Mass: 18.015 Da / Num. of mol.: 127 / Source method: isolated from a natural source / Formula: H2O
Compound details
BINDS TO ACTIVATED (PHOSPHORYLATED) PROTEIN-TYR KINASES, THROUGH ITS SH2 DOMAIN, AND ACTS AS AN ...BINDS TO ACTIVATED (PHOSPHORYLATED) PROTEIN-TYR KINASES, THROUGH ITS SH2 DOMAIN, AND ACTS AS AN ADAPTER, MEDIATING THE ASSOCIATION OF THE P110 CATALYTIC UNIT TO THE PLASMA MEMBRANE. NECESSARY FOR THE INSULIN-STIMULATED INCREASE IN GLUCOSE UPTAKE AND GLYCOGEN SYNTHESIS IN INSULIN-SENSITIVE TISSUES.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 3.4 Å3/Da / Density % sol: 64 %
Crystal grow
Details: 20% PEG4000, 50MM MGCL2, 50MM TRIS PH7.5
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Data collection
Diffraction
Mean temperature: 113 K
Diffraction source
Source: ROTATING ANODE / Wavelength: 0
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0 Å / Relative weight: 1
Reflection
Resolution: 1.89→20 Å / Num. obs: 14679 / % possible obs: 95.1 % / Rmerge(I) obs: 0.05
Reflection shell
Resolution: 1.9→2 Å / Rmerge(I) obs: 0.19 / Mean I/σ(I) obs: 3.56 / % possible all: 75
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Processing
Software
Name: X-PLOR / Classification: refinement
Refinement
Method to determine structure: OTHER / Resolution: 1.89→15 Å / Rfactor Rfree: 0.289 / Rfactor Rwork: 0.1918 / Rfactor obs: 0.1918 / Cross valid method: THROUGHOUT / σ(F): 0 Details: THIS COODINATES FILE WAS DEPOSITED 10 YEARS AFTER THE PUBLICATION OF THE WORK. SOME OF THE DATA ARE MISSING AND ARE SHOWN AS '00000'
Displacement parameters
Biso mean: 28.86 Å2
Refinement step
Cycle: LAST / Resolution: 1.89→15 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
904
0
0
127
1031
Refine LS restraints
Refine-ID
Type
Dev ideal
X-RAY DIFFRACTION
x_bond_d
0.011
X-RAY DIFFRACTION
x_bond_d_na
X-RAY DIFFRACTION
x_bond_d_prot
X-RAY DIFFRACTION
x_angle_d
X-RAY DIFFRACTION
x_angle_d_na
X-RAY DIFFRACTION
x_angle_d_prot
X-RAY DIFFRACTION
x_angle_deg
1.671
X-RAY DIFFRACTION
x_angle_deg_na
X-RAY DIFFRACTION
x_angle_deg_prot
X-RAY DIFFRACTION
x_dihedral_angle_d
X-RAY DIFFRACTION
x_dihedral_angle_d_na
X-RAY DIFFRACTION
x_dihedral_angle_d_prot
X-RAY DIFFRACTION
x_improper_angle_d
X-RAY DIFFRACTION
x_improper_angle_d_na
X-RAY DIFFRACTION
x_improper_angle_d_prot
X-RAY DIFFRACTION
x_mcbond_it
X-RAY DIFFRACTION
x_mcangle_it
X-RAY DIFFRACTION
x_scbond_it
X-RAY DIFFRACTION
x_scangle_it
+
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