SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 18-STRANDED BARREL THIS IS REPRESENTED BY A 19-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 18.015 Da / Num. of mol.: 242 / Source method: isolated from a natural source / Formula: H2O
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Details
Nonpolymer details
3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL (PE5): PEG 400 LAURYL DIMETHYLAMINE-N-OXIDE (LDA): SOME ...3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL (PE5): PEG 400 LAURYL DIMETHYLAMINE-N-OXIDE (LDA): SOME LDA MOLECULES HAVE NOT HAD HEAD GROUPS FITTED AS NO ELECTRON DENSITY, ALKANE CHAINS WERE MODELLED WHERE WE WERE UNABLE TO DISTINGUISH BETWEEN LADO AND 7.8 MAG. INCLUSION OF THE TAILS FIT INTO DENSITY AND REDUCES FREE R FACTOR BY 1.2 PERCENT.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.36 Å3/Da / Density % sol: 47.96 % / Description: NONE
Crystal grow
Method: lipidic cubic phase / pH: 7.5 Details: 0.1 M TRIS-HCL, PH 7.5, 0.1 M SODIUM CITRATE, 18 %(W/V) PEG 400. PROTEIN WAS MIXED WITH 7.8 MAG AT RATIO 1:1 (W:W) TO FORM CUBIC PHASE.
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