+Open data
-Basic information
Entry | Database: PDB / ID: 1uuq | ||||||
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Title | Exo-mannosidase from Cellvibrio mixtus | ||||||
Components | MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE | ||||||
Keywords | HYDROLASE / MANNOSIDASE / MANNAN / GLYCOSIDE HYDROLASE / FAMILY 5 | ||||||
Function / homology | Function and homology information | ||||||
Biological species | CELLVIBRIO MIXTUS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 1.5 Å | ||||||
Authors | Dias, M.V.F. / Vincent, F. / Pell, G. / Prates, J.A.M. / Centeno, M.S.J. / Ferreira, L.M.A. / Gilbert, H.J. / Davies, G.J. / Fontes, C.M.G.A. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2004 Title: Insights Into the Molecular Determinants of Substrate Specificity in Glycoside Hydrolase Family 5 Revealed by the Crystal Structure and Kinetics of Cellvibrio Mixtus Mannosidase 5A Authors: Dias, M.V.F. / Vincent, F. / Pell, G. / Prates, J.A.M. / Centeno, M.S.J. / Ferreira, L.M.A. / Gilbert, H.J. / Davies, G.J. / Fontes, C.M.G.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uuq.cif.gz | 108.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uuq.ent.gz | 88.1 KB | Display | PDB format |
PDBx/mmJSON format | 1uuq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uu/1uuq ftp://data.pdbj.org/pub/pdb/validation_reports/uu/1uuq | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 50230.723 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) CELLVIBRIO MIXTUS (bacteria) / Strain: NCIMB 8633 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q6QT42*PLUS | ||||
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#2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.6 % |
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Crystal grow | pH: 4.6 Details: 24% PEG 4000, 0.1M NA ACETATE PH 4.6, 0.2M AMMONIUM SULFATE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.9791 |
Detector | Date: Nov 15, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→40 Å / Num. obs: 73917 / % possible obs: 97.8 % / Redundancy: 6 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 20.8 |
Reflection shell | Resolution: 1.5→1.55 Å / Redundancy: 3.13 % / Rmerge(I) obs: 0.267 / Mean I/σ(I) obs: 3.57 / % possible all: 81.7 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER / Resolution: 1.5→19.88 Å / Cor.coef. Fo:Fc: 0.977 / Cor.coef. Fo:Fc free: 0.964 / SU B: 0.825 / SU ML: 0.031 / Cross valid method: THROUGHOUT / ESU R: 0.066 / ESU R Free: 0.057 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS DISORDERED REGIONS WERE NOT BUILT: THE N- TERMIANL EXTREMITY (RESIDUS 1 - 20) AND THE C-TERMINAL EXTREMITY (RESIDUS 432 - 440)
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.8 Å2
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Refinement step | Cycle: LAST / Resolution: 1.5→19.88 Å
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Refine LS restraints |
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