3UGO

Crystal structure of RNA-polymerase sigma subunit domain 2 complexed with -10 promoter element ssDNA oligo (TACAAT)

Summary for 3UGO

Related3UGP
DescriptorRNA polymerase sigma factor/DNA complex
Functional Keywordsprotein-dna complex, bacterial promoter opening, g-quartet, g-quadruplex, dna binding, transcription-dna complex, transcription/dna
Biological sourceThermus aquaticus
Total number of polymer chains3
Total molecular weight35060.68
Authors
Feklistov, A.,Darst, S.A. (deposition date: 2011-11-02, release date: 2011-12-07, modification date: 2012-03-07)
Primary citation
Feklistov, A.,Darst, S.A.
Structural basis for promoter-10 element recognition by the bacterial RNA polymerase sigma subunit.
Cell(Cambridge,Mass.), 147:1257-1269, 2011
PubMed: 22136875
DOI: 10.1016/j.cell.2011.10.041
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.096 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.230602.0%8.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 3ugo
no rotation
Molmil generated image of 3ugo
rotated about x axis by 90°
Molmil generated image of 3ugo
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 3ugo
no rotation
Molmil generated image of 3ugo
rotated about x axis by 90°
Molmil generated image of 3ugo
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (3ugo.pdb1.gz [38.64 KB])
Coordinate files for Biological unit (3ugo.pdb2.gz [5.47 KB])
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