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Yorodumi- PDB-2ywx: Crystal structure of phosphoribosylaminoimidazole carboxylase cat... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ywx | ||||||
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Title | Crystal structure of phosphoribosylaminoimidazole carboxylase catalytic subunit from Methanocaldococcus jannaschii | ||||||
Components | Phosphoribosylaminoimidazole carboxylase catalytic subunit | ||||||
Keywords | LYASE / Rossmann fold / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information 5-(carboxyamino)imidazole ribonucleotide mutase / 5-(carboxyamino)imidazole ribonucleotide mutase activity / 'de novo' IMP biosynthetic process Similarity search - Function | ||||||
Biological species | Methanocaldococcus jannaschii (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.31 Å | ||||||
Authors | Kanagawa, M. / Baba, S. / Kuramitsu, S. / Yokoyama, S. / Kawai, G. / Sampei, G. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Crystal structure of phosphoribosylaminoimidazole carboxylase catalytic subunit from Methanocaldococcus jannaschii Authors: Kanagawa, M. / Baba, S. / Kuramitsu, S. / Yokoyama, S. / Kawai, G. / Sampei, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ywx.cif.gz | 42.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ywx.ent.gz | 29.5 KB | Display | PDB format |
PDBx/mmJSON format | 2ywx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yw/2ywx ftp://data.pdbj.org/pub/pdb/validation_reports/yw/2ywx | HTTPS FTP |
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-Related structure data
Related structure data | 1o4vS S: Starting model for refinement |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 16974.027 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanocaldococcus jannaschii (archaea) Plasmid: pET-21a / Production host: Escherichia coli (E. coli) References: UniProt: Q58033, phosphoribosylaminoimidazole carboxylase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.66 Å3/Da / Density % sol: 53.7 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.4 Details: 0.1M Natrium Acetate(pH 4.4), 3.2M Natrium Chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL26B1 / Wavelength: 1 Å |
Detector | Type: RIGAKU JUPITER 210 / Detector: CCD / Date: Mar 18, 2007 |
Radiation | Monochromator: Fixed exit Si double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.31→50 Å / Num. obs: 8318 / % possible obs: 99.9 % / Redundancy: 7.3 % / Biso Wilson estimate: 31.9 Å2 / Rmerge(I) obs: 0.075 |
Reflection shell | Resolution: 2.31→2.39 Å / Redundancy: 6.6 % / Rmerge(I) obs: 0.415 / Num. unique all: 1545 / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1O4V Resolution: 2.31→31.9 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 509961.09 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 33.2955 Å2 / ksol: 0.351168 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.31→31.9 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.31→2.45 Å / Rfactor Rfree error: 0.025 / Total num. of bins used: 6
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Xplor file |
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