7XU5

Structure of SARS-CoV-2 D614G Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), Closed Conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A, C	Spike glycoprotein	polymer	1127	124669.2	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Severe acute respiratory syndrome coronavirus 2	S glycoprotein,E2,Peplomer protein
2	K, M, Z, b, o...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	6	In PDB GlyTouCan (G42666HT)
3	C, B, A	BILIVERDINE IX ALPHA	non-polymer		582.6	3	Chemie (BLA)
4	A, C, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	30	Chemie (NAG)

Sequence modifications

B, A, C: 14 - 1140 (UniProt: P0DTC2)

PDB	External Database	Details
Glu 10	-	expression tag
Thr 11	-	expression tag
Gly 12	-	expression tag
Thr 13	-	expression tag
Cys 427	Asp 427	engineered mutation
Gly 614	Asp 614	engineered mutation
-	Pro 681	deletion
-	Arg 682	deletion
-	Arg 683	deletion
-	Ala 684	deletion
Cys 987	Val 987	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	374007.6
Branched	Number of molecules	6
	Total formula weight	2546.4
Non-Polymers*	Number of molecules	33
	Total formula weight	8384.2
All*	Total formula weight	384938.2

*Water molecules are not included.