+Open data
-Basic information
Entry | Database: PDB / ID: 1t50 | |||||||||
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Title | NMR SOLUTION STRUCTURE OF APLYSIA ATTRACTIN | |||||||||
Components | Attractin | |||||||||
Keywords | ATTRACTIN / MOLLUSK / PHEROMONE / APLYSIA ATTRACTIN | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Aplysia californica (California sea hare) | |||||||||
Method | SOLUTION NMR / SELF-CORRECTING DG, VTF | |||||||||
Authors | Ravindranath, G. / Xu, Y. / Schein, C.H. / Rajaratnam, K. / Painter, S.D. / Nagle, G.T. / Braun, W. | |||||||||
Citation | Journal: Biochemistry / Year: 2003 Title: NMR Solution Structure of Attractin, a Water-Borne Pheromone from the Mollusk Aplysia Attractin Authors: Ravindranath, G. / Xu, Y. / Schein, C.H. / Rajarathnam, K. / Nagle, G.T. / Painter, S.D. / Braun, W. #1: Journal: Biophys.J. / Year: 2001 Title: Aplysia Attractin: Biophysical Characterization and Modeling of a Water-Borne Pheromone Authors: Schein, C.H. / Nagle, G.T. / Page, J.S. / Sweedler, J.V. / Xu, Y. / Painter, S.D. / Braun, W. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1t50.cif.gz | 292.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1t50.ent.gz | 253.1 KB | Display | PDB format |
PDBx/mmJSON format | 1t50.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1t50_validation.pdf.gz | 343.5 KB | Display | wwPDB validaton report |
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Full document | 1t50_full_validation.pdf.gz | 461.5 KB | Display | |
Data in XML | 1t50_validation.xml.gz | 17.7 KB | Display | |
Data in CIF | 1t50_validation.cif.gz | 28.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t5/1t50 ftp://data.pdbj.org/pub/pdb/validation_reports/t5/1t50 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6359.892 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aplysia californica (California sea hare) Description: Sf-900 II serum-free medium / Gene: ATT / Cell line (production host): SF9 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: O96910 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: HOMONUCLEAR 1H NMR SPECTROSCOPY |
-Sample preparation
Details | Contents: 0.7mM Unlabled protein, 10 mM sodium phosphate buffer, 90% H20, 10% D20 Solvent system: 90% H20, 10% D20 | |||||||||||||||
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: SELF-CORRECTING DG, VTF / Software ordinal: 1 | ||||||
NMR representative | Selection criteria: closest to the average | ||||||
NMR ensemble | Conformer selection criteria: VTF VALUES / Conformers calculated total number: 50 / Conformers submitted total number: 20 |