+Open data
-Basic information
Entry | Database: PDB / ID: 1swi | ||||||
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Title | GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE | ||||||
Components | GCN4P1 | ||||||
Keywords | LEUCINE ZIPPER / COILED COIL | ||||||
Function / homology | Function and homology information protein localization to nuclear periphery / FCERI mediated MAPK activation / Activation of the AP-1 family of transcription factors / response to amino acid starvation / mediator complex binding / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / nitrogen catabolite activation of transcription from RNA polymerase II promoter / Oxidative Stress Induced Senescence / TFIID-class transcription factor complex binding ...protein localization to nuclear periphery / FCERI mediated MAPK activation / Activation of the AP-1 family of transcription factors / response to amino acid starvation / mediator complex binding / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / nitrogen catabolite activation of transcription from RNA polymerase II promoter / Oxidative Stress Induced Senescence / TFIID-class transcription factor complex binding / positive regulation of RNA polymerase II transcription preinitiation complex assembly / positive regulation of transcription initiation by RNA polymerase II / amino acid biosynthetic process / cellular response to nutrient levels / cellular response to amino acid starvation / RNA polymerase II transcription regulator complex / DNA-binding transcription activator activity, RNA polymerase II-specific / transcription regulator complex / sequence-specific DNA binding / RNA polymerase II-specific DNA-binding transcription factor binding / DNA-binding transcription factor activity, RNA polymerase II-specific / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / chromatin binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.6 Å | ||||||
Authors | Gonzalez, L. / Plecs, J. / Alber, T. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1996 Title: An engineered allosteric switch in leucine-zipper oligomerization. Authors: Gonzalez Jr., L. / Plecs, J.J. / Alber, T. #1: Journal: Nature / Year: 1994 Title: Crystal Structure of an Isoleucine-Zipper Trimer Authors: Harbury, P.B. / Kim, P.S. / Alber, T. #2: Journal: Science / Year: 1993 Title: A Switch between Two-, Three-, and Four-Stranded Coiled Coils in GCN4 Leucine Zipper Mutants Authors: Harbury, P.B. / Zhang, T. / Kim, P.S. / Alber, T. #3: Journal: Science / Year: 1991 Title: X-Ray Structure of the GCN4 Leucine Zipper Authors: O'Shea, E.K. / Klemm, J.D. / Kim, P.S. / Alber, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1swi.cif.gz | 28.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1swi.ent.gz | 19.6 KB | Display | PDB format |
PDBx/mmJSON format | 1swi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1swi_validation.pdf.gz | 391.2 KB | Display | wwPDB validaton report |
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Full document | 1swi_full_validation.pdf.gz | 403.8 KB | Display | |
Data in XML | 1swi_validation.xml.gz | 5.3 KB | Display | |
Data in CIF | 1swi_validation.cif.gz | 6.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sw/1swi ftp://data.pdbj.org/pub/pdb/validation_reports/sw/1swi | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein/peptide | Mass: 3962.639 Da / Num. of mol.: 3 / Mutation: N16A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) References: UniProt: P03069 #2: Chemical | ChemComp-BNZ / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.88 Å3/Da / Density % sol: 34 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Aug 1, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 2618 / % possible obs: 86 % / Observed criterion σ(I): 2 / Redundancy: 2.3 % / Rmerge(I) obs: 0.097 |
Reflection | *PLUS Highest resolution: 2.6 Å |
-Processing
Software |
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Refinement | Resolution: 2.6→6 Å / σ(F): 2 Details: ELECTRON DENSITY FOR THE N-TERMINAL HEPTAD (RESIDUES 1 - 7) IS COMPARATIVELY WEAK, AND THE MAIN CHAIN B VALUES ARE ABOVE AVERAGE. THE N-TERMINAL HEPTAD MAKES RELATIVELY FEW CRYSTAL CONTACTS ...Details: ELECTRON DENSITY FOR THE N-TERMINAL HEPTAD (RESIDUES 1 - 7) IS COMPARATIVELY WEAK, AND THE MAIN CHAIN B VALUES ARE ABOVE AVERAGE. THE N-TERMINAL HEPTAD MAKES RELATIVELY FEW CRYSTAL CONTACTS AND MAY BE DYNAMICALLY DISORDERED.
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Displacement parameters | Biso mean: 44 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→6 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |