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- PDB-6xdh: Crystal Structure of NendoU (Uridylate-specific endoribonuclease,... -

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Basic information

Entry
Database: PDB / ID: 6xdh
TitleCrystal Structure of NendoU (Uridylate-specific endoribonuclease, nsp15) from Betacoronavirus SARS-CoV-2
ComponentsUridylate-specific endoribonuclease
KeywordsHydrolase/VIRAL PROTEIN / SSGCID / endoribonuclease / nsp15 / COVID-19 / SARS-CoV-2 / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease / VIRAL PROTEIN / Hydrolase-VIRAL PROTEIN complex
Function / homology
Function and homology information


protein guanylyltransferase activity / RNA endonuclease activity, producing 3'-phosphomonoesters / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / Maturation of replicase proteins / ISG15-specific peptidase activity / Transcription of SARS-CoV-2 sgRNAs ...protein guanylyltransferase activity / RNA endonuclease activity, producing 3'-phosphomonoesters / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / Maturation of replicase proteins / ISG15-specific peptidase activity / Transcription of SARS-CoV-2 sgRNAs / Translation of Replicase and Assembly of the Replication Transcription Complex / TRAF3-dependent IRF activation pathway / snRNP Assembly / Replication of the SARS-CoV-2 genome / double membrane vesicle viral factory outer membrane / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / SARS coronavirus main proteinase / host cell endosome / 3'-5'-RNA exonuclease activity / : / host cell endoplasmic reticulum-Golgi intermediate compartment / symbiont-mediated suppression of host toll-like receptor signaling pathway / symbiont-mediated degradation of host mRNA / mRNA guanylyltransferase / symbiont-mediated suppression of host ISG15-protein conjugation / G-quadruplex RNA binding / SARS-CoV-2 modulates host translation machinery / omega peptidase activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / host cell Golgi apparatus / symbiont-mediated perturbation of host ubiquitin-like protein modification / mRNA (nucleoside-2'-O-)-methyltransferase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / DNA helicase / ubiquitinyl hydrolase 1 / cysteine-type deubiquitinase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / single-stranded RNA binding / host cell endoplasmic reticulum membrane / host cell perinuclear region of cytoplasm / viral protein processing / lyase activity / RNA helicase / induction by virus of host autophagy / RNA-directed RNA polymerase / copper ion binding / symbiont-mediated suppression of host gene expression / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / DNA-templated transcription / lipid binding / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / host cell nucleus / SARS-CoV-2 activates/modulates innate and adaptive immune responses / ATP hydrolysis activity / proteolysis / RNA binding / zinc ion binding / ATP binding / membrane
Similarity search - Function
Nsp15 N-terminal domain-like / nsp15 middle domain / Double Stranded RNA Binding Domain / RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / : / : / Coronavirus Nonstructural protein 13, 1B domain / Coronavirus Non-structural protein 13, zinc-binding domain ...Nsp15 N-terminal domain-like / nsp15 middle domain / Double Stranded RNA Binding Domain / RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / : / : / Coronavirus Nonstructural protein 13, 1B domain / Coronavirus Non-structural protein 13, zinc-binding domain / Coronavirus Nonstructural protein 13, stalk domain / : / Coronavirus Nsp12 Interface domain profile. / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP14, guanine-N7-methyltransferase domain, coronavirus / NSP12 RNA-dependent RNA polymerase, coronavirus / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / Nidovirus 2-O-methyltransferase / Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain profile. / Nidovirus 3'-5' exoribonuclease domain / Nidovirus 2'-O-methyltransferase (2'-O-MTase) domain profile. / Nidovirus 3'-5' exoribonuclease (ExoN) domain profile. / Nonstructural protein 13, 1B domain, coronavirus / Arterivirus Nsp11 N-terminal/Coronavirus NSP15 middle domain / Non-structural protein NSP15, N-terminal domain superfamily, coronavirus / Non-structural protein NSP15, middle domain superfamily / Coronavirus replicase NSP15, N-terminal oligomerization / Nonstructural protein 15, middle domain, coronavirus / Coronavirus replicase NSP15, middle domain / Coronavirus replicase NSP15, N-terminal oligomerisation / Arterivirus Nsp11 N-terminal/coronavirus NSP15 middle (AV-Nsp11N/CoV-Nsp15M) domain profile. / Non-structural protein NSP16, coronavirus-like / Non-structural protein 14, coronavirus / RNA polymerase, N-terminal, coronavirus / Coronavirus 2'-O-methyltransferase / Coronavirus proofreading exoribonuclease / Coronavirus RNA-dependent RNA polymerase, N-terminal / Nidoviral uridylate-specific endoribonuclease (NendoU) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain / Nonstructural protein 13, zinc-binding domain, coronavirus-like / Coronaviridae zinc-binding (CV ZBD) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain profile. / Endoribonuclease EndoU-like / NendoU domain, nidovirus / Coronavirus replicase NSP15, uridylate-specific endoribonuclease / DNA2/NAM7 helicase-like, C-terminal / AAA domain / (+) RNA virus helicase core domain / (+)RNA virus helicase core domain profile. / Lipocalin signature. / Non-structural protein NSP3, SUD-N (Mac2) domain, betacoronavirus / Sarbecovirus Nsp3c-N domain profile. / Non-structural protein NSP3, N-terminal, betacoronavirus / Polyprotein cleavage domain PL2pro superfamily, betacoronavirus / Non-structural protein NSP3, SUD-N (Mac2) domain superfamily, betacoronavirus / Betacoronavirus SUD-C domain / Betacoronavirus replicase NSP3, N-terminal / NSP1 globular domain superfamily, betacoronavirus / Non-structural protein 2, SARS-CoV-like / Carbamoyl-phosphate synthase subdomain signature 2. / Coronavirus 3Ecto domain profile. / : / Betacoronavirus Nsp3e group 2-specific marker (G2M) domain profile. / NSP1, C-terminal domain, betacoronavirus / Betacoronavirus Nsp3c-M domain profile. / NSP1, globular domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain superfamily, betacoronavirus / Betacoronavirus replicase NSP1 / Betacoronavirus single-stranded poly(A) binding domain / Betacoronavirus (BetaCoV) Nsp1 C-terminal domain profile. / Betacoronavirus Nsp3c-C domain profile. / Betacoronavirus Nsp3e nucleic acid-binding (NAB) domain profile. / DPUP/SUD, C-terminal, betacoronavirus / Non-structural protein NSP3, nucleic acid-binding domain superfamily, betacoronavirus / Non-structural protein 6, betacoronavirus / Betacoronavirus nucleic acid-binding (NAB) / Non-structural protein NSP3, nucleic acid-binding domain, betacoronavirus / Non-structural protein NSP3A domain-like superfamily / Papain-like protease, N-terminal domain superfamily, coronavirus / Papain-like viral protease, palm and finger domains, coronavirus / : / Coronavirus (CoV) Nsp2 middle domain profile. / Coronavirus (CoV) Nsp2 N-terminal domain profile. / Coronavirus (CoV) Nsp2 C-terminal domain profile. / NSP1, globular domain, alpha/betacoronavirus / : / Coronavirus (CoV) Nsp3 Y domain profile. / Coronavirus (CoV) Nsp1 globular domain profile. / Coronavirus replicase NSP2, N-terminal / Nonstructural protein 2, N-terminal domain, coronavirus / Coronavirus replicase NSP2, C-terminal / Non-structural protein 2, C-terminal domain, coronavirus / Coronavirus Nsp3a Ubl domain profile. / Coronavirus Nsp3d Ubl domain profile. / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp7 cofactor domain profile. / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp8 cofactor domain profile. / Coronavirus Nsp9 single-stranded RNA (ssRNA)-binding domain profile.
Similarity search - Domain/homology
ACETATE ION / CITRIC ACID / FORMIC ACID / Replicase polyprotein 1ab
Similarity search - Component
Biological speciesSevere acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.35 Å
AuthorsSeattle Structural Genomics Center for Infectious Disease / Seattle Structural Genomics Center for Infectious Disease (SSGCID)
CitationJournal: to be published
Title: Crystal Structure of NendoU (Uridylate-specific endoribonuclease, nsp15) from Betacoronavirus SARS-CoV-2
Authors: Dranow, D.M. / Abendroth, J. / Lorimer, D.D. / Horanyi, P.S. / Edwards, T.E.
History
DepositionJun 10, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 17, 2020Provider: repository / Type: Initial release
Revision 1.1Sep 16, 2020Group: Data collection / Category: diffrn_detector / Item: _diffrn_detector.pdbx_collection_date
Revision 1.2Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Uridylate-specific endoribonuclease
B: Uridylate-specific endoribonuclease
hetero molecules


Theoretical massNumber of molelcules
Total (without water)83,64713
Polymers82,7942
Non-polymers85411
Water7,584421
1
A: Uridylate-specific endoribonuclease
hetero molecules

A: Uridylate-specific endoribonuclease
hetero molecules

A: Uridylate-specific endoribonuclease
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,22015
Polymers124,1913
Non-polymers1,03012
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_665-y+1,x-y+1,z1
crystal symmetry operation3_565-x+y,-x+1,z1
Buried area9790 Å2
ΔGint-47 kcal/mol
Surface area43430 Å2
MethodPISA
2
B: Uridylate-specific endoribonuclease
hetero molecules

B: Uridylate-specific endoribonuclease
hetero molecules

B: Uridylate-specific endoribonuclease
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,72224
Polymers124,1913
Non-polymers1,53121
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_565-y,x-y+1,z1
crystal symmetry operation3_455-x+y-1,-x,z1
Buried area10700 Å2
ΔGint-36 kcal/mol
Surface area43880 Å2
MethodPISA
Unit cell
Length a, b, c (Å)150.820, 150.820, 112.570
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number173
Space group name H-MP63
Space group name HallP6c
Symmetry operation#1: x,y,z
#2: x-y,x,z+1/2
#3: y,-x+y,z+1/2
#4: -y,x-y,z
#5: -x+y,-x,z
#6: -x,-y,z+1/2

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Uridylate-specific endoribonuclease / NendoU / nsp15


Mass: 41396.930 Da / Num. of mol.: 2 / Fragment: BewuA.18928.a.MX151
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Plasmid: BewuA.18928.a.MX151 / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: P0DTD1, Hydrolases; Acting on ester bonds

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Non-polymers , 5 types, 432 molecules

#2: Chemical ChemComp-ACT / ACETATE ION / Acetate


Mass: 59.044 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C2H3O2
#3: Chemical ChemComp-CIT / CITRIC ACID / Citric acid


Mass: 192.124 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H8O7
#4: Chemical
ChemComp-FMT / FORMIC ACID / Formic acid


Mass: 46.025 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: CH2O2
#5: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C2H6O2
#6: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 421 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.46 Å3/Da / Density % sol: 72.45 %
Crystal growTemperature: 287 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: BewuA.18928.a.MX151.PW38806 at 36 mg/ml mixed 1:1 with Morpheus(G3): 10% (w/v) PEG 4000, 20% (v/v) glycerol, 0.1 M MES/imidazole, pH=6.5, 0.02 M of each sodium formate, ammonium acetate, ...Details: BewuA.18928.a.MX151.PW38806 at 36 mg/ml mixed 1:1 with Morpheus(G3): 10% (w/v) PEG 4000, 20% (v/v) glycerol, 0.1 M MES/imidazole, pH=6.5, 0.02 M of each sodium formate, ammonium acetate, trisodium citrate, sodium potassium l-tartrate, sodium oxamate. Tray: 315968g8, puck: teq6-3.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.9787 Å
DetectorType: RAYONIX MX-300 / Detector: CCD / Date: Apr 24, 2020 / Details: Beryllium Lenses
RadiationMonochromator: Diamond [111] / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9787 Å / Relative weight: 1
ReflectionResolution: 2.35→50 Å / Num. obs: 60593 / % possible obs: 100 % / Redundancy: 11.456 % / Biso Wilson estimate: 46.957 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.077 / Rrim(I) all: 0.081 / Χ2: 0.923 / Net I/σ(I): 22.57
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsCC1/2Rrim(I) all% possible all
2.35-2.4111.4860.5944.1544540.950.621100
2.41-2.4811.50.4825.0443680.9680.504100
2.48-2.5511.5170.4115.9341960.9760.43100
2.55-2.6311.5110.3516.9441120.9790.367100
2.63-2.7111.5220.2798.6339970.9870.292100
2.71-2.8111.5240.20911.4338720.9920.219100
2.81-2.9111.530.17113.936990.9940.179100
2.91-3.0311.5370.14116.5335990.9960.147100
3.03-3.1711.5160.1120.6934190.9980.115100
3.17-3.3211.5090.08825.1832910.9980.092100
3.32-3.511.4710.0731.2231500.9980.074100
3.5-3.7211.4470.06135.729540.9990.064100
3.72-3.9711.4260.05539.127950.9990.058100
3.97-4.2911.4040.04844.8225960.9990.05100
4.29-4.711.410.04447.9323980.9990.046100
4.7-5.2511.4020.04249.2821660.9990.043100
5.25-6.0711.4130.04347.1919260.9990.045100
6.07-7.4311.3210.04148.6616210.9990.043100
7.43-10.5111.0950.03855.4812730.9990.039100
10.51-509.9280.03954.997070.9990.04198.3

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
PHENIX1.18.2_3874refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
PDB_EXTRACT3.25data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6vww

6vww


Resolution: 2.35→45.21 Å / SU ML: 0.1908 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 20.2715
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.1825 2078 3.43 %
Rwork0.1572 58473 -
obs0.1581 60551 99.97 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 52.51 Å2
Refinement stepCycle: LAST / Resolution: 2.35→45.21 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5450 0 57 421 5928
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00665663
X-RAY DIFFRACTIONf_angle_d0.80127694
X-RAY DIFFRACTIONf_chiral_restr0.0547883
X-RAY DIFFRACTIONf_plane_restr0.0047995
X-RAY DIFFRACTIONf_dihedral_angle_d19.42442055
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.35-2.40.25121130.19413879X-RAY DIFFRACTION99.97
2.4-2.460.21181390.19093899X-RAY DIFFRACTION100
2.46-2.530.23881390.18653859X-RAY DIFFRACTION99.93
2.53-2.610.20911320.19033925X-RAY DIFFRACTION99.98
2.61-2.690.22371450.19013866X-RAY DIFFRACTION100
2.69-2.790.2041400.18043855X-RAY DIFFRACTION99.97
2.79-2.90.23791470.18053920X-RAY DIFFRACTION100
2.9-3.030.23691440.18333870X-RAY DIFFRACTION99.98
3.03-3.190.21551150.17623925X-RAY DIFFRACTION99.98
3.19-3.390.19171300.16813892X-RAY DIFFRACTION100
3.39-3.650.17751510.15613874X-RAY DIFFRACTION100
3.65-4.020.16661300.1443934X-RAY DIFFRACTION100
4.02-4.60.14581460.12813903X-RAY DIFFRACTION100
4.6-5.790.13571430.13363909X-RAY DIFFRACTION99.98
5.79-45.210.1741640.14673963X-RAY DIFFRACTION99.73
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.58855622364-0.03488861808031.915421873717.503654419191.369118839985.438565055350.2339938929950.302865117678-0.6048768504430.2047278495770.0295479741779-0.4518937088380.6586120855360.141166320865-0.2164811499410.3360905763480.0330690256118-0.02416853672940.302801742058-0.03890528560120.265038821213-3.095661173466.321489411148.287738331
23.1371259890.2485486433111.113030910681.8695348694-0.1651764411361.92224263364-0.02428522720940.213986199831-0.131042586534-0.1757729955080.07401451026030.3240974681420.160810769895-0.147831121366-0.03940925158350.269217449282-0.0453343279236-0.0329053577120.269826320178-0.04237028991810.276959849384-23.68357129673.547113507340.3072088963
31.686159292130.413977392397-0.2339621095073.28273172004-0.5898085318611.20761785076-0.1623059776890.7547875286530.16116728431-1.023597255340.2270308324480.32112685648-0.121473349248-0.161723375461-0.05117372123830.713642173101-0.104338582674-0.151256096260.6431946514030.0810989570580.361105863847-21.215750682395.566317575821.2252942653
45.00724670496-1.46151510048-0.6461539101477.76929355035-2.788519670784.14799960790.3586777198060.4333459884220.952435713904-0.2187093817790.1559501595841.47937581165-0.740296183454-0.337978817191-0.4121985565890.6152684656150.00637016034942-0.1878187193830.5856665407430.1554533736750.828167223616-33.7681669419101.68899449825.4936079599
55.766113523530.4795693583911.393926985528.282467185140.04645311882885.404789047630.1142977008660.0311792239784-0.4659403410750.04532410914170.0112425551352-0.06494429203110.4724412994970.274169051506-0.1522320887050.3220196226380.00533486620068-0.027493746760.233944789867-0.02328320575990.199145659386-62.551827017626.86075088137.8986912597
61.860295678750.671722473180.7939527031143.082312402991.471366282992.755678932440.1086343371670.0822690931799-0.0875547967156-0.0271959514090.270628385766-0.8977263023240.05879652456320.538736356132-0.2348789285320.224077624610.00614182445888-0.008687025709280.323218602504-0.06261024628560.512789754436-48.587186701442.643281918215.7771504856
73.711464453130.0963061724658-1.172651429543.092029012991.176532917663.58293309532-0.0459978209106-0.437042635237-0.2040651621450.4720779311810.126425204637-0.4919286312160.106152680610.178368048182-0.1673789517890.349679116874-0.0434824445834-0.1486180770060.387740932758-0.002791779387480.441164345047-52.019894427655.978202981935.56205825
83.14910161241-0.778463964116-0.4537154117022.480233207150.58408045441.71267914130.123558391579-0.3541995997920.3860762686760.306941048349-0.110843285604-0.113725837076-0.0924098122672-0.0591262648919-0.02920892419810.292230332157-0.0675984676359-0.01539500381230.36302871915-0.08785988883790.32566350246-62.075699619964.544854305232.8787908794
95.03202910811-4.15222547751-2.733710072824.184308541551.237208988673.112621394390.5824278520930.1975201522131.03944246996-0.11320952136-0.109125277002-0.371011472605-0.300667262944-0.633240779622-0.3936411917620.389975651237-0.0750464678954-0.008001794396350.503489146172-0.08680698851680.590080898473-56.094859156273.050499547530.1561293782
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 0 through 38 )AA0 - 381 - 43
22chain 'A' and (resid 39 through 183 )AA39 - 18344 - 190
33chain 'A' and (resid 184 through 309 )AA184 - 309191 - 316
44chain 'A' and (resid 310 through 346 )AA310 - 346317 - 353
55chain 'B' and (resid -1 through 38 )BF-1 - 381 - 42
66chain 'B' and (resid 39 through 177 )BF39 - 17743 - 187
77chain 'B' and (resid 178 through 225 )BF178 - 225188 - 235
88chain 'B' and (resid 226 through 325 )BF226 - 325236 - 337
99chain 'B' and (resid 326 through 347 )BF326 - 347338 - 359

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