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Yorodumi- PDB-8cgt: STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED WITH A TH... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8cgt | |||||||||
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Title | STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED WITH A THIO-MALTOHEXAOSE | |||||||||
Components | PROTEIN (CYCLODEXTRIN-GLYCOSYLTRANSFERASE) | |||||||||
Keywords | TRANSFERASE / GLYCOSYLTRANSFERASE / STARCH DEGRADATION / CYCLODEXTRIN | |||||||||
Function / homology | Function and homology information cyclomaltodextrin glucanotransferase / cyclomaltodextrin glucanotransferase activity / starch binding / alpha-amylase activity / carbohydrate metabolic process / extracellular region / metal ion binding Similarity search - Function | |||||||||
Biological species | Bacillus circulans (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / OTHER / Resolution: 2.4 Å | |||||||||
Authors | Schmidt, A.K. / Schulz, G.E. | |||||||||
Citation | Journal: Eur.J.Biochem. / Year: 1998 Title: Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the gamma-cyclodextrin production. Authors: Parsiegla, G. / Schmidt, A.K. / Schulz, G.E. #1: Journal: Biochemistry / Year: 1998 Title: Structure of Cyclodextrin Glycosyltransferase Complexed with a Derivative of its Main Product Beta-Cyclodextrin Authors: Schmidt, A.K. / Cottaz, S. / Driguez, H. / Schulz, G.E. #2: Journal: Biochemistry / Year: 1992 Title: Catalytic Center of Cyclodextrin Glycosyltransferase Derived from X-Ray Structure Analysis Combined with Site-Directed Mutagenesis Authors: Klein, C. / Hollender, J. / Bender, H. / Schulz, G.E. #3: Journal: J.Mol.Biol. / Year: 1991 Title: Structure of Cyclodextrin Glycosyltransferase Refined at 2.0 A Resolution Authors: Klein, C. / Schulz, G.E. #4: Journal: Appl.Microbiol.Biotechnol. / Year: 1990 Title: Molecular Cloning, Nucleotide Sequence and Expression in Escherichia Coli of the Beta-Cyclodextrin Glycosyltransferase Gene from Bacillus Circulans Strain No. 8 Authors: Nitschke, L. / Heeger, K. / Bender, H. / Schulz, G.E. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8cgt.cif.gz | 147.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8cgt.ent.gz | 114.9 KB | Display | PDB format |
PDBx/mmJSON format | 8cgt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cg/8cgt ftp://data.pdbj.org/pub/pdb/validation_reports/cg/8cgt | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 74507.008 Da / Num. of mol.: 1 / Mutation: E257A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus circulans (bacteria) / Strain: 8 / Cellular location: EXTRACELLULARGlossary of biology / Production host: Escherichia coli (E. coli) / Strain (production host): TG1 References: UniProt: P30920, cyclomaltodextrin glucanotransferase | ||
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#2: Polysaccharide | alpha-D-glucopyranose-(1-4)-4-thio-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-4-thio- ...alpha-D-glucopyranose-(1-4)-4-thio-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-4-thio-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-4-thio-alpha-D-glucopyranose / thio-maltohexaose | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.78 Å3/Da / Density % sol: 67.43 % |
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Crystal grow | pH: 6.9 / Details: pH 6.9 |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Nov 1, 1996 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→34.317 Å / Num. obs: 48544 / % possible obs: 84.2 % / Redundancy: 3.2 % / Rsym value: 0.045 / Net I/σ(I): 15.1 |
Reflection shell | Resolution: 2.2→2.26 Å / Redundancy: 1.6 % / Mean I/σ(I) obs: 4.3 / Rsym value: 0.173 / % possible all: 51.9 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER / Resolution: 2.4→30 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.4→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.51 Å / Total num. of bins used: 8
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Xplor file |
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