8EKG
MHETase variant Thr159Val, Met192Tyr, Tyr252Phe, Tyr503Trp
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... | Mono(2-hydroxyethyl) terephthalate hydrolase | polymer | 592 | 62372.4 | 6 | UniProt (A0A0K8P8E7) Pfam (PF07519) In PDB | Ideonella sakaiensis | MHET hydrolase,MHETase |
| 2 | F, A, B, C, D... | CALCIUM ION | non-polymer | 40.1 | 6 | Chemie (CA) | |||
| 3 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 4 | E, A, B, C, D | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
| 5 | B, E | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 6 | water | water | 18.0 | 403 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 20 - 603 (UniProt: A0A0K8P8E7)
| PDB | External Database | Details |
|---|---|---|
| Met 12 | - | initiating methionine |
| Glu 13 | - | expression tag |
| Asn 14 | - | expression tag |
| Leu 15 | - | expression tag |
| Tyr 16 | - | expression tag |
| Phe 17 | - | expression tag |
| Gln 18 | - | expression tag |
| Gly 19 | - | expression tag |
| Val 159 | Thr 159 | engineered mutation |
| Tyr 192 | Met 192 | engineered mutation |
| Phe 252 | Tyr 252 | engineered mutation |
| Trp 503 | Tyr 503 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 374234.5 | |
| Non-Polymers* | Number of molecules | 22 |
| Total formula weight | 1413.6 | |
| All* | Total formula weight | 375648.1 |
