+Open data
-Basic information
Entry | Database: PDB / ID: 6f0k | |||||||||
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Title | Alternative complex III | |||||||||
Components |
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Keywords | MEMBRANE PROTEIN / electron transfer / quinol oxidation / respiratory chain | |||||||||
Function / homology | Function and homology information electron transfer activity / membrane => GO:0016020 / heme binding / metal ion binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Rhodothermus marinus (bacteria) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.87 Å | |||||||||
Authors | Sousa, J.S. / Calisto, F. / Mills, D.J. / Pereira, M.M. / Vonck, J. / Kuehlbrandt, W. | |||||||||
Funding support | Portugal, Germany, 2items
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Citation | Journal: Nat Commun / Year: 2018 Title: Structural basis for energy transduction by respiratory alternative complex III. Authors: Joana S Sousa / Filipa Calisto / Julian D Langer / Deryck J Mills / Patrícia N Refojo / Miguel Teixeira / Werner Kühlbrandt / Janet Vonck / Manuela M Pereira / Abstract: Electron transfer in respiratory chains generates the electrochemical potential that serves as energy source for the cell. Prokaryotes can use a wide range of electron donors and acceptors and may ...Electron transfer in respiratory chains generates the electrochemical potential that serves as energy source for the cell. Prokaryotes can use a wide range of electron donors and acceptors and may have alternative complexes performing the same catalytic reactions as the mitochondrial complexes. This is the case for the alternative complex III (ACIII), a quinol:cytochrome c/HiPIP oxidoreductase. In order to understand the catalytic mechanism of this respiratory enzyme, we determined the structure of ACIII from Rhodothermus marinus at 3.9 Å resolution by single-particle cryo-electron microscopy. ACIII presents a so-far unique structure, for which we establish the arrangement of the cofactors (four iron-sulfur clusters and six c-type hemes) and propose the location of the quinol-binding site and the presence of two putative proton pathways in the membrane. Altogether, this structure provides insights into a mechanism for energy transduction and introduces ACIII as a redox-driven proton pump. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6f0k.cif.gz | 830.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6f0k.ent.gz | 706.9 KB | Display | PDB format |
PDBx/mmJSON format | 6f0k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f0/6f0k ftp://data.pdbj.org/pub/pdb/validation_reports/f0/6f0k | HTTPS FTP |
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-Related structure data
Related structure data | 4165MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Protein , 7 types, 7 molecules ABCDEFH
#1: Protein | Mass: 24245.594 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (bacteria) Strain: ATCC 43812 / DSM 4252 / R-10 / References: UniProt: D0MDD4 |
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#2: Protein | Mass: 115382.617 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (bacteria) Strain: ATCC 43812 / DSM 4252 / R-10 / References: UniProt: D0MDD5 |
#3: Protein | Mass: 55256.566 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (bacteria) Strain: ATCC 43812 / DSM 4252 / R-10 / References: UniProt: D0MDD6 |
#4: Protein | Mass: 23796.275 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (bacteria) Strain: ATCC 43812 / DSM 4252 / R-10 / References: UniProt: D0MDD7 |
#5: Protein | Mass: 23336.631 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (bacteria) Strain: ATCC 43812 / DSM 4252 / R-10 / References: UniProt: D0MDD8 |
#6: Protein | Mass: 48536.781 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (bacteria) Strain: ATCC 43812 / DSM 4252 / R-10 / References: UniProt: D0MDD9 |
#7: Protein | Mass: 20219.014 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (bacteria) Strain: ATCC 43812 / DSM 4252 / R-10 / References: UniProt: D0MKF0 |
-Non-polymers , 3 types, 10 molecules
#8: Chemical | ChemComp-HEC / #9: Chemical | ChemComp-F3S / | #10: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Alternative complex III / Type: COMPLEX / Entity ID: #1-#7 / Source: NATURAL |
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Molecular weight | Value: 300 kDa/nm / Experimental value: NO |
Source (natural) | Organism: Rhodothermus marinus (bacteria) / Strain: PRQ-62B |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy |
Image recording | Electron dose: 72 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.12_2829: / Classification: refinement | ||||||||||||||||||||||||
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EM software | Name: RELION / Category: 3D reconstruction | ||||||||||||||||||||||||
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
Symmetry | Point symmetry: C1 (asymmetric) | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.87 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 52386 / Symmetry type: POINT | ||||||||||||||||||||||||
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