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- PDB-4ol8: Ty3 reverse transcriptase bound to DNA/RNA -

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Basic information

Entry
Database: PDB / ID: 4ol8
TitleTy3 reverse transcriptase bound to DNA/RNA
Components
  • 5'-D(*CP*AP*TP*CP*TP*TP*CP*CP*TP*CP*TP*CP*TP*CP*TP*C)-3'
  • 5'-R(*CP*UP*GP*AP*GP*AP*GP*AP*GP*AP*GP*GP*AP*AP*GP*AP*UP*G)-3'
  • Reverse transcriptase/ribonuclease H
KeywordsTRANSFERASE / HYDROLASE/RNA/DNA / Protein-DNA/RNA / reverse transcription / DNA/RNA binding / HYDROLASE-RNA-DNA complex
Function / homology
Function and homology information


retrotransposon nucleocapsid / retrotransposition / Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / ribonuclease H / RNA nuclease activity / DNA integration / RNA-directed DNA polymerase / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / peptidase activity ...retrotransposon nucleocapsid / retrotransposition / Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / ribonuclease H / RNA nuclease activity / DNA integration / RNA-directed DNA polymerase / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / peptidase activity / DNA recombination / DNA-directed DNA polymerase / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / proteolysis / DNA binding / RNA binding / zinc ion binding / ATP binding / nucleus / cytoplasm
Similarity search - Function
Peptidase A2B, Ty3 transposon peptidase / Ty3 transposon peptidase / Ty3 transposon capsid-like protein / Ty3 transposon capsid-like protein / Integrase zinc-binding domain / Integrase zinc binding domain / Reverse transcriptase/retrotransposon-derived protein, RNase H-like domain / RNase H-like domain found in reverse transcriptase / HIV Type 1 Reverse Transcriptase, subunit A, domain 1 / HIV Type 1 Reverse Transcriptase; Chain A, domain 1 ...Peptidase A2B, Ty3 transposon peptidase / Ty3 transposon peptidase / Ty3 transposon capsid-like protein / Ty3 transposon capsid-like protein / Integrase zinc-binding domain / Integrase zinc binding domain / Reverse transcriptase/retrotransposon-derived protein, RNase H-like domain / RNase H-like domain found in reverse transcriptase / HIV Type 1 Reverse Transcriptase, subunit A, domain 1 / HIV Type 1 Reverse Transcriptase; Chain A, domain 1 / Reverse transcriptase/Diguanylate cyclase domain / Integrase core domain / Integrase, catalytic core / Integrase catalytic domain profile. / Reverse transcriptase (RNA-dependent DNA polymerase) / Reverse transcriptase domain / Reverse transcriptase (RT) catalytic domain profile. / zinc finger / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Ribonuclease H superfamily / Aspartic peptidase domain superfamily / Ribonuclease H-like superfamily / Reverse transcriptase/Diguanylate cyclase domain / Alpha-Beta Plaits / Roll / DNA/RNA polymerase superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
DNA / DNA (> 10) / RNA / RNA (> 10) / Transposon Ty3-G Gag-Pol polyprotein
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.1 Å
AuthorsNowak, E. / Miller, J.T. / Bona, M.K. / Studnicka, J. / Szczepanowski, R.H. / Jurkowski, J. / Le Grice, S.F.J. / Nowotny, M.
CitationJournal: Nat.Struct.Mol.Biol. / Year: 2014
Title: Ty3 reverse transcriptase complexed with an RNA-DNA hybrid shows structural and functional asymmetry.
Authors: Nowak, E. / Miller, J.T. / Bona, M.K. / Studnicka, J. / Szczepanowski, R.H. / Jurkowski, J. / Le Grice, S.F. / Nowotny, M.
History
DepositionJan 23, 2014Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 5, 2014Provider: repository / Type: Initial release
Revision 1.1Mar 12, 2014Group: Structure summary
Revision 1.2Apr 2, 2014Group: Database references
Revision 1.3Apr 16, 2014Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Reverse transcriptase/ribonuclease H
B: Reverse transcriptase/ribonuclease H
C: 5'-R(*CP*UP*GP*AP*GP*AP*GP*AP*GP*AP*GP*GP*AP*AP*GP*AP*UP*G)-3'
D: 5'-D(*CP*AP*TP*CP*TP*TP*CP*CP*TP*CP*TP*CP*TP*CP*TP*C)-3'
G: 5'-R(*CP*UP*GP*AP*GP*AP*GP*AP*GP*AP*GP*GP*AP*AP*GP*AP*UP*G)-3'
H: 5'-D(*CP*AP*TP*CP*TP*TP*CP*CP*TP*CP*TP*CP*TP*CP*TP*C)-3'
F: Reverse transcriptase/ribonuclease H
E: Reverse transcriptase/ribonuclease H
hetero molecules


Theoretical massNumber of molelcules
Total (without water)241,72910
Polymers241,5378
Non-polymers1922
Water181
1
A: Reverse transcriptase/ribonuclease H
B: Reverse transcriptase/ribonuclease H
C: 5'-R(*CP*UP*GP*AP*GP*AP*GP*AP*GP*AP*GP*GP*AP*AP*GP*AP*UP*G)-3'
D: 5'-D(*CP*AP*TP*CP*TP*TP*CP*CP*TP*CP*TP*CP*TP*CP*TP*C)-3'
hetero molecules


Theoretical massNumber of molelcules
Total (without water)120,9616
Polymers120,7694
Non-polymers1922
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7630 Å2
ΔGint-53 kcal/mol
Surface area43600 Å2
MethodPISA
2
G: 5'-R(*CP*UP*GP*AP*GP*AP*GP*AP*GP*AP*GP*GP*AP*AP*GP*AP*UP*G)-3'
H: 5'-D(*CP*AP*TP*CP*TP*TP*CP*CP*TP*CP*TP*CP*TP*CP*TP*C)-3'
F: Reverse transcriptase/ribonuclease H
E: Reverse transcriptase/ribonuclease H


Theoretical massNumber of molelcules
Total (without water)120,7694
Polymers120,7694
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7100 Å2
ΔGint-33 kcal/mol
Surface area43990 Å2
MethodPISA
Unit cell
Length a, b, c (Å)320.700, 75.062, 108.350
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212

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Components

#1: Protein
Reverse transcriptase/ribonuclease H / Ty3 reverse transcriptase / RT / RT-RH / p55


Mass: 55059.449 Da / Num. of mol.: 4 / Fragment: UNP residues 536-1101 / Mutation: D426N
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Strain: ATCC 204508 / S288c / Gene: TY3B-G, YGRWTy3-1 POL, YGR109W-B, G5984 / Production host: Escherichia coli (E. coli)
References: UniProt: Q99315, RNA-directed DNA polymerase, DNA-directed DNA polymerase, ribonuclease H
#2: RNA chain 5'-R(*CP*UP*GP*AP*GP*AP*GP*AP*GP*AP*GP*GP*AP*AP*GP*AP*UP*G)-3'


Mass: 5938.644 Da / Num. of mol.: 2 / Source method: obtained synthetically
#3: DNA chain 5'-D(*CP*AP*TP*CP*TP*TP*CP*CP*TP*CP*TP*CP*TP*CP*TP*C)-3'


Mass: 4711.053 Da / Num. of mol.: 2 / Source method: obtained synthetically
#4: Chemical ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.63 Å3/Da / Density % sol: 53.26 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8.5
Details: 0.1 M Tris, 0.2 M ammonium sulfate, 17% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.979 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 4, 2013
RadiationMonochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2.999→48.898 Å / Num. all: 53285 / Num. obs: 53285 / % possible obs: 99.6 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 5.1 % / Rmerge(I) obs: 0.102 / Net I/σ(I): 11.9
Reflection shellResolution: 2.999→3.29 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.847 / Mean I/σ(I) obs: 2.1 / % possible all: 98.4

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Processing

Software
NameVersionClassification
XDSdata scaling
AutoSolphasing
PHENIX(phenix.refine: 1.8.2_1309)refinement
XDSdata reduction
RefinementMethod to determine structure: SAD / Resolution: 3.1→48.898 Å / SU ML: 0.51 / σ(F): 1.3 / Phase error: 30.55 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2956 2425 5 %RANDOM
Rwork0.227 ---
obs0.2305 48418 99.08 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.1→48.898 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12734 1360 10 1 14105
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01614634
X-RAY DIFFRACTIONf_angle_d1.06920104
X-RAY DIFFRACTIONf_dihedral_angle_d15.7825398
X-RAY DIFFRACTIONf_chiral_restr0.0662325
X-RAY DIFFRACTIONf_plane_restr0.0052352
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.1-3.13520.39191580.34822914X-RAY DIFFRACTION100
3.1352-3.17210.37051490.33052820X-RAY DIFFRACTION99
3.1721-3.21080.36941560.32322929X-RAY DIFFRACTION100
3.2108-3.25140.37681510.30072900X-RAY DIFFRACTION100
3.2514-3.29420.35891550.29282901X-RAY DIFFRACTION100
3.2942-3.33930.36461520.28582826X-RAY DIFFRACTION100
3.3393-3.3870.34441580.28152948X-RAY DIFFRACTION99
3.387-3.43750.32831520.28382855X-RAY DIFFRACTION99
3.4375-3.49120.351490.26362872X-RAY DIFFRACTION99
3.4912-3.54850.33781560.25492895X-RAY DIFFRACTION99
3.5485-3.60960.34131490.25282874X-RAY DIFFRACTION99
3.6096-3.67530.29651480.23332852X-RAY DIFFRACTION99
3.6753-3.74590.29791550.22922909X-RAY DIFFRACTION99
3.7459-3.82240.30691510.222834X-RAY DIFFRACTION99
3.8224-3.90540.26991510.21722892X-RAY DIFFRACTION98
3.9054-3.99620.32481530.2252881X-RAY DIFFRACTION99
3.9962-4.09610.29231470.21982853X-RAY DIFFRACTION99
4.0961-4.20680.27931520.21112914X-RAY DIFFRACTION99
4.2068-4.33050.29981500.20692873X-RAY DIFFRACTION99
4.3305-4.47020.29321430.20362860X-RAY DIFFRACTION99
4.4702-4.62990.25281510.19432886X-RAY DIFFRACTION99
4.6299-4.81510.30531530.19032854X-RAY DIFFRACTION99
4.8151-5.0340.21491500.17992904X-RAY DIFFRACTION99
5.034-5.29910.28711500.20222888X-RAY DIFFRACTION99
5.2991-5.63070.29571460.2012850X-RAY DIFFRACTION99
5.6307-6.06470.30331490.21292879X-RAY DIFFRACTION99
6.0647-6.67370.29111450.20672874X-RAY DIFFRACTION98
6.6737-7.63620.25431510.20632860X-RAY DIFFRACTION99
7.6362-9.60880.23951590.2092867X-RAY DIFFRACTION99
9.6088-48.9040.30451510.2492828X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.61471.246-0.80861.0236-0.20471.3254-0.1442-1.5577-0.23591.27750.2929-0.0652-0.0439-0.3731-0.13991.57940.4804-0.17321.88640.03060.7656-69.0606-2.378960.1986
22.80761.7024-1.05964.02091.17771.52660.6009-1.535-0.45621.1104-0.3043-0.3411-0.4635-1.6431-0.21841.56860.2776-0.11372.49570.40390.8422-60.8877-12.541168.1716
33.2663-0.69770.50884.1688-0.00921.7179-0.3817-1.4141-0.06070.83790.1803-0.21720.01180.20790.191.10870.3090.00111.75440.04560.6195-64.8311-1.05354.9873
47.18260.16240.06538.53741.49795.8633-0.9428-1.59731.35920.10470.9728-0.4075-0.9659-0.64220.0510.99440.1819-0.23631.3745-0.37020.8212-68.281517.392151.9957
53.5142-0.67940.54126.45572.62294.6427-0.8914-1.0550.52710.52150.7403-0.4997-1.6261-0.33530.0451.3280.48-0.17421.6158-0.50160.7205-73.37817.446660.7714
61.57840.6081-2.60793.4703-0.92164.5221-0.4221-1.69340.9210.538-0.1965-1.54590.12220.99480.42511.27350.1105-0.57841.9259-0.37321.2405-45.612915.453252.6672
70.62380.5149-1.21362.7411-0.48632.4662-0.5241-0.86870.34021.0908-0.4444-1.6389-0.5522.0430.15840.8061-0.4752-0.37311.663-0.40211.3234-37.938418.814543.7955
80.13450.38810.72655.40380.42964.4217-0.7629-0.16431.5611.0305-0.0418-0.072-0.86830.69570.38431.5435-0.3325-0.42771.3323-0.56051.6518-47.866630.557235.6794
98.0972-2.0572-0.22313.6792-1.97671.3353-0.1691-0.53870.46030.52610.0447-1.7727-0.24820.93260.01491.0073-0.1933-0.20850.9689-0.18431.3188-44.700119.254429.6381
103.1722-2.3506-3.08871.75471.98089.21210.194-0.11690.3185-0.081-0.0118-0.1152-0.53340.3336-0.16981.012-0.2414-0.09041.01-0.06471.2442-53.246723.856325.9657
113.6856-2.50422.1873.69751.90567.0296-0.7552-0.92240.82670.2277-0.1199-0.1277-0.2394-1.44350.82220.76490.02610.00540.8717-0.05230.7501-96.55067.328119.9422
127.37040.0271-0.65725.5097-0.27575.37150.2111-0.68460.26410.4509-0.2878-0.1362-0.40720.12540.10390.68650.0638-0.1050.7996-0.03740.5174-71.69226.902231.1673
137.4605-1.01511.7062.5448-0.72095.28020.07910.5786-0.3453-0.3285-0.06660.35550.6257-0.2699-0.00320.6983-0.0176-0.02350.535-0.06560.5435-88.46971.299513.5678
148.6844.0041-2.90324.1877-2.34222.4698-0.34960.8683-0.4773-0.931-0.1433-0.62170.3091-0.03960.45961.18580.18980.10721.14320.07610.7575-66.908113.824-9.9511
155.70631.8153-1.42925.3609-2.73429.2084-0.16551.26290.064-0.8530.1925-0.21160.39030.3574-0.02410.81930.05390.09580.84740.07090.5954-62.82619.4664-7.4232
167.10751.31870.29022.9767-0.0167.43770.0439-0.1337-0.0114-0.167-0.1487-0.3576-0.3822-0.01530.13390.78840.09440.09510.47120.12590.6156-63.26897.7719.8186
179.40583.2914-0.63933.86313.00464.1174-0.5438-0.7305-0.1211-0.0024-0.4853-1.1126-0.28442.08851.22911.15410.1109-0.13521.81050.08090.7715-56.4571-0.718147.5888
187.36851.96934.07927.61596.72887.6252-0.68180.1306-1.8105-0.39141.442-0.99710.94073.5442-0.48031.43670.08910.28421.98090.04771.4798-44.5813-1.995325.0996
196.8529-0.38461.13236.42534.42393.2767-0.0298-0.27060.24590.07370.934-1.98390.64242.4315-1.12691.15060.15220.08912.12420.08921.5535-47.59330.328434.1717
208.48354.3240.73344.41684.00536.0309-0.78992.37162.63180.19140.00190.696-1.32350.38780.92051.02660.2869-0.08751.29370.56691.9186-32.9745-21.2151-33.3704
215.8461.4692-3.77062.20311.02344.5427-0.2609-1.58552.73390.76710.2059-1.14160.6130.9650.02441.43060.5391-0.0641.6746-0.07032.1329-39.1215-8.6863-11.3069
226.5923-5.4726-4.74954.53483.92393.76981.3216-0.26492.68510.6684-2.17112.0691-3.83740.44320.66111.9505-0.0540.28351.4941-0.48512.1493-36.105-6.2517-1.2544
235.52494.5394-7.05866.8896-4.73379.3850.36071.10331.8841-0.75792.0408-0.22640.9552-0.8533-2.28891.17510.3664-0.01441.61260.38281.8485-41.717-14.5278-17.4571
240.61280.01070.23620.1446-0.02330.0993-0.75410.03220.9408-0.26-0.2601-1.11940.10370.89470.74341.24210.52520.13861.49450.95672.0813-32.0195-10.1453-34.1456
253.47523.8958-0.47656.8171-2.77797.47490.31420.5436-0.0332-0.2527-0.3031-0.52020.33410.72710.012.0841.09-0.6273.330.60992.596-24.9496-20.6296-40.4019
263.7774-2.03121.74423.8759-2.32414.4799-0.06-0.545-0.40680.02830.3560.12651.03830.1079-0.29881.17980.2096-0.17680.62940.05361.1885-22.4222-45.7553-3.1673
270.44650.28170.05270.27380.06180.35920.23950.06120.193-0.1862-0.8873-0.0105-0.8892-0.31610.63492.01660.5292-0.38111.2902-0.24131.154-25.5262-23.74229.9851
282.77210.5850.26213.7538-1.75678.4426-0.1907-1.05091.12961.10340.51340.0136-1.0551-0.6352-0.29191.25180.4111-0.22721.0741-0.43041.3539-27.716-26.05079.3097
292.0614-0.40421.09081.14940.30641.03611.37011.9568-0.8548-0.7151-0.7431-0.23870.65650.8083-0.64661.45690.6758-0.01612.18760.27651.5814-29.4924-34.5493-45.5405
302.6018-1.73992.04531.1118-1.37586.89521.27611.74190.1959-1.1818-0.4686-0.23050.6912-0.5773-0.94851.55650.6964-0.07361.94760.30181.3578-38.9174-28.6214-47.0044
310.8756-0.7567-1.09991.727-0.252.31340.8571.80520.9616-1.04-0.8345-0.41360.17690.23860.0281.58280.97210.16522.09760.46871.5664-18.9687-28.7539-40.6979
328.3096-0.94190.8787.53982.2543.89241.06882.06740.8547-1.3296-0.65911.4-0.662-0.7934-0.41481.24120.17930.0841.5120.49451.9719-25.053-2.1809-38.6265
333.0098-2.1262-1.19812.98552.23052.53410.10370.20810.2244-0.42990.2410.176-1.1797-0.7157-0.11111.76470.3097-0.38730.49820.80872.4526-23.69273.56-29.1355
340.5427-1.68070.48035.6928-0.16836.22510.10520.43642.0808-1.17420.9754-0.3611-2.12460.87890.05491.6167-0.18230.21590.87570.30071.9996-12.0481-2.5679-28.6185
355.92051.1174-4.12651.18240.60835.40891.18910.47240.24090.3407-0.8852-0.5253-1.21010.0201-0.23031.20120.0612-0.04950.83450.14592.1919-15.7775-6.3843-11.9507
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 22 through 70 )
2X-RAY DIFFRACTION2chain 'A' and (resid 71 through 100 )
3X-RAY DIFFRACTION3chain 'A' and (resid 101 through 201 )
4X-RAY DIFFRACTION4chain 'A' and (resid 202 through 221 )
5X-RAY DIFFRACTION5chain 'A' and (resid 222 through 248 )
6X-RAY DIFFRACTION6chain 'A' and (resid 249 through 305 )
7X-RAY DIFFRACTION7chain 'A' and (resid 306 through 352 )
8X-RAY DIFFRACTION8chain 'A' and (resid 353 through 378 )
9X-RAY DIFFRACTION9chain 'A' and (resid 379 through 411 )
10X-RAY DIFFRACTION10chain 'A' and (resid 412 through 459 )
11X-RAY DIFFRACTION11chain 'B' and (resid 15 through 53 )
12X-RAY DIFFRACTION12chain 'B' and (resid 54 through 123 )
13X-RAY DIFFRACTION13chain 'B' and (resid 124 through 262 )
14X-RAY DIFFRACTION14chain 'B' and (resid 263 through 299 )
15X-RAY DIFFRACTION15chain 'B' and (resid 300 through 411 )
16X-RAY DIFFRACTION16chain 'B' and (resid 412 through 476 )
17X-RAY DIFFRACTION17chain 'C' and (resid 2 through 6 )
18X-RAY DIFFRACTION18chain 'C' and (resid 7 through 18 )
19X-RAY DIFFRACTION19chain 'D' and (resid 33 through 47 )
20X-RAY DIFFRACTION20chain 'G' and (resid 2 through 6 )
21X-RAY DIFFRACTION21chain 'G' and (resid 7 through 18 )
22X-RAY DIFFRACTION22chain 'H' and (resid 32 through 36 )
23X-RAY DIFFRACTION23chain 'H' and (resid 37 through 41 )
24X-RAY DIFFRACTION24chain 'H' and (resid 42 through 46 )
25X-RAY DIFFRACTION25chain 'H' and (resid 47 through 47 )
26X-RAY DIFFRACTION26chain 'F' and (resid 15 through 263 )
27X-RAY DIFFRACTION27chain 'F' and (resid 264 through 339 )
28X-RAY DIFFRACTION28chain 'F' and (resid 340 through 473 )
29X-RAY DIFFRACTION29chain 'E' and (resid 22 through 70 )
30X-RAY DIFFRACTION30chain 'E' and (resid 71 through 133 )
31X-RAY DIFFRACTION31chain 'E' and (resid 134 through 262 )
32X-RAY DIFFRACTION32chain 'E' and (resid 263 through 297 )
33X-RAY DIFFRACTION33chain 'E' and (resid 298 through 330 )
34X-RAY DIFFRACTION34chain 'E' and (resid 331 through 358 )
35X-RAY DIFFRACTION35chain 'E' and (resid 359 through 458 )

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