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Yorodumi- PDB-2liv: PERIPLASMIC BINDING PROTEIN STRUCTURE AND FUNCTION. REFINED X-RAY... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2liv | ||||||
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Title | PERIPLASMIC BINDING PROTEIN STRUCTURE AND FUNCTION. REFINED X-RAY STRUCTURES OF THE LEUCINE/ISOLEUCINE/VALINE-BINDING PROTEIN AND ITS COMPLEX WITH LEUCINE | ||||||
Components | LEUCINE | ||||||
Keywords | PERIPLASMIC BINDING PROTEIN | ||||||
Function / homology | Function and homology information branched-chain amino acid transport / isoleucine transport / valine transport / L-leucine transport / amino acid transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / periplasmic space / membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.4 Å | ||||||
Authors | Sack, J.S. / Saper, M.A. / Quiocho, F.A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1989 Title: Periplasmic binding protein structure and function. Refined X-ray structures of the leucine/isoleucine/valine-binding protein and its complex with leucine. Authors: Sack, J.S. / Saper, M.A. / Quiocho, F.A. #1: Journal: J.Mol.Biol. / Year: 1989 Title: Structure of the L-Leucine-Binding Protein Refined at 2.4 Angstroms Resolution and Comparison with the Leu(Slash)Ile(Slash)Val-Binding Protein Structure Authors: Sack, J.S. / Trakhanov, S.D. / Tsigannik, I.H. / Quiocho, F.A. #2: Journal: J.Biol.Chem. / Year: 1983 Title: Leucine, Isoleucine, Valine-Binding Protein from Escherichia Coli Authors: Saper, M.A. / Quiocho, F.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2liv.cif.gz | 77.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2liv.ent.gz | 58.2 KB | Display | PDB format |
PDBx/mmJSON format | 2liv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2liv_validation.pdf.gz | 370.8 KB | Display | wwPDB validaton report |
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Full document | 2liv_full_validation.pdf.gz | 405.5 KB | Display | |
Data in XML | 2liv_validation.xml.gz | 13.1 KB | Display | |
Data in CIF | 2liv_validation.cif.gz | 18.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/li/2liv ftp://data.pdbj.org/pub/pdb/validation_reports/li/2liv | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36784.344 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / References: UniProt: P02917, UniProt: P0AD96*PLUS |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.69 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.6 / Method: other | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.4 Å / Num. obs: 12004 / Num. measured all: 41179 / Rmerge(I) obs: 0.021 |
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-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.4→10 Å /
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Refinement step | Cycle: LAST / Resolution: 2.4→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 10 Å / Num. reflection all: 11817 / Rfactor all: 0.179 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |