7AHK

Crystal structure of the outward-facing state of the substrate-free Na+-only bound glutamate transporter homolog GltPh

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glutamate transporter homolog	polymer	425	44843.1	1	UniProt (O59010) Pfam (PF00375) In PDB	Pyrococcus horikoshii	Glt(Ph),Sodium-aspartate symporter Glt(Ph),Sodium-dependent aspartate transporter
2	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
3	A	octyl beta-D-glucopyranoside	non-polymer		292.4	1	Chemie (BOG)
4	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5	A	EICOSANE	non-polymer		282.5	12	Chemie (LFA)
6	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7	A	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	2	Chemie (OLC)
8		water	water		18.0	24	Chemie (HOH)

Sequence modifications

A: 1 - 417 (UniProt: O59010)

PDB	External Database	Details
His 37	Asp 37	engineered mutation
His 40	Lys 40	engineered mutation
His 125	Lys 125	engineered mutation
His 132	Lys 132	engineered mutation
His 223	Lys 223	engineered mutation
His 264	Lys 264	engineered mutation
His 368	Glu 368	engineered mutation
Thr 418	-	expression tag
Leu 419	-	expression tag
Val 420	-	expression tag
Pro 421	-	expression tag
Arg 422	-	expression tag
Gly 423	-	expression tag
Ser 424	-	expression tag
Gly 425	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44843.1
Non-Polymers*	Number of molecules	20
	Total formula weight	4721.2
All*	Total formula weight	49564.3

*Water molecules are not included.