[English] 日本語
Yorodumi- PDB-5fwh: N-terminal FHA domain from EssC a component of the bacterial Type... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5fwh | ||||||
---|---|---|---|---|---|---|---|
Title | N-terminal FHA domain from EssC a component of the bacterial Type VII secretion apparatus | ||||||
Components | ESSC | ||||||
Keywords | STRUCTURAL PROTEIN / ATPASE / ESX-1 / FORKHEAD-ASSOCIATED DOMAIN / FTSK/SPOIIIE | ||||||
Function / homology | Function and homology information | ||||||
Biological species | GEOBACILLUS THERMODENITRIFICANS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.06 Å | ||||||
Authors | Zoltner, M. / Ng, W.M.A.V. / Money, J.J. / Fyfe, P.K. / Kneuper, H. / Palmer, T. / Hunter, W.N. | ||||||
Citation | Journal: Biochem.J. / Year: 2016 Title: Essc: Domain Structures Inform on the Elusive Translocation Channel in the Type Vii Secretion System. Authors: Zoltner, M. / Ng, W.M.A.V. / Money, J.J. / Fyfe, P.K. / Kneuper, H. / Palmer, T. / Hunter, W.N. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 5fwh.cif.gz | 84.3 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb5fwh.ent.gz | 69.6 KB | Display | PDB format |
PDBx/mmJSON format | 5fwh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fw/5fwh ftp://data.pdbj.org/pub/pdb/validation_reports/fw/5fwh | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 22260.387 Da / Num. of mol.: 1 / Fragment: FHA DOMAIN, RESIDUES 1-198 Source method: isolated from a genetically manipulated source Source: (gene. exp.) GEOBACILLUS THERMODENITRIFICANS (bacteria) Plasmid: PET27B / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A4IKE7 |
---|---|
#2: Water | ChemComp-HOH / |
Sequence details | RESIDUES 1-196 ONLY |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 42 % / Description: NONE |
---|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.92 |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 7, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 2.06→29.5 Å / Num. obs: 12092 / % possible obs: 99.1 % / Observed criterion σ(I): 0 / Redundancy: 11.6 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 27.3 |
Reflection shell | Resolution: 2.06→2.17 Å / Redundancy: 7.1 % / Rmerge(I) obs: 0.45 / Mean I/σ(I) obs: 3.6 / % possible all: 94.4 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: SAD Starting model: NONE Resolution: 2.06→47.08 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.915 / SU B: 18.873 / SU ML: 0.23 / Cross valid method: THROUGHOUT / ESU R: 0.263 / ESU R Free: 0.239 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.06→47.08 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
|