+Open data
-Basic information
Entry | Database: PDB / ID: 1sft | ||||||
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Title | ALANINE RACEMASE | ||||||
Components | ALANINE RACEMASE | ||||||
Keywords | ISOMERASE / ALANINE / PYRIDOXAL PHOSPHATE | ||||||
Function / homology | Function and homology information alanine racemase / D-alanine biosynthetic process / alanine racemase activity / pyridoxal phosphate binding Similarity search - Function | ||||||
Biological species | Geobacillus stearothermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Shaw, J.P. / Petsko, G.A. / Ringe, D. | ||||||
Citation | Journal: Biochemistry / Year: 1997 Title: Determination of the structure of alanine racemase from Bacillus stearothermophilus at 1.9-A resolution. Authors: Shaw, J.P. / Petsko, G.A. / Ringe, D. #1: Journal: J.Biol.Chem. / Year: 1987 Title: X-Ray Crystallographic Studies of the Alanine-Specific Racemase from Bacillus Stearothermophilus. Overproduction, Crystallization, and Preliminary Characterization Authors: Neidhart, D.J. / Distefano, M.D. / Tanizawa, K. / Soda, K. / Walsh, C.T. / Petsko, G.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sft.cif.gz | 161.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sft.ent.gz | 133.5 KB | Display | PDB format |
PDBx/mmJSON format | 1sft.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sf/1sft ftp://data.pdbj.org/pub/pdb/validation_reports/sf/1sft | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.8717, 0.1466, -0.4676), Vector: |
-Components
#1: Protein | Mass: 43565.953 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Geobacillus stearothermophilus (bacteria) Strain: IFO 12550 / Gene: ALR / Plasmid: PKK223-3 / Gene (production host): ALR / Production host: Escherichia coli (E. coli) / Strain (production host): XL-1 BLUE / References: UniProt: P10724, alanine racemase #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 45 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 / Details: pH 8.5 | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Nov 26, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→10 Å / Num. obs: 52379 / % possible obs: 83 % / Observed criterion σ(I): 0 / Redundancy: 3.4 % / Rmerge(I) obs: 0.046 / Net I/σ(I): 9.3 |
-Processing
Software |
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Refinement | Resolution: 1.9→10 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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