+Open data
-Basic information
Entry | Database: PDB / ID: 4tkd | ||||||
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Title | Sulfolobus solfataricus HJC mutants | ||||||
Components | Holliday junction resolvase Hjc | ||||||
Keywords | HYDROLASE | ||||||
Function / homology | Function and homology information crossover junction endodeoxyribonuclease / crossover junction DNA endonuclease activity / DNA recombination / DNA repair / magnesium ion binding / DNA binding Similarity search - Function | ||||||
Biological species | Sulfolobus solfataricus (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.01 Å | ||||||
Authors | Bond, C.S. | ||||||
Citation | Journal: Aust.J.Chem. / Year: 2014 Title: Crystal Unengineering: Reducing the Crystallisability of Sulfolobus solfataricus Hjc Authors: Middleton, C.L. / Parker, J.L. / Knott, G.J. / White, M.F. / Bond, C.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4tkd.cif.gz | 104.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4tkd.ent.gz | 82 KB | Display | PDB format |
PDBx/mmJSON format | 4tkd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tk/4tkd ftp://data.pdbj.org/pub/pdb/validation_reports/tk/4tkd | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15797.519 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (archaea) / Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2 / Gene: hjc, SSO0575, ORF-c21_024 / Production host: Escherichia coli (E. coli) References: UniProt: Q7LXU0, crossover junction endodeoxyribonuclease #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop Details: 10 % (w/v) PEG 6000, 5 % (v/v) PEG 400, and 0.1 M citric acid pH 5.0 |
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-Data collection
Diffraction | Mean temperature: 103.15 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-2 / Wavelength: 0.933 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: May 16, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.933 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 21989 / % possible obs: 76.2 % / Redundancy: 4.1 % / Biso Wilson estimate: 35.15 Å2 / Net I/σ(I): 15.2 |
-Processing
Software | Name: BUSTER / Version: 2.10.0 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.01→19.93 Å / Cor.coef. Fo:Fc: 0.9374 / Cor.coef. Fo:Fc free: 0.9096 / SU R Cruickshank DPI: 0.328 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.31 / SU Rfree Blow DPI: 0.22 / SU Rfree Cruickshank DPI: 0.227
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Displacement parameters | Biso mean: 45.56 Å2
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Refine analyze | Luzzati coordinate error obs: 0.276 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 2.01→19.93 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.01→2.11 Å / Total num. of bins used: 11
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