1B2M
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THREE-DIMENSIONAL STRUCTURE OF RIBONULCEASE T1 COMPLEXED WITH AN ISOSTERIC PHOSPHONATE ANALOGUE OF GPU: ALTERNATE SUBSTRATE BINDING MODES AND CATALYSIS.
Descriptor:ENDORIBONUCLEASE T1/DINUCLEOTIDE COMPLEX
Authors:Arni, R.K., Watanabe, L., Ward, R.J., Kreitman, R.J., Kumar, K., Walz jr., F.G.
Deposit date:1998-11-27
Release date:1999-03-25
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (2 Å)
Cite:Three-dimensional structure of ribonuclease T1 complexed with an isosteric phosphonate substrate analogue of GpU: alternate substrate binding modes and catalysis.
Biochemistry, 38, 1999
1BIR
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RIBONUCLEASE T1, PHE 100 TO ALA MUTANT COMPLEXED WITH 2' GMP
Descriptor:RIBONUCLEASE T1, GUANOSINE-2'-MONOPHOSPHATE
Authors:Doumen, J., Gonciarz, M., Zegers, I., Loris, R., Wyns, L., Steyaert, J.
Deposit date:1996-01-04
Release date:1996-08-17
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:A catalytic function for the structurally conserved residue Phe 100 of ribonuclease T1.
Protein Sci., 5, 1996
1BU4
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RIBONUCLEASE 1 COMPLEX WITH 2'GMP
Descriptor:RIBONUCLEASE T1, GUANOSINE-2'-MONOPHOSPHATE
Authors:Loris, R., Devos, S., Langhorst, U., Decanniere, K., Bouckaert, J., Maes, D., Transue, T.R., Steyaert, J.
Deposit date:1998-09-11
Release date:1999-02-16
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Conserved water molecules in a large family of microbial ribonucleases.
Proteins, 36, 1999
1BVI
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RIBONUCLEASE T1 (WILDTYPE) COMPLEXED WITH 2'GMP
Descriptor:PROTEIN (RIBONUCLEASE T1) (3.1.27.3)
Authors:Langhorst, U., Loris, R., Denisov, V.P., Doumen, J., Roose, P., Maes, D., Halle, B., Steyaert, J.
Deposit date:1998-09-15
Release date:1998-09-23
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Dissection of the structural and functional role of a conserved hydration site in RNase T1.
Protein Sci., 8, 1999
1CH0
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RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT
Descriptor:RIBONUCLEASE T1 (E.C.3.1.27.3) variant + 2'-GMP
Authors:Hoeschler, K., Hoier, H., Orth, P., Hubner, B., Saenger, W., Hahn, U.
Deposit date:1999-03-30
Release date:1999-12-22
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Structural analysis of an RNase T1 variant with an altered guanine binding segment.
J.Mol.Biol., 294, 1999
1DET
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RIBONUCLEASE T1 CARBOXYMETHYLATED AT GLU 58 IN COMPLEX WITH 2'GMP
Descriptor:RIBONUCLEASE T1, GUANOSINE-2'-MONOPHOSPHATE, CARBOXYMETHYL GROUP
Authors:Ishikawa, K., Suzuki, E., Tanokura, M., Takahashi, K.
Deposit date:1996-02-20
Release date:1996-07-11
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Crystal structure of ribonuclease T1 carboxymethylated at Glu58 in complex with 2'-GMP.
Biochemistry, 35, 1996
1FYS
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RIBONUCLEASE T1 V16C MUTANT
Descriptor:GUANYL-SPECIFIC RIBONUCLEASE T1 (E.C.3.1.27.3)
Authors:De Vos, S., Loris, R., Steyaert, J.
Deposit date:2000-10-03
Release date:2000-10-25
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (2 Å)
Cite:Hydrophobic core manipulations in ribonuclease T1.
Biochemistry, 40, 2001
1FZU
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RNASE T1 V78A MUTANT
Descriptor:GUANYL-SPECIFIC RIBONUCLEASE T1 (E.C.3.1.27.3)
Authors:De Vos, S., Loris, R., Steyaert, J.
Deposit date:2000-10-04
Release date:2000-10-25
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Hydrophobic core manipulations in ribonuclease T1.
Biochemistry, 40, 2001
1G02
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RIBONUCLEASE T1 V16S MUTANT
Descriptor:GUANYL-SPECIFIC RIBONUCLEASE T1 (E.C.3.1.27.3)
Authors:De Vos, S., Loris, R., Steyaert, J.
Deposit date:2000-10-05
Release date:2000-10-25
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.86 Å)
Cite:Hydrophobic core manipulations in ribonuclease T1.
Biochemistry, 40, 2001
1GSP
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RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY
Descriptor:RIBONUCLEASE T1, GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE
Authors:Zegers, I., Wyns, L.
Deposit date:1997-11-28
Release date:1998-02-25
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography.
Nat.Struct.Biol., 5, 1998
1HYF
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RIBONUCLEASE T1 V16A MUTANT IN COMPLEX WITH SR2+
Descriptor:GUANYL-SPECIFIC RIBONUCLEASE T1(E.C.3.1.27.3), GUANOSINE-2'-MONOPHOSPHATE
Authors:De Swarte, J., De Vos, S., Langhorst, U., Steyaert, J., Loris, R.
Deposit date:2001-01-19
Release date:2001-02-14
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution.
Eur.J.Biochem., 268, 2001
1HZ1
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RIBONUCLEASE T1 V16A MUTANT IN COMPLEX WITH MG2+
Descriptor:RIBONUCLEASE T1 (E.C.3.1.27.3)
Authors:De Swarte, J., De Vos, S., Langhorst, U., Steyaert, J., Loris, R.
Deposit date:2001-01-23
Release date:2001-01-31
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution.
Eur.J.Biochem., 268, 2001
1I0V
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RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM I CRYSTAL)
Descriptor:GUANYL-SPECIFIC RIBONUCLEASE T1 (E.C.3.1.27.3)
Authors:De Swarte, J., De Vos, S., Langhorst, U., Steyaert, J., Loris, R.
Deposit date:2001-01-30
Release date:2001-02-14
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.234 Å)
Cite:The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution.
Eur.J.Biochem., 268, 2001
1I0X
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RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL)
Descriptor:GUANYL-SPECIFIC RIBONUCLEASE T1 (E.C.3.1.27.3)
Authors:De Swarte, J., De Vos, S., Langhorst, U., Steyaert, J., Loris, R.
Deposit date:2001-01-30
Release date:2001-02-14
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.65 Å)
Cite:The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution.
Eur.J.Biochem., 268, 2001
1I2E
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RIBONUCLEASE T1 V16A MUTANT, FORM I
Descriptor:GUANYL-SPECIFIC Ribonuclease T1 (E.C.3.1.27.3)
Authors:De Vos, S., Backmann, J., Steyaert, J., Loris, R.
Deposit date:2001-02-09
Release date:2001-03-07
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Hydrophobic core manipulations in ribonuclease T1
Biochemistry, 40, 2001
1I2F
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RIBONUCLEASE T1 V16A MUTANT, FORM II
Descriptor:GUANYL-SPECIFIC Ribonuclease T1 (E.C.3.1.27.3)
Authors:De Vos, S., Backmann, J., Steyaert, J., Loris, R.
Deposit date:2001-02-09
Release date:2001-03-07
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.95 Å)
Cite:Hydrophobic core manipulations in ribonuclease T1
Biochemistry, 40, 2001
1I2G
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RIBONUCLEASE T1 V16T MUTANT
Descriptor:GUANYL-SPECIFIC Ribonuclease T1 (E.C.3.1.27.3)
Authors:De Vos, S., Backmann, J., Steyaert, J., Loris, R.
Deposit date:2001-02-09
Release date:2001-03-07
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.85 Å)
Cite:Hydrophobic core manipulations in ribonuclease T1
Biochemistry, 40, 2001
1I3F
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RIBONUCLEASE T1 V89S MUTANT
Descriptor:GUANYL-SPECIFIC Ribonuclease T1 (E.C.3.1.27.3)
Authors:De Vos, S., Backmann, J., Steyaert, J., Loris, R.
Deposit date:2001-02-15
Release date:2001-03-07
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (2.35 Å)
Cite:Hydrophobic core manipulations in ribonuclease T1
Biochemistry, 40, 2001
1I3I
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RIBONUCLEASE T1 V78T MUTANT
Descriptor:GUANYL-SPECIFIC Ribonuclease T1 (E.C.3.1.27.3)
Authors:De Vos, S., Backmann, J., Steyaert, J., Loris, R.
Deposit date:2001-02-15
Release date:2001-03-07
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.76 Å)
Cite:Hydrophobic core manipulations in ribonuclease T1
Biochemistry, 40, 2001
1IYY
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NMR STRUCTURE OF GLN25-RIBONUCLEASE T1, 24 STRUCTURES
Descriptor:RIBONUCLEASE T1 (E.C.3.1.27.3)
Authors:Hatano, K., Kojima, M., Suzuki, E., Tanokura, M., Takahashi, K.
Deposit date:2002-09-12
Release date:2003-10-07
Modification date:2009-02-24
Method:SOLUTION NMR
Cite:Determination of the NMR structure of Gln25-ribonuclease T1
Biol.Chem., 384, 2003
1LOV
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X-RAY STRUCTURE OF THE E58A MUTANT OF RIBONUCLEASE T1 COMPLEXED WITH 3'-GUANOSINE MONOPHOSPHATE
Descriptor:Guanyl-specific ribonuclease T1 (E.C.3.1.27.3)
Authors:Mignon, P., Steyaert, J., Loris, R., Geerlings, P., Loverix, S.
Deposit date:2002-05-07
Release date:2002-08-21
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.55 Å)
Cite:A nucleophile activation dyad in ribonucleases. A combined X-ray crystallographic/ab initio quantum chemical study
J.Biol.Chem., 277, 2002
1LOW
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X-RAY STRUCTURE OF THE H40A MUTANT OF RIBONUCLEASE T1 COMPLEXED WITH 3'-GUANOSINE MONOPHOSPHATE
Descriptor:Guanyl-specific ribonuclease T1 (E.C.3.1.27.3)
Authors:Mignon, P., Steyaert, J., Loris, R., Geerlings, P., Loverix, S.
Deposit date:2002-05-07
Release date:2002-08-21
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:A nucleophile activation dyad in ribonucleases. A combined X-ray crystallographic/ab initio quantum chemical study
J.Biol.Chem., 277, 2002
1LOY
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X-RAY STRUCTURE OF THE H40A/E58A MUTANT OF RIBONUCLEASE T1 COMPLEXED WITH 3'-GUANOSINE MONOPHOSPHATE
Descriptor:Guanyl-specific ribonuclease T1 (E.C.3.1.27.3)
Authors:Mignon, P., Steyaert, J., Loris, R., Geerlings, P., Loverix, S.
Deposit date:2002-05-07
Release date:2002-08-21
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.55 Å)
Cite:A nucleophile activation dyad in ribonucleases. A combined X-ray crystallographic/ab initio quantum chemical study
J.Biol.Chem., 277, 2002
1LRA
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CRYSTALLOGRAPHIC STUDY OF GLU 58 ALA RNASE T1(ASTERISK)2'-GUANOSINE MONOPHOSPHATE AT 1.9 ANGSTROMS RESOLUTION
Descriptor:RIBONUCLEASE T1 (RNASE T1) (E.C.3.1.27.3) MUTANT WITH GLU 58 REPLACED BY ALA (E58A) COMPLEX WITH 2'-GUANYLIC ACID (2'-GMP)
Authors:Pletinckx, J., Steyaert, J., Choe, H.-W., Heinemann, U., Wyns, L.
Deposit date:1993-10-01
Release date:1994-01-31
Modification date:2011-07-13
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Crystallographic study of Glu58Ala RNase T1 x 2'-guanosine monophosphate at 1.9-A resolution.
Biochemistry, 33, 1994
1Q9E
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RNASE T1 VARIANT WITH ADENINE SPECIFICITY
Descriptor:Guanyl-specific ribonuclease T1 precursor (E.C.3.1.27.3)
Authors:Czaja, R., Struhalla, M., Hoeschler, K., Saenger, W., Straeter, N., Hahn, U.
Deposit date:2003-08-25
Release date:2004-03-23
Modification date:2009-02-24
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:RNase T1 Variant RV Cleaves Single-Stranded RNA after Purines Due to Specific Recognition by the Asn46 Side Chain Amide.
Biochemistry, 43, 2004
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