+Open data
-Basic information
Entry | Database: PDB / ID: 3m0d | ||||||
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Title | Crystal structure of the TRAF1:TRAF2:cIAP2 complex | ||||||
Components |
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Keywords | SIGNALING PROTEIN / trimeric helix coiled coiled / acetylation / alternative splicing / apoptosis / coiled coil / cytoplasm / metal-binding / ubl conjugation / polymorphism / chromosomal rearrangement | ||||||
Function / homology | Function and homology information CD40 receptor binding / regulation of extrinsic apoptotic signaling pathway / tumor necrosis factor receptor superfamily complex / regulation of cysteine-type endopeptidase activity / sphingolipid binding / regulation of RIG-I signaling pathway / Defective RIPK1-mediated regulated necrosis / IRE1-TRAF2-ASK1 complex / TRAF2-GSTP1 complex / negative regulation of glial cell apoptotic process ...CD40 receptor binding / regulation of extrinsic apoptotic signaling pathway / tumor necrosis factor receptor superfamily complex / regulation of cysteine-type endopeptidase activity / sphingolipid binding / regulation of RIG-I signaling pathway / Defective RIPK1-mediated regulated necrosis / IRE1-TRAF2-ASK1 complex / TRAF2-GSTP1 complex / negative regulation of glial cell apoptotic process / tumor necrosis factor binding / Regulation by c-FLIP / CASP8 activity is inhibited / Dimerization of procaspase-8 / TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway / interleukin-17-mediated signaling pathway / programmed necrotic cell death / TNF signaling / regulation of necroptotic process / Caspase activation via Death Receptors in the presence of ligand / regulation of nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway / CD40 receptor complex / signal transduction involved in regulation of gene expression / activation of NF-kappaB-inducing kinase activity / vesicle membrane / mRNA stabilization / negative regulation of necroptotic process / positive regulation of tumor necrosis factor-mediated signaling pathway / mitogen-activated protein kinase kinase kinase binding / thioesterase binding / tumor necrosis factor receptor binding / regulation of immunoglobulin production / positive regulation of extrinsic apoptotic signaling pathway / regulation of canonical NF-kappaB signal transduction / TNFR1-induced proapoptotic signaling / RIPK1-mediated regulated necrosis / TRAF6 mediated IRF7 activation / regulation of innate immune response / regulation of toll-like receptor signaling pathway / non-canonical NF-kappaB signal transduction / protein K63-linked ubiquitination / TRAF6 mediated NF-kB activation / cysteine-type endopeptidase inhibitor activity involved in apoptotic process / regulation of JNK cascade / regulation of protein-containing complex assembly / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / cellular response to nitric oxide / protein autoubiquitination / canonical NF-kappaB signal transduction / ubiquitin ligase complex / signaling adaptor activity / positive regulation of JUN kinase activity / tumor necrosis factor-mediated signaling pathway / positive regulation of interleukin-2 production / response to endoplasmic reticulum stress / TICAM1, RIP1-mediated IKK complex recruitment / IKK complex recruitment mediated by RIP1 / TNFR1-induced NF-kappa-B signaling pathway / positive regulation of protein ubiquitination / Regulation of NF-kappa B signaling / TNFR2 non-canonical NF-kB pathway / Regulation of TNFR1 signaling / protein catabolic process / RING-type E3 ubiquitin transferase / NOD1/2 Signaling Pathway / cytoplasmic side of plasma membrane / Regulation of necroptotic cell death / positive regulation of T cell cytokine production / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / positive regulation of DNA-binding transcription factor activity / protein-macromolecule adaptor activity / positive regulation of NF-kappaB transcription factor activity / cell cortex / regulation of inflammatory response / transferase activity / spermatogenesis / protein-containing complex assembly / protein phosphatase binding / positive regulation of canonical NF-kappaB signal transduction / regulation of apoptotic process / cell surface receptor signaling pathway / molecular adaptor activity / regulation of cell cycle / Ub-specific processing proteases / membrane raft / innate immune response / apoptotic process / ubiquitin protein ligase binding / protein-containing complex binding / negative regulation of apoptotic process / protein kinase binding / enzyme binding / signal transduction / protein-containing complex / zinc ion binding / nucleoplasm / identical protein binding / metal ion binding / nucleus Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Kabaleeswaran, V. / Wu, H. | ||||||
Citation | Journal: Mol.Cell / Year: 2010 Title: Crystal structures of the TRAF2: cIAP2 and the TRAF1: TRAF2: cIAP2 complexes: affinity, specificity, and regulation. Authors: Zheng, C. / Kabaleeswaran, V. / Wang, Y. / Cheng, G. / Wu, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3m0d.cif.gz | 117.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3m0d.ent.gz | 91.8 KB | Display | PDB format |
PDBx/mmJSON format | 3m0d.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m0/3m0d ftp://data.pdbj.org/pub/pdb/validation_reports/m0/3m0d | HTTPS FTP |
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-Related structure data
Related structure data | 3m06C 3m0aSC C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 7572.612 Da / Num. of mol.: 2 / Fragment: Residues 266-329 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TRAF2 / Plasmid: pET26B / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)RIPL / References: UniProt: Q12933 #2: Protein | | Mass: 7468.690 Da / Num. of mol.: 1 / Fragment: Residues 266-329 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TRAF1, EBI6 / Production host: Escherichia coli (E. coli) / References: UniProt: Q13077 #3: Protein | | Mass: 8581.972 Da / Num. of mol.: 1 / Fragment: Residues 26-99 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: BIRC3, API2, IAP1, MIHC, RNF49 / Production host: Escherichia coli (E. coli) / References: UniProt: Q13489 #4: Chemical | ChemComp-ZN / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 58.99 % / Mosaicity: 0.486 ° |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 0.15M AmSO4, 0.1M MES, 15% PEG 4000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 20, 2009 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.8→50 Å / Num. obs: 9137 / % possible obs: 99.8 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 2 / Redundancy: 6.1 % / Rmerge(I) obs: 0.097 / Rsym value: 0.062 / Χ2: 1.35 / Net I/σ(I): 8.9 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 3M0A (TRAF2:ciap2) Resolution: 2.8→32.5 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.937 / SU B: 27.453 / SU ML: 0.239 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 1.432 / ESU R Free: 0.334 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 52.197 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→32.5 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.869 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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