+Open data
-Basic information
Entry | Database: PDB / ID: 5zx1 | ||||||
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Title | Crystal structure of ENT domain from T. brucei | ||||||
Components | ENT | ||||||
Keywords | TRANSCRIPTION / ENT domain | ||||||
Function / homology | ENT domain-like superfamily / nucleus / Uncharacterized protein Function and homology information | ||||||
Biological species | Trypanosoma brucei brucei (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.31 Å | ||||||
Authors | Shanhui, L. / Xiaoming, T. / Juan, M. | ||||||
Funding support | China, 1items
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Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2018 Title: Crystal structure of an ENT domain from Trypanosoma brucei. Authors: Mi, J. / Yang, X. / Zhang, J. / Zhang, X. / Xu, C. / Liao, S. / Tu, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5zx1.cif.gz | 31.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5zx1.ent.gz | 19.1 KB | Display | PDB format |
PDBx/mmJSON format | 5zx1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5zx1_validation.pdf.gz | 400.4 KB | Display | wwPDB validaton report |
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Full document | 5zx1_full_validation.pdf.gz | 401.7 KB | Display | |
Data in XML | 5zx1_validation.xml.gz | 3.7 KB | Display | |
Data in CIF | 5zx1_validation.cif.gz | 4.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zx/5zx1 ftp://data.pdbj.org/pub/pdb/validation_reports/zx/5zx1 | HTTPS FTP |
-Related structure data
Related structure data | 1uz3S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 13068.890 Da / Num. of mol.: 1 / Fragment: ENT domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.02.2890 / Production host: Escherichia coli (E. coli) / References: UniProt: Q385P5 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.37 Å3/Da / Density % sol: 63.45 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, sitting drop / Details: 0.2M L-proline, 0.1M HEPES pH7.5, 10% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.9791 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: May 12, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 2.31→46.88 Å / Num. obs: 8072 / % possible obs: 99 % / Redundancy: 12.5 % / Rmerge(I) obs: 0.028 / Rpim(I) all: 0.028 / Net I/σ(I): 14.5 |
Reflection shell | Resolution: 2.31→2.39 Å / Rmerge(I) obs: 0.27 / Num. unique obs: 1586 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1UZ3 Resolution: 2.31→46.878 Å / Cross valid method: NONE
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Refinement step | Cycle: LAST / Resolution: 2.31→46.878 Å
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LS refinement shell | Resolution: 2.31→2.39 Å
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