+Open data
-Basic information
Entry | Database: PDB / ID: 1whi | ||||||
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Title | RIBOSOMAL PROTEIN L14 | ||||||
Components | RIBOSOMAL PROTEIN L14 | ||||||
Keywords | RIBOSOMAL PROTEIN / RRNA-BINDING | ||||||
Function / homology | Function and homology information large ribosomal subunit / rRNA binding / structural constituent of ribosome / translation Similarity search - Function | ||||||
Biological species | Geobacillus stearothermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.5 Å | ||||||
Authors | Davies, C. / White, S.W. / Ramakrishnan, V. | ||||||
Citation | Journal: Structure / Year: 1996 Title: The crystal structure of ribosomal protein L14 reveals an important organizational component of the translational apparatus. Authors: Davies, C. / White, S.W. / Ramakrishnan, V. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1994 Title: Crystallization and Preliminary X-Ray Diffraction of Bacterial Ribosomal Protein L14 Authors: Davies, C. / Gerchman, S.E. / Kycia, J.H. / Mcgee, K. / Ramakrishnan, V. / White, S.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1whi.cif.gz | 36.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1whi.ent.gz | 25.2 KB | Display | PDB format |
PDBx/mmJSON format | 1whi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wh/1whi ftp://data.pdbj.org/pub/pdb/validation_reports/wh/1whi | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13369.613 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Geobacillus stearothermophilus (bacteria) Description: T7 EXPRESSION SYSTEM INDUCIBLE BY ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE (IPTG) Cell line: BL21 / Gene: BACILLUS STEAROTHERMOPHILUS / Plasmid: PET13 Gene (production host): BACILLUS STEAROTHERMOPHILUS (ACCESSION P04450) Production host: Escherichia coli (E. coli) / References: UniProt: P04450 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 38.2 % |
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Crystal grow | pH: 7 / Details: pH 7.0 |
Crystal grow | *PLUS Temperature: 295 K / Method: vapor diffusion, hanging dropDetails: Davies, C., (1994) Acta Crystallogr.,Sect.D, 50, 790. |
Components of the solutions | *PLUS Conc.: 1.0-1.5 M / Common name: sodium citrate |
-Data collection
Diffraction | Mean temperature: 295 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 1 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 12, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→14.6 Å / Num. obs: 15965 / % possible obs: 94.5 % / Observed criterion σ(I): 0 / Redundancy: 2.44 % / Rmerge(I) obs: 0.042 |
-Processing
Software |
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Refinement | Resolution: 1.5→8 Å / σ(F): 0
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Displacement parameters | Biso mean: 13.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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