST