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Yorodumi- PDB-5dfx: Structure of the parental state of GAF3 from Slr1393 of Synechocy... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5dfx | ||||||
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Title | Structure of the parental state of GAF3 from Slr1393 of Synechocystis sp. PCC6803 (in vivo assembled protein/chromophore) | ||||||
Components | Histidine kinase | ||||||
Keywords | TRANSFERASE / cyanobacteriochrome / phycocyanobilin chromophore / photochromicity / bilin-binding GAF domain / phytochrome-related protein | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Synechocystis sp. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å | ||||||
Authors | Xu, X.-L. / Zhao, K.-H. / Gaertner, W. / Hoeppner, A. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2020 Title: Structural elements regulating the photochromicity in a cyanobacteriochrome Authors: Xu, X.-L. / Hoeppner, A. / Wiebeler, C. / Zhao, K.-H. / Schapiro, I. / Gaertner, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5dfx.cif.gz | 51 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5dfx.ent.gz | 37.9 KB | Display | PDB format |
PDBx/mmJSON format | 5dfx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/df/5dfx ftp://data.pdbj.org/pub/pdb/validation_reports/df/5dfx | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 24856.637 Da / Num. of mol.: 1 / Fragment: residues 441-597 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechocystis sp. (strain PCC 6803 / Kazusa) (bacteria) Gene: slr1393 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P73184, histidine kinase |
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#2: Chemical | ChemComp-CYC / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.34 % |
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Crystal grow | Temperature: 285 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.02 M Tris pH 7.0, 0.1 M sodium chloride, 7.7 % (w/v) PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.97916 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 10, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97916 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→19.96 Å / Num. obs: 22947 / % possible obs: 99.7 % / Redundancy: 6.63 % / Rmerge(I) obs: 0.056 / Net I/σ(I): 19.19 |
Reflection shell | Resolution: 1.8→1.91 Å / Redundancy: 6.52 % / Rmerge(I) obs: 0.301 / Mean I/σ(I) obs: 5.42 / % possible all: 99 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.8→19.96 Å / Cor.coef. Fo:Fc: 0.967 / Cor.coef. Fo:Fc free: 0.948 / SU B: 2.063 / SU ML: 0.064 / Cross valid method: THROUGHOUT / ESU R: 0.095 / ESU R Free: 0.097 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.946 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→19.96 Å
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Refine LS restraints |
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