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- PDB-2b0u: The Structure of the Follistatin:Activin Complex -

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Basic information

Entry
Database: PDB / ID: 2b0u
TitleThe Structure of the Follistatin:Activin Complex
Components
  • Follistatin
  • Inhibin beta A chain
KeywordsSIGNALING PROTEIN / activin / follistatin / TGF-beta / morphogen / inhibin
Function / homology
Function and homology information


activin receptor antagonist activity / activin A complex / inhibin A complex / cardiac fibroblast cell development / regulation of follicle-stimulating hormone secretion / negative regulation of B cell differentiation / positive regulation of ovulation / GABAergic neuron differentiation / Antagonism of Activin by Follistatin / negative regulation of follicle-stimulating hormone secretion ...activin receptor antagonist activity / activin A complex / inhibin A complex / cardiac fibroblast cell development / regulation of follicle-stimulating hormone secretion / negative regulation of B cell differentiation / positive regulation of ovulation / GABAergic neuron differentiation / Antagonism of Activin by Follistatin / negative regulation of follicle-stimulating hormone secretion / progesterone secretion / type II activin receptor binding / striatal medium spiny neuron differentiation / ameloblast differentiation / negative regulation of macrophage differentiation / Glycoprotein hormones / positive regulation of follicle-stimulating hormone secretion / cellular response to oxygen-glucose deprivation / hemoglobin biosynthetic process / positive regulation of hair follicle development / regulation of BMP signaling pathway / gamete generation / cellular response to follicle-stimulating hormone stimulus / cellular response to cholesterol / pattern specification process / Signaling by BMP / activin binding / negative regulation of phosphorylation / activin receptor signaling pathway / heparan sulfate proteoglycan binding / Signaling by Activin / negative regulation of activin receptor signaling pathway / SMAD protein signal transduction / mesodermal cell differentiation / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / cellular response to angiotensin / positive regulation of transcription by RNA polymerase III / odontogenesis / negative regulation of epithelial cell differentiation / response to aldosterone / hair follicle morphogenesis / negative regulation of G1/S transition of mitotic cell cycle / female gonad development / roof of mouth development / eyelid development in camera-type eye / endodermal cell differentiation / odontogenesis of dentin-containing tooth / peptide hormone binding / positive regulation of SMAD protein signal transduction / negative regulation of type II interferon production / keratinocyte proliferation / hair follicle development / positive regulation of collagen biosynthetic process / BMP signaling pathway / hematopoietic progenitor cell differentiation / extrinsic apoptotic signaling pathway / ovarian follicle development / positive regulation of protein metabolic process / erythrocyte differentiation / positive regulation of erythrocyte differentiation / skeletal system development / cytokine activity / growth factor activity / defense response / hormone activity / negative regulation of cell growth / response to organic cyclic compound / autophagy / cytokine-mediated signaling pathway / male gonad development / cell-cell signaling / nervous system development / cellular response to hypoxia / transcription by RNA polymerase II / cell differentiation / positive regulation of ERK1 and ERK2 cascade / cell surface receptor signaling pathway / positive regulation of protein phosphorylation / negative regulation of cell population proliferation / protein-containing complex binding / positive regulation of gene expression / regulation of transcription by RNA polymerase II / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region / identical protein binding / nucleus / cytoplasm
Similarity search - Function
Follistatin, N-terminal / Extracellular Matrix Fibrillin / TGF-beta binding (TB) domain / Inhibin, beta A subunit / Follistatin/Osteonectin EGF domain / Follistatin/Osteonectin-like EGF domain / TB domain / TGF-beta binding (TB) domain profile. / TGF-beta binding (TB) domain superfamily / Follistatin-like, N-terminal ...Follistatin, N-terminal / Extracellular Matrix Fibrillin / TGF-beta binding (TB) domain / Inhibin, beta A subunit / Follistatin/Osteonectin EGF domain / Follistatin/Osteonectin-like EGF domain / TB domain / TGF-beta binding (TB) domain profile. / TGF-beta binding (TB) domain superfamily / Follistatin-like, N-terminal / Follistatin-N-terminal domain-like / Kazal-type serine protease inhibitor domain / Wheat Germ Agglutinin (Isolectin 2); domain 1 - #30 / TGF-beta, propeptide / TGF-beta propeptide / Transforming growth factor beta, conserved site / TGF-beta family signature. / Transforming growth factor-beta-related / Kazal type serine protease inhibitors / Transforming growth factor-beta (TGF-beta) family / Transforming growth factor-beta, C-terminal / Transforming growth factor beta like domain / TGF-beta family profile. / Kazal domain superfamily / Kazal domain / Kazal domain profile. / Cystine Knot Cytokines, subunit B / Cystine-knot cytokines / Wheat Germ Agglutinin (Isolectin 2); domain 1 / Cystine-knot cytokine / Ribbon / Alpha-Beta Complex / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
IRIDIUM (III) ION / MALONATE ION / Inhibin beta A chain / Follistatin
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MIR / MIRAS / Resolution: 2.8 Å
AuthorsThompson, T.B. / Lerch, T.F. / Cook, R.W. / Woodruff, T.K. / Jardetzky, T.S.
CitationJournal: Dev.Cell / Year: 2005
Title: The Structure of the Follistatin:Activin Complex Reveals Antagonism of Both Type I and Type II Receptor Binding.
Authors: Thompson, T.B. / Lerch, T.F. / Cook, R.W. / Woodruff, T.K. / Jardetzky, T.S.
History
DepositionSep 14, 2005Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 11, 2005Provider: repository / Type: Initial release
Revision 1.1May 1, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Jul 27, 2011Group: Version format compliance
Revision 1.4Oct 11, 2017Group: Refinement description / Category: software / Item: _software.classification / _software.name

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Inhibin beta A chain
B: Inhibin beta A chain
C: Follistatin
D: Follistatin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)90,65614
Polymers89,1684
Non-polymers1,48710
Water2,540141
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area12560 Å2
ΔGint-97 kcal/mol
Surface area40100 Å2
MethodPISA
Unit cell
Length a, b, c (Å)109.250, 120.820, 70.570
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212

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Components

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Protein , 2 types, 4 molecules ABCD

#1: Protein Inhibin beta A chain / Activin beta-A chain / Erythroid differentiation protein / EDF


Mass: 12991.865 Da / Num. of mol.: 2 / Fragment: Activin (mature form)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: INHBA / Cell (production host): Ovary / Production host: Cricetulus griseus (Chinese hamster) / References: UniProt: P08476
#2: Protein Follistatin / / FS / Activin-binding protein


Mass: 31592.205 Da / Num. of mol.: 2 / Fragment: Follistatin-288
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FST / Cell (production host): Ovary / Production host: Cricetulus griseus (Chinese hamster) / References: UniProt: P19883

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Non-polymers , 4 types, 151 molecules

#3: Chemical
ChemComp-IR3 / IRIDIUM (III) ION / Iridium


Mass: 192.217 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Ir
#4: Chemical
ChemComp-MLI / MALONATE ION / Malonic acid


Mass: 102.046 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C3H2O4
#5: Chemical ChemComp-MPD / (4S)-2-METHYL-2,4-PENTANEDIOL / 2-Methyl-2,4-pentanediol


Mass: 118.174 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H14O2 / Comment: precipitant*YM
#6: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 141 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.61 Å3/Da / Density % sol: 52.88 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7
Details: PEG 3350, 200 mM Malonate, pH 7.0, vapor diffusion, hanging drop, temperature 295K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 5ID-B / Wavelength: 0.97923 Å
DetectorType: MARRESEARCH / Detector: CCD / Date: Dec 3, 2004
RadiationProtocol: SINGLE WAVELENGTH / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97923 Å / Relative weight: 1
ReflectionResolution: 2.8→19.35 Å / Num. all: 23605 / Num. obs: 23416 / % possible obs: 99.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0

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Phasing

PhasingMethod: MIRAS
Phasing set
IDCell angle alpha (°)Cell angle beta (°)Cell angle gamma (°)Cell length a (Å)Cell length b (Å)Cell length c (Å)D res high (Å)D res low (Å)
MIRAS909090109.25120.8270.572.819.35
1
Phasing MIRResolution: 2.8→29.66 Å / FOM acentric: 0.224 / FOM centric: 0.199 / Reflection acentric: 20139 / Reflection centric: 2620
Phasing MIR der

Der set-ID: 1 / Resolution: 2.8→29.66 Å

IDR cullis acentricR cullis centricPower acentricPower centricReflection acentricReflection centric
ISO_10000200422564
ISO_20.9830.9670.2680.244106051613
ISO_30.9470.9370.4560.39599481535
ISO_40.9770.9780.4520.457117001737
ISO_50.9910.9790.1120.093128341853
ISO_60.9920.9930.2020.1635467977
ISO_70.970.9480.3450.37473291182
ISO_80.9880.9790.1010.089130491849
ISO_91.1381.0470.2540.2467251129
ANO_11000200140
ANO_20.96900.3860107020
ANO_30.90100.7330100400
ANO_40.8800.7840117970
ANO_50.99500.1440129150
ANO_60.98400.252055630
ANO_70.9800.297071410
ANO_80.99500.1710131410
ANO_90.95200.502068210
Phasing MIR der shell
Highest resolution (Å)Lowest resolution (Å)Der-IDR cullis acentricR cullis centricPower acentricPower centricReflection acentricReflection centric
7.6929.66ISO_10000717243
5.537.69ISO_100001530283
4.545.53ISO_100002013295
3.944.54ISO_100002448326
3.533.94ISO_100002841351
3.233.53ISO_100003198359
2.993.23ISO_100003549360
2.82.99ISO_100003746347
7.6929.66ANO_110006910
5.537.69ANO_1100015300
4.545.53ANO_1100020130
3.944.54ANO_1100024480
3.533.94ANO_1100028410
3.233.53ANO_1100031980
2.993.23ANO_1100035470
2.82.99ANO_1100037460
7.6929.66ISO_20.9270.8970.5140.414717231
5.537.69ISO_20.9770.9860.3670.2651530283
4.545.53ISO_210.9830.2090.1482013295
3.944.54ISO_20.9980.9710.1390.1072448326
3.533.94ISO_20.9750.9570.1270.0992841351
3.233.53ISO_20.9640.9420.1140.0931056127
2.993.23ISO_2000000
2.82.99ISO_2000000
7.6929.66ANO_20.67901.57208140
5.537.69ANO_20.80901.081015300
4.545.53ANO_20.93600.547020130
3.944.54ANO_20.99100.258024480
3.533.94ANO_20.99900.111028410
3.233.53ANO_2100.074010560
2.993.23ANO_2000000
2.82.99ANO_2000000
7.6929.66ISO_30.8380.8540.8850.669717231
5.537.69ISO_30.910.9230.6510.4721530283
4.545.53ISO_30.960.960.3620.2462013295
3.944.54ISO_30.9810.9610.220.1732448326
3.533.94ISO_30.9830.9630.180.1372841351
3.233.53ISO_30.9771.010.1660.13539949
2.993.23ISO_3000000
2.82.99ISO_3000000
7.6929.66ANO_30.42703.10508130
5.537.69ANO_30.57802.156015300
4.545.53ANO_30.83100.978020130
3.944.54ANO_30.97900.423024480
3.533.94ANO_30.99700.175028410
3.233.53ANO_3100.1203950
2.993.23ANO_3000000
2.82.99ANO_3000000
7.6929.66ISO_40.9610.9911.0080.824717231
5.537.69ISO_40.9650.9450.6630.5181530283
4.545.53ISO_40.9890.9710.2680.1992013295
3.944.54ISO_40.9890.9670.1470.1032448326
3.533.94ISO_40.960.9450.1110.0862841351
3.233.53ISO_40.9430.9380.0940.0742151251
2.993.23ISO_4000000
2.82.99ISO_4000000
7.6929.66ANO_40.62801.7908140
5.537.69ANO_40.63401.779015300
4.545.53ANO_40.8500.842020130
3.944.54ANO_40.97500.36024480
3.533.94ANO_40.99800.145028410
3.233.53ANO_4100.078021510
2.993.23ANO_4000000
2.82.99ANO_4000000
7.6929.66ISO_50.9330.9310.1850.14717231
5.537.69ISO_50.980.9750.1560.1071530283
4.545.53ISO_51.0030.9880.10.072013295
3.944.54ISO_51.0040.9810.0770.0582448326
3.533.94ISO_50.9940.970.0750.0552841351
3.233.53ISO_50.9760.9580.0820.0673181355
2.993.23ISO_50.941.0510.0850.08610412
2.82.99ISO_5000000
7.6929.66ANO_50.91500.64608130
5.537.69ANO_50.94200.476015300
4.545.53ANO_50.9800.293020130
3.944.54ANO_50.99700.16024480
3.533.94ANO_50.99900.08028410
3.233.53ANO_5100.046031660
2.993.23ANO_5100.05301040
2.82.99ANO_5000000
7.6929.66ISO_60.9850.9760.3150.23717231
5.537.69ISO_60.9810.9590.2340.1551530283
4.545.53ISO_60.9850.9770.10.0682013295
3.944.54ISO_60.9870.9870.060.0441207168
3.533.94ISO_6000000
3.233.53ISO_6000000
2.993.23ISO_6000000
2.82.99ISO_6000000
7.6929.66ANO_60.89500.66308130
5.537.69ANO_60.9600.392015300
4.545.53ANO_60.99900.119020130
3.944.54ANO_6100.047012070
3.533.94ANO_6000000
3.233.53ANO_6000000
2.993.23ANO_6000000
2.82.99ANO_6000000
7.6929.66ISO_70.8530.840.8270.623717231
5.537.69ISO_70.9350.9270.5890.4291530283
4.545.53ISO_70.9850.9810.2460.172013295
3.944.54ISO_70.9931.0270.0920.0792316294
3.533.94ISO_70.9990.9780.0240.02975379
3.233.53ISO_7000000
2.993.23ISO_7000000
2.82.99ISO_7000000
7.6929.66ANO_70.55702.15808140
5.537.69ANO_70.85500.918015300
4.545.53ANO_70.99100.214020130
3.944.54ANO_70.99900.053022400
3.533.94ANO_7100.02205440
3.233.53ANO_7000000
2.993.23ANO_7000000
2.82.99ANO_7000000
7.6929.66ISO_80.9350.940.1670.135717231
5.537.69ISO_80.9820.9720.150.1131530283
4.545.53ISO_80.9960.9810.0910.0622013294
3.944.54ISO_810.9790.0620.0472448323
3.533.94ISO_80.9870.9660.0560.0432841344
3.233.53ISO_80.9820.9630.0520.0413198351
2.993.23ISO_80.9711.0020.0550.03230223
2.82.99ISO_8000000
7.6929.66ANO_80.93600.56908130
5.537.69ANO_80.94200.553015300
4.545.53ANO_80.98700.322020130
3.944.54ANO_80.99900.161024480
3.533.94ANO_8100.075028410
3.233.53ANO_8100.042031950
2.993.23ANO_8100.03603010
2.82.99ANO_8000000
7.6929.66ISO_91.021.0480.3710.297717231
5.537.69ISO_91.0830.980.3130.2681530283
4.545.53ISO_91.1591.0290.230.2132013295
3.944.54ISO_91.2031.0530.170.1632292293
3.533.94ISO_91.2581.3280.1470.1517327
3.233.53ISO_9000000
2.993.23ISO_9000000
2.82.99ISO_9000000
7.6929.66ANO_90.80101.11708140
5.537.69ANO_90.900.771015300
4.545.53ANO_90.95100.476020130
3.944.54ANO_90.98900.277022910
3.533.94ANO_90.99800.16501730
3.233.53ANO_9000000
2.993.23ANO_9000000
2.82.99ANO_9000000
Phasing MIR der site

Der-ID: ISO_1

IDBiso (Å)Cartn x (Å)Cartn y (Å)Cartn z (Å)Atom type symbolOccupancy
199.2467.76212.73114.485Pt0.39
2269.5588.60335.55233.689Pt0.33
3261.859.90354.2710.964Pt0.47
4263.0832.03646.26617.694Pt0.01
53003.18519.85631.62Pt0.28
6187.3222.49321.02924.419Pt0.16
7299.9572.07529.46811.411Pt0.34
874.2754.79317.85728.891Pt0.07
9111.8567.14916.4911.729Os0.7
10204.868.10951.5630.727Os0.72
11252.4288.0949.43719.168Os0.69
12134.9969.35339.51215.865Os0.37
13114.0437.0634.59542.045Os0.15
14227.0283.61699.425.856Os0.28
15120.6228.81432.45620.865Os0.26
16130.3815.2786.22242.088Os0.23
17205.748.96747.5968.942Ir0.82
18236.5432.48745.44117.3Ir0.58
1965.9266.93815.91211.796Ir0.1
20160.731.662012.554Ir0.39
21248.9954.76013.38Ir0.3
22158.6847.0830.7859.689Ir0.27
23101.4869.57814.14844.868Ir0.14
24224.8518.4872.74361.89Ir0.23
2530016.65812.28767.43Ir0.48
26205.2267.11414.5231.941Ir0.29
27300105.6129.06926.069Ir0.35
2851.756.3522.61929.425Pb0.11
2986.1978.1439.26123.116Pb0.08
30181.5189.32625.2996.693Pb0.14
31260.3586.6848.49819.862Pb0.14
32183.8237.118114.74118.349Pb0.09
33209.3768.27751.3930.398Pb0.13
3460.7948.69548.0088.476Pb0.02
3530099.23115.20431.135Ir0.11
36300103.76813.20661.857Ir0.17
37206.6767.78250.62230.758Ir0.21
383088.08714.12452.977Ir0.01
393033.32746.28617.593Ir0.01
40215.9387.7269.55719.583Ir0.2
41210.1468.21251.19230.827Os0.81
42186.1788.1169.15719.555Os0.72
43239.9667.34316.52212.145Os0.51
443050.14947.7128.2Os0.01
45191.0714.45721.13155.115Os0.22
46276.4825.44121.55426.047Os0.38
47113.8766.98316.33611.618Au0.18
48169.3369.74523.7411.848Au0.1
493076.889-34.54670.624Au0.01
5067.3576.5440.82227.24Au0.05
5186.8948.9666.18615.638Au0.05
5252.0973.5991.74815.528Au0.64
53103.4269.8814.71418.652Au0.95
Phasing MIR shell
Resolution (Å)FOM acentricFOM centricReflection acentricReflection centric
7.69-29.660.950.697814299
5.53-7.690.8390.461530283
4.54-5.530.5810.2622013295
3.94-4.540.3030.1742448326
3.53-3.940.1380.1032841351
3.23-3.530.0440.0373198359
2.99-3.230.0010.0013549360
2.8-2.99003746347

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Processing

Software
NameVersionClassificationNB
SCALAdata scaling
SHARPphasing
CNSrefinement
PDB_EXTRACT1.401data extraction
MAR345data collection
CCP4(SCALA)data scaling
RefinementMethod to determine structure: MIR / Resolution: 2.8→19.35 Å / Data cutoff high absF: 10000 / Data cutoff low absF: 0 / σ(F): 0 / Stereochemistry target values: Engh & Huber
RfactorNum. reflection% reflectionSelection details
Rfree0.297 1177 5 %thin shells
Rwork0.265 ---
all0.301 23381 --
obs0.301 23416 98.9 %-
Displacement parametersBiso mean: 30.103 Å2
Baniso -1Baniso -2Baniso -3
1-3.017 Å20 Å20 Å2
2--1.856 Å20 Å2
3----4.873 Å2
Refinement stepCycle: LAST / Resolution: 2.8→19.35 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5863 0 41 141 6045

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