[English] 日本語
Yorodumi- PDB-1qq0: COBALT SUBSTITUTED CARBONIC ANHYDRASE FROM METHANOSARCINA THERMOPHILA -
+Open data
-Basic information
Entry | Database: PDB / ID: 1qq0 | ||||||
---|---|---|---|---|---|---|---|
Title | COBALT SUBSTITUTED CARBONIC ANHYDRASE FROM METHANOSARCINA THERMOPHILA | ||||||
Components | CARBONIC ANHYDRASE | ||||||
Keywords | LYASE / BETA-HELIX | ||||||
Function / homology | Function and homology information bicarbonate binding / sulfate binding / cobalt ion binding / carbonic anhydrase / carbonate dehydratase activity / zinc ion binding / extracellular region Similarity search - Function | ||||||
Biological species | Methanosarcina thermophila (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.76 Å | ||||||
Authors | Iverson, T.M. / Alber, B.E. / Kisker, C. / Ferry, J.G. / Rees, D.C. | ||||||
Citation | Journal: Biochemistry / Year: 2000 Title: A closer look at the active site of gamma-class carbonic anhydrases: high-resolution crystallographic studies of the carbonic anhydrase from Methanosarcina thermophila. Authors: Iverson, T.M. / Alber, B.E. / Kisker, C. / Ferry, J.G. / Rees, D.C. #1: Journal: Embo J. / Year: 1996 Title: A Left-Handed Beta-Helix Revealed by the Crystal Structure of a Carbonic Anhydrase from the Archaeon Methanosarcina Thermophila Authors: Kisker, C. / Schindelin, H. / Alber, B. / Ferry, J. / Rees, D. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1qq0.cif.gz | 54.7 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1qq0.ent.gz | 38.5 KB | Display | PDB format |
PDBx/mmJSON format | 1qq0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1qq0_validation.pdf.gz | 364.7 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1qq0_full_validation.pdf.gz | 366.7 KB | Display | |
Data in XML | 1qq0_validation.xml.gz | 6 KB | Display | |
Data in CIF | 1qq0_validation.cif.gz | 8.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qq/1qq0 ftp://data.pdbj.org/pub/pdb/validation_reports/qq/1qq0 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 26429.412 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanosarcina thermophila (archaea) / Production host: Escherichia coli (E. coli) / References: UniProt: P40881 |
---|---|
#2: Chemical | ChemComp-CO / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 1.76 Å3/Da / Density % sol: 30.13 % | |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5.8 Details: PEG 8000, AMMONIUM SULFATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 22K | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6.2 | |||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL7-1 / Wavelength: 1.08 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: May 5, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 1.76→19.4 Å / Num. all: 95188 / Num. obs: 18449 / % possible obs: 98.7 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Redundancy: 5.2 % / Biso Wilson estimate: 22 Å2 / Rmerge(I) obs: 0.054 / Net I/σ(I): 21 |
Reflection shell | Resolution: 1.76→1.82 Å / Redundancy: 3 % / Rmerge(I) obs: 0.283 / % possible all: 98.7 |
Reflection | *PLUS Num. measured all: 95188 |
Reflection shell | *PLUS % possible obs: 98.7 % / Mean I/σ(I) obs: 4.2 |
-Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.76→19.4 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: STANDARD CCP4 DICTIONARY
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.76→19.4 Å
| |||||||||||||||||||||||||
Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
|