+Open data
-Basic information
Entry | Database: PDB / ID: 6d53 | ||||||
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Title | Trans form of HemolysinII c-terminal domain | ||||||
Components | Hemolysin II | ||||||
Keywords | TOXIN / pore-forming toxin / proline isomerization / conformational heterogeneity | ||||||
Function / homology | Bi-component toxin, staphylococci / Leukocidin/Hemolysin toxin / Leukocidin/Hemolysin toxin family / Leukocidin/porin MspA superfamily / cytolysis in another organism / extracellular region / Hemolysin II Function and homology information | ||||||
Biological species | Bacillus cereus (bacteria) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Kaplan, A.R. / Alexandrescu, A.T. / Olson, R. | ||||||
Citation | Journal: To Be Published Title: Trans form of HemolysinII c-terminal domain Authors: Kaplan, A.R. / Alexandrescu, A.T. / Olson, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6d53.cif.gz | 700.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6d53.ent.gz | 589.9 KB | Display | PDB format |
PDBx/mmJSON format | 6d53.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/d5/6d53 ftp://data.pdbj.org/pub/pdb/validation_reports/d5/6d53 | HTTPS FTP |
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-Related structure data
Related structure data | 6d5zC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10841.098 Da / Num. of mol.: 1 / Fragment: C-terminal Domain, residues 319-412 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus cereus (bacteria) Strain: ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 Gene: BC_3523 / Plasmid: pET28b Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) Strain (production host): BL21-Gold(DE3)pLysS AG / References: UniProt: Q81AN8 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0 M / Label: 1 / pH: 6.0 / Pressure: 1 atm / Temperature: 310 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 7 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 25 |