6KDO
HIV-1 reverse transcriptase with Q151M/Y115F/F116Y/M184V/F160M:DNA:lamivudine 5'-triphosphate ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C | HIV-1 reverse transcriptase p66 subunit | polymer | 557 | 63985.3 | 2 | UniProt (D3XFN5) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) UniProt (by SIFTS) (P12497) In PDB | Human immunodeficiency virus 1 | |
| 2 | B, D | HIV-1 RT p51 subunit | polymer | 444 | 51861.5 | 2 | UniProt (P12497) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB | Human immunodeficiency virus type 1 | HIV-1 reverse transcriptase p51 subunit, p51 RT |
| 3 | E, F | DNA/RNA (38-MER) | polymer | 38 | 11764.5 | 2 | synthetic construct | ||
| 4 | G | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900003) | sucrose | ||
| 5 | A, B, C, D | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 6 | E, C | Lamivudine Triphosphate | non-polymer | 469.2 | 2 | Chemie (1RZ) | |||
| 7 | water | water | 18.0 | 192 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 555 (UniProt: D3XFN5)
B, D: 1 - 428 (UniProt: P12497)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Val 0 | - | expression tag |
| Phe 115 | Tyr 214 | engineered mutation |
| Tyr 116 | Phe 215 | engineered mutation |
| Met 151 | Gln 250 | engineered mutation |
| Met 160 | Phe 259 | engineered mutation |
| Ser 162 | Cys 261 | engineered mutation |
| Val 184 | Met 283 | engineered mutation |
| Ser 280 | Cys 379 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | expression tag |
| Ala -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 162 | Cys 749 | engineered mutation |
| Ser 280 | Cys 867 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 255222.8 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1306.8 | |
| All* | Total formula weight | 256871.9 |
