1DRZ
U1A SPLICEOSOMAL PROTEIN/HEPATITIS DELTA VIRUS GENOMIC RIBOZYME COMPLEX
Summary for 1DRZ
| Entry DOI | 10.2210/pdb1drz/pdb |
| Descriptor | RNA (HEPATITIS DELTA VIRUS GENOMIC RIBOZYME), PROTEIN (U1 SMALL RIBONUCLEOPROTEIN A), MAGNESIUM ION, ... (5 entities in total) |
| Functional Keywords | catalytic rna, ribozyme, rna-binding protein, u1a, hdv, rna binding protein-rna complex, rna binding protein/rna |
| Biological source | Hepatitis delta virus More |
| Total number of polymer chains | 2 |
| Total formula weight | 34841.53 |
| Authors | Ferre-D'Amare, A.R.,Zhou, K.,Doudna, J.A. (deposition date: 1998-09-01, release date: 1999-02-16, Last modification date: 2024-10-09) |
| Primary citation | Ferre-D'Amare, A.R.,Zhou, K.,Doudna, J.A. Crystal structure of a hepatitis delta virus ribozyme. Nature, 395:567-574, 1998 Cited by PubMed Abstract: The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes. PubMed: 9783582DOI: 10.1038/26912 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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