+Open data
-Basic information
Entry | Database: PDB / ID: 6ib8 | ||||||
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Title | Structure of a complex of SuhB and NusA AR2 domain | ||||||
Components |
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Keywords | TRANSCRIPTION / SuhB / NusA AR2 domain / antitermiantion | ||||||
Function / homology | Function and homology information glycerol-2-phosphatase activity / lithium ion binding / inositol-phosphate phosphatase / inositol monophosphate 3-phosphatase activity / inositol monophosphate 4-phosphatase activity / inositol monophosphate 1-phosphatase activity / inositol metabolic process / bacterial-type RNA polymerase core enzyme binding / rRNA primary transcript binding / transcription antitermination factor activity, RNA binding ...glycerol-2-phosphatase activity / lithium ion binding / inositol-phosphate phosphatase / inositol monophosphate 3-phosphatase activity / inositol monophosphate 4-phosphatase activity / inositol monophosphate 1-phosphatase activity / inositol metabolic process / bacterial-type RNA polymerase core enzyme binding / rRNA primary transcript binding / transcription antitermination factor activity, RNA binding / phosphatidylinositol phosphate biosynthetic process / transcription elongation factor complex / regulation of DNA-templated transcription elongation / transcription antitermination / DNA-templated transcription termination / ribosome biogenesis / protein complex oligomerization / DNA-binding transcription factor activity / protein domain specific binding / nucleotide binding / magnesium ion binding / signal transduction / RNA binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.646 Å | ||||||
Authors | Huang, Y.H. / Loll, B. / Wahl, M.C. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2019 Title: Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis. Authors: Huang, Y.H. / Said, N. / Loll, B. / Wahl, M.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6ib8.cif.gz | 140.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6ib8.ent.gz | 107.9 KB | Display | PDB format |
PDBx/mmJSON format | 6ib8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ib/6ib8 ftp://data.pdbj.org/pub/pdb/validation_reports/ib/6ib8 | HTTPS FTP |
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-Related structure data
Related structure data | 6ib7C 1wcnS 2qflS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 2 types, 3 molecules ABC
#1: Protein | Mass: 29537.502 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: suhB, ssyA, b2533, JW2517 / Plasmid: pETM-11 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0ADG4, inositol-phosphate phosphatase #2: Protein | | Mass: 7671.543 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: nusA, ECs4050 / Plasmid: pETM-11 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: C3SSP1, UniProt: P0AFF6*PLUS |
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-Non-polymers , 4 types, 275 molecules
#3: Chemical | ChemComp-GOL / #4: Chemical | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.84 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.1M Tris-HCl pH 8.5, 5% (w/v) PEG 8000, 16% (v/v) PEG 300, 10% (v/v) glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: BESSY / Beamline: 14.2 / Wavelength: 0.91841 Å |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Oct 6, 2017 |
Radiation | Monochromator: KMC-2 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91841 Å / Relative weight: 1 |
Reflection | Resolution: 1.646→50 Å / Num. obs: 78162 / % possible obs: 99.1 % / Redundancy: 5.5 % / CC1/2: 0.99 / Net I/σ(I): 15.2 |
Reflection shell | Resolution: 1.646→1.74 Å / Redundancy: 5.1 % / Mean I/σ(I) obs: 0.92 / Num. unique obs: 12069 / CC1/2: 0.36 / Rrim(I) all: 1.86 / % possible all: 95.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2QFL, 1WCN Resolution: 1.646→30.634 Å / SU ML: 0.25 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 22.84
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.646→30.634 Å
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Refine LS restraints |
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LS refinement shell |
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