5C7R

Revealing surface waters on an antifreeze protein by fusion protein crystallography

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Fusion protein of Maltose-binding periplasmic protein and Type-3 ice-structuring protein HPLC 12	polymer	444	48512.2	2	UniProt (P0AEY0) UniProt (P19614) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli O157:H7	MBP,MMBP,Maltodextrin-binding protein,Antifreeze protein QAE(HPLC 12),ISP type III HPLC 12
2	C, D	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	2	In PDB BIRD (PRD_900009) GlyTouCan (G96370VA)		alpha-maltotriose
3	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4		water	water		18.0	540	Chemie (HOH)

Sequence modifications

A, B: 1 - 358 (UniProt: P0AEY0)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 359	-	linker
Ala 360	-	linker
Leu 361	-	linker
Ala 362	-	linker
Ala 363	-	linker
Ala 364	-	linker
Gln 365	-	linker
Thr 366	-	linker
Asn 367	-	linker
Ala 368	-	linker
Ala 369	-	linker
Ala 370	-	linker

A, B: 371 - 433 (UniProt: P19614)

PDB	External Database	Details
Ala 434	-	expression tag
Ala 435	-	expression tag
Leu 436	-	expression tag
Glu 437	-	expression tag
His 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97024.3
Branched	Number of molecules	2
	Total formula weight	1008.9
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	98225.3

*Water molecules are not included.