8CIP

Crystal structure of transketolase from Geobacillus stearothermophilus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Transketolase	polymer	681	73507.2	4	UniProt (A0A0I9QGZ2) Pfam (PF00456) Pfam (PF02779) Pfam (PF22613)	Geobacillus stearothermophilus	TK
2	AA, BA, CA, E, EA... (C, A, D, B)	1,2-ETHANEDIOL	non-polymer		62.1	19	Chemie (EDO)
3	DA, KA, L (C, D, A)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
4	FA, LA, M, U (C, D, A, B)	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
5	GA, MA, N, V (C, D, A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	HA, O, P, Q, W (C, A, B)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
7	NA, OA, PA, QA (A, B, C, D)	water	water		18.0	600	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 668 (UniProt: A0A0I9QGZ2)

PDB	External Database	Details
Met -12	-	initiating methionine
Ala -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Ser -4	-	expression tag
Ala -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Leu 0	-	expression tag
Phe 397	Leu 397	conflict
Gly 399	Asp 399	conflict
Gln 479	His 479	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	294028.7
Non-Polymers*	Number of molecules	35
	Total formula weight	3332.2
All*	Total formula weight	297360.9

*Water molecules are not included.