Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria.
Journal, issue, pages	Nat Commun, Vol. 12, Issue 1, Page 120, Year 2021
Publish date	Jan 5, 2021
Authors	Alexander Mühleip / Rasmus Kock Flygaard / Jana Ovciarikova / Alice Lacombe / Paula Fernandes / Lilach Sheiner / Alexey Amunts /
PubMed Abstract	Mitochondrial ATP synthase plays a key role in inducing membrane curvature to establish cristae. In Apicomplexa causing diseases such as malaria and toxoplasmosis, an unusual cristae morphology has ...Mitochondrial ATP synthase plays a key role in inducing membrane curvature to establish cristae. In Apicomplexa causing diseases such as malaria and toxoplasmosis, an unusual cristae morphology has been observed, but its structural basis is unknown. Here, we report that the apicomplexan ATP synthase assembles into cyclic hexamers, essential to shape their distinct cristae. Cryo-EM was used to determine the structure of the hexamer, which is held together by interactions between parasite-specific subunits in the lumenal region. Overall, we identified 17 apicomplexan-specific subunits, and a minimal and nuclear-encoded subunit-a. The hexamer consists of three dimers with an extensive dimer interface that includes bound cardiolipins and the inhibitor IF. Cryo-ET and subtomogram averaging revealed that hexamers arrange into ~20-megadalton pentagonal pyramids in the curved apical membrane regions. Knockout of the linker protein ATPTG11 resulted in the loss of pentagonal pyramids with concomitant aberrantly shaped cristae. Together, this demonstrates that the unique macromolecular arrangement is critical for the maintenance of cristae morphology in Apicomplexa.
External links	Nat Commun / PubMed:33402698 / PubMed Central
Methods	EM (single particle) / EM (subtomogram averaging)
Resolution	2.8 - 34.0 Å
Structure data	10520 6tmg EMDB-10520: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, membrane region map PDB-6tmg: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, membrane region model Method: EM (single particle) / Resolution: 2.8 Å 10521 6tmh EMDB-10521: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, OSCP/F1/c-ring map PDB-6tmh: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, OSCP/F1/c-ring model Method: EM (single particle) / Resolution: 3.1 Å 10522 6tmi EMDB-10522: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, peripheral stalk map PDB-6tmi: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, peripheral stalk model Method: EM (single particle) / Resolution: 3.5 Å 10523 6tmj EMDB-10523: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, rotor-stator map PDB-6tmj: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, rotor-stator model Method: EM (single particle) / Resolution: 3.5 Å 10524 6tmk EMDB-10524: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, consensus map PDB-6tmk: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, composite model Method: EM (single particle) / Resolution: 2.9 Å 10525 6tml EMDB-10525: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase hexamer, membrane region PDB-6tml: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase hexamer, composite model Method: EM (single particle) / Resolution: 4.8 Å EMDB-10526: Subtomogram average of ATP synthase dimer from Toxoplasma gondii mitochondrial membranes Method: EM (subtomogram averaging) / Resolution: 20.0 Å EMDB-10527: Subtomogram average of the ATP synthase dimer from Toxoplasma gondii mitochondria Method: EM (subtomogram averaging) / Resolution: 34.0 Å EMDB-11403: Subtomogram average of the ATP synthase dimer from Toxoplasma gondii ATPTG11-KO mitochondrial membranes Method: EM (subtomogram averaging) / Resolution: 22.0 Å
Chemicals	ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipidYM / Phosphatidylcholine ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / detergentYM ChemComp-CDL: CARDIOLIPIN / phospholipidYM / Cardiolipin ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipidYM / Discrete optimized protein energy ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM / Adenosine triphosphate ChemComp-MG: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM / Adenosine diphosphate
Source	toxoplasma gondii gt1 (eukaryote) toxoplasma gondii (strain atcc 50853 / gt1) (eukaryote)
Keywords	MEMBRANE PROTEIN / mitochondrial / ATP synthase / membrane region / lipids / F1 / c-ring / peripheral stalk / OSCP / rotor / stator / dimer / hexamer