+Open data
-Basic information
Entry | Database: PDB / ID: 5gs5 | ||||||
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Title | Crystal structure of apo rat STING | ||||||
Components | Stimulator of interferon genes protein | ||||||
Keywords | IMMUNE SYSTEM / rat STING | ||||||
Function / homology | Function and homology information STING mediated induction of host immune responses / STAT6-mediated induction of chemokines / IRF3-mediated induction of type I IFN / Regulation of innate immune responses to cytosolic DNA / STING complex / 2',3'-cyclic GMP-AMP binding / cyclic-di-GMP binding / positive regulation of type I interferon-mediated signaling pathway / pattern recognition receptor signaling pathway / reticulophagy ...STING mediated induction of host immune responses / STAT6-mediated induction of chemokines / IRF3-mediated induction of type I IFN / Regulation of innate immune responses to cytosolic DNA / STING complex / 2',3'-cyclic GMP-AMP binding / cyclic-di-GMP binding / positive regulation of type I interferon-mediated signaling pathway / pattern recognition receptor signaling pathway / reticulophagy / Neutrophil degranulation / cytoplasmic pattern recognition receptor signaling pathway / cellular response to exogenous dsRNA / autophagosome membrane / positive regulation of macroautophagy / cellular response to organic cyclic compound / autophagosome assembly / autophagosome / positive regulation of type I interferon production / cellular response to interferon-beta / signaling adaptor activity / antiviral innate immune response / positive regulation of defense response to virus by host / activation of innate immune response / positive regulation of interferon-beta production / endoplasmic reticulum-Golgi intermediate compartment membrane / protein serine/threonine kinase activator activity / molecular function activator activity / positive regulation of DNA-binding transcription factor activity / regulation of inflammatory response / positive regulation of protein binding / peroxisome / protein complex oligomerization / regulation of gene expression / defense response to virus / RNA polymerase II-specific DNA-binding transcription factor binding / mitochondrial outer membrane / endosome / Golgi membrane / innate immune response / ubiquitin protein ligase binding / endoplasmic reticulum membrane / protein kinase binding / perinuclear region of cytoplasm / Golgi apparatus / endoplasmic reticulum / positive regulation of transcription by RNA polymerase II / protein homodimerization activity / nucleoplasm / identical protein binding / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.84 Å | ||||||
Authors | Zhang, H. / Han, M.J. / Tao, J.L. / Ye, Z.Y. / Du, X.X. / Deng, M.J. / Zhang, X.Y. / Li, L.F. / Jiang, Z.F. / Su, X.D. | ||||||
Citation | Journal: To Be Published Title: Crystal structure of apo ratSTING Authors: Zhang, H. / Han, M.J. / Tao, J.L. / Ye, Z.Y. / Du, X.X. / Deng, M.J. / Zhang, X.Y. / Li, L.F. / Jiang, Z.F. / Su, X.D. #1: Journal: Sci Rep. / Year: 2015 Title: Rat and human STINGs profile similarly towards anticancer/antiviral compounds Authors: Zhang, H. / Han, M.J. / Tao, J.L. / Ye, Z.Y. / Du, X.X. / Deng, M.J. / Zhang, X.Y. / Li, L.F. / Jiang, Z.F. / Su, X.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5gs5.cif.gz | 342.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5gs5.ent.gz | 277.8 KB | Display | PDB format |
PDBx/mmJSON format | 5gs5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5gs5_validation.pdf.gz | 485.3 KB | Display | wwPDB validaton report |
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Full document | 5gs5_full_validation.pdf.gz | 502.8 KB | Display | |
Data in XML | 5gs5_validation.xml.gz | 39.6 KB | Display | |
Data in CIF | 5gs5_validation.cif.gz | 57.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gs/5gs5 ftp://data.pdbj.org/pub/pdb/validation_reports/gs/5gs5 | HTTPS FTP |
-Related structure data
Related structure data | 4lojS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Ens-ID: 1
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-Components
#1: Protein | Mass: 25324.426 Da / Num. of mol.: 4 / Fragment: UNP residues 140-337 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Tmem173, Sting / Production host: Escherichia coli (E. coli) / References: UniProt: F1M391 #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.4 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / Details: 200mM NaCl, 20mM MES pH6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-1A / Wavelength: 1.1 Å |
Detector | Type: DECTRIS PILATUS 2M / Detector: CCD / Date: Feb 17, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 1.84→50 Å / Num. obs: 70041 / % possible obs: 98.8 % / Redundancy: 3.75 % / Net I/σ(I): 12.54 |
Reflection shell | Resolution: 1.84→1.92 Å / Redundancy: 3.85 % / Rmerge(I) obs: 0.76 / Mean I/σ(I) obs: 2.23 / % possible all: 98.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4LOJ Resolution: 1.84→42.728 Å / SU ML: 0.23 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 22.06
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 102.25 Å2 / Biso mean: 25.37 Å2 / Biso min: 3.72 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.84→42.728 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 25
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Refinement TLS params. | Method: refined / Origin x: -10.9154 Å / Origin y: 67.7124 Å / Origin z: 22.9398 Å
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Refinement TLS group | Selection details: all |