+Open data
-Basic information
Entry | Database: PDB / ID: 1r36 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | NMR-based structure of autoinhibited murine Ets-1 deltaN301 | ||||||||||||
Components | C-ets-1 protein | ||||||||||||
Keywords | TRANSCRIPTION / transcription factor / autoinhibition module / winged helix-turn-helix / helical bundle | ||||||||||||
Function / homology | Function and homology information Oncogene Induced Senescence / PML body organization / regulation of extracellular matrix disassembly / positive regulation of leukocyte adhesion to vascular endothelial cell / histone acetyltransferase binding / immune system process / negative regulation of cell cycle / positive regulation of blood vessel endothelial cell migration / regulation of angiogenesis / positive regulation of endothelial cell migration ...Oncogene Induced Senescence / PML body organization / regulation of extracellular matrix disassembly / positive regulation of leukocyte adhesion to vascular endothelial cell / histone acetyltransferase binding / immune system process / negative regulation of cell cycle / positive regulation of blood vessel endothelial cell migration / regulation of angiogenesis / positive regulation of endothelial cell migration / transcription corepressor binding / nuclear receptor coactivator activity / positive regulation of erythrocyte differentiation / cell motility / negative regulation of inflammatory response / positive regulation of inflammatory response / positive regulation of miRNA transcription / positive regulation of angiogenesis / sequence-specific double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / regulation of apoptotic process / DNA-binding transcription factor binding / transcription regulator complex / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / transcription by RNA polymerase II / nucleic acid binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / positive regulation of cell migration / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / response to antibiotic / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / identical protein binding / nucleus / cytoplasm Similarity search - Function | ||||||||||||
Biological species | Mus musculus (house mouse) | ||||||||||||
Method | SOLUTION NMR / molecular dynamics simulated annealing | ||||||||||||
Authors | Lee, G.M. / Donaldson, L.W. / Pufall, M.A. / Kang, H.-S. / Pot, I. / Graves, B.J. / McIntosh, L.P. | ||||||||||||
Citation | Journal: J.Biol.Chem. / Year: 2005 Title: The Structural and Dynamic Basis of Ets-1 DNA Binding Autoinhibition Authors: Lee, G.M. / Donaldson, L.W. / Pufall, M.A. / Kang, H.-S. / Pot, I. / Graves, B.J. / McIntosh, L.P. | ||||||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1r36.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1r36.ent.gz | 953.4 KB | Display | PDB format |
PDBx/mmJSON format | 1r36.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r3/1r36 ftp://data.pdbj.org/pub/pdb/validation_reports/r3/1r36 | HTTPS FTP |
---|
-Related structure data
Similar structure data | |
---|---|
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 16252.524 Da / Num. of mol.: 1 / Fragment: Autoinhibition module, residues 301-440 / Mutation: C416S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: pET22b / Production host: Escherichia coli (E. coli) / Strain (production host): HMS174(DE3) / References: UniProt: P27577 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||
NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample conditions | Ionic strength: 500mM NaCl / pH: 6.5 / Pressure: ambient / Temperature: 301 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: molecular dynamics simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 3268 unambiguous and 537 ambiguous NOE-derived distance constraints, 237 dihedral angle restraints, 80 hydrogen bond restraints, and 49 residual ...Details: the structures are based on a total of 3268 unambiguous and 537 ambiguous NOE-derived distance constraints, 237 dihedral angle restraints, 80 hydrogen bond restraints, and 49 residual dipolar coupling restraints. | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 25 |