EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 120, Year 2021
掲載日	2021年1月5日
著者	Alexander Mühleip / Rasmus Kock Flygaard / Jana Ovciarikova / Alice Lacombe / Paula Fernandes / Lilach Sheiner / Alexey Amunts /
PubMed 要旨	Mitochondrial ATP synthase plays a key role in inducing membrane curvature to establish cristae. In Apicomplexa causing diseases such as malaria and toxoplasmosis, an unusual cristae morphology has ...Mitochondrial ATP synthase plays a key role in inducing membrane curvature to establish cristae. In Apicomplexa causing diseases such as malaria and toxoplasmosis, an unusual cristae morphology has been observed, but its structural basis is unknown. Here, we report that the apicomplexan ATP synthase assembles into cyclic hexamers, essential to shape their distinct cristae. Cryo-EM was used to determine the structure of the hexamer, which is held together by interactions between parasite-specific subunits in the lumenal region. Overall, we identified 17 apicomplexan-specific subunits, and a minimal and nuclear-encoded subunit-a. The hexamer consists of three dimers with an extensive dimer interface that includes bound cardiolipins and the inhibitor IF. Cryo-ET and subtomogram averaging revealed that hexamers arrange into ~20-megadalton pentagonal pyramids in the curved apical membrane regions. Knockout of the linker protein ATPTG11 resulted in the loss of pentagonal pyramids with concomitant aberrantly shaped cristae. Together, this demonstrates that the unique macromolecular arrangement is critical for the maintenance of cristae morphology in Apicomplexa.
リンク	Nat Commun / PubMed:33402698 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	2.8 - 34.0 Å
構造データ	10520 6tmg EMDB-10520: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, membrane region map PDB-6tmg: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, membrane region model 手法: EM (単粒子) / 解像度: 2.8 Å 10521 6tmh EMDB-10521: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, OSCP/F1/c-ring map PDB-6tmh: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, OSCP/F1/c-ring model 手法: EM (単粒子) / 解像度: 3.1 Å 10522 6tmi EMDB-10522: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, peripheral stalk map PDB-6tmi: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, peripheral stalk model 手法: EM (単粒子) / 解像度: 3.5 Å 10523 6tmj EMDB-10523: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, rotor-stator map PDB-6tmj: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, rotor-stator model 手法: EM (単粒子) / 解像度: 3.5 Å 10524 6tmk EMDB-10524: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, consensus map PDB-6tmk: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, composite model 手法: EM (単粒子) / 解像度: 2.9 Å 10525 6tml EMDB-10525: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase hexamer, membrane region PDB-6tml: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase hexamer, composite model 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-10526: Subtomogram average of ATP synthase dimer from Toxoplasma gondii mitochondrial membranes 手法: EM (サブトモグラム平均) / 解像度: 20.0 Å EMDB-10527: Subtomogram average of the ATP synthase dimer from Toxoplasma gondii mitochondria 手法: EM (サブトモグラム平均) / 解像度: 34.0 Å EMDB-11403: Subtomogram average of the ATP synthase dimer from Toxoplasma gondii ATPTG11-KO mitochondrial membranes 手法: EM (サブトモグラム平均) / 解像度: 22.0 Å
化合物	ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, リン脂質YM / Discrete optimized protein energy ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	toxoplasma gondii gt1 (トキソプラズマ) toxoplasma gondii (strain atcc 50853 / gt1) (トキソプラズマ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / mitochondrial (ミトコンドリア) / ATP synthase (ATP合成酵素) / membrane region / lipids (脂質) / F1 / c-ring (土星の環) / peripheral stalk / OSCP / rotor / stator (固定子) / dimer / hexamer (オリゴマー)