2ADJ

Crystal structure of monoclonal anti-CD4 antibody Q425 in complex with Calcium

> Summary

Summary for 2ADJ

Related2ADG 2ADI
DescriptorQ425 Fab Light chain, Q425 Fab Heavy chain
Functional Keywordsanti-cd4, interfacial metal, antibody recognition, immune system
Biological sourceMus musculus (house mouse)
Total number of polymer chains2
Total molecular weight47691.15
Authors
Zhou, T.,Hamer, D.H.,Hendrickson, W.A.,Sattentau, Q.J.,Kwong, P.D. (deposition date: 2005-07-20, release date: 2005-09-20, modification date: 2009-02-24)
Primary citation
Zhou, T.,Hamer, D.H.,Hendrickson, W.A.,Sattentau, Q.J.,Kwong, P.D.
Interfacial metal and antibody recognition.
Proc.Natl.Acad.Sci.Usa, 102:14575-14580, 2005
PubMed: 16195378
DOI: 10.1073/pnas.0507267102
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.9 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.296282.9%8.8%3.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 2adj
no rotation
Molmil generated image of 2adj
rotated about x axis by 90°
Molmil generated image of 2adj
rotated about y axis by 90°

> Structural details

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains2
Total molecular weight47651.1
Non-Polymers*Number of molecules1
Total molecular weight40.1
All*Total molecular weight47691.1
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (2.9 Å)

Cell axes97.22497.224110.976
Cell angles90.0090.0090.00
SpacegroupP 43 21 2
Resolution limits9.98 - 2.90
the highest resolution shell value3.080 - 2.900
R-factor0.213
R-work0.21300
the highest resolution shell value0.318
R-free0.30000
the highest resolution shell value0.458
RMSD bond length0.008
RMSD bond angle1.500

Data Collection Statistics

Resolution limits10.00 - 2.90
the highest resolution shell value -
Number of reflections10984
Completeness92.0
Redundancy5.6
I/sigma(I)2

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, HANGING DROP5.5298

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
A0005886cellular_componentplasma membrane
A0003823molecular_functionantigen binding
A0030183biological_processB cell differentiation
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
AC14BINDING SITE FOR RESIDUE CA A 3000
ChainResidue
AASP32
AGLU50
ASER91
BASN105

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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
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Functional Information from PROSITE/UniProt

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Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails
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Catalytic Information from CSA

site_idNumber of ResiduesDetails
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Catalytic Information from CATRES

site_idNumber of ResiduesDetails

> Sequence Neighbor

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