+Open data
-Basic information
Entry | Database: PDB / ID: 1q67 | ||||||
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Title | Crystal structure of Dcp1p | ||||||
Components | Decapping protein involved in mRNA degradation-Dcp1p | ||||||
Keywords | TRANSCRIPTION / beta sandwich | ||||||
Function / homology | Function and homology information RNA decapping complex / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / deadenylation-independent decapping of nuclear-transcribed mRNA / mRNA decay by 5' to 3' exoribonuclease / cytoplasmic side of membrane / deadenylation-dependent decapping of nuclear-transcribed mRNA / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / enzyme activator activity / P-body ...RNA decapping complex / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / deadenylation-independent decapping of nuclear-transcribed mRNA / mRNA decay by 5' to 3' exoribonuclease / cytoplasmic side of membrane / deadenylation-dependent decapping of nuclear-transcribed mRNA / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / enzyme activator activity / P-body / mRNA processing / mRNA binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.3 Å | ||||||
Authors | She, M. / Decker, C.J. / Liu, Y. / Chen, N. / Parker, R. / Song, H. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2004 Title: Crystal structure of Dcp1p and its functional implications in mRNA decapping Authors: She, M. / Decker, C.J. / Sundramurthy, K. / Liu, Y. / Chen, N. / Parker, R. / Song, H. #1: Journal: Nature / Year: 1996 Title: An essential component of the decapping enzyme required for normal rates of mRNA turnover Authors: Beelman, C.A. / Stevens, A. / Caponigro, G. / LaGrandeur, T.E. / Hatfield, L. / Fortner, D.M. / Parker, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1q67.cif.gz | 79.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1q67.ent.gz | 59.8 KB | Display | PDB format |
PDBx/mmJSON format | 1q67.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/q6/1q67 ftp://data.pdbj.org/pub/pdb/validation_reports/q6/1q67 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 26294.357 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: DCP1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q12517 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.26 Å3/Da / Density % sol: 62.3 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Details: VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.6 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 0.9798 |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9798 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 34251 / % possible obs: 97.9 % / Redundancy: 8.4 % / Rmerge(I) obs: 0.066 / Rsym value: 0.066 / Net I/σ(I): 8.3 |
Reflection shell | Resolution: 2.2→2.26 Å / Redundancy: 4.9 % / Rmerge(I) obs: 0.536 / Mean I/σ(I) obs: 1.1 / Rsym value: 0.536 / % possible all: 97.9 |
Reflection | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 50 Å / Num. measured all: 286716 |
Reflection shell | *PLUS Rmerge(I) obs: 0.371 / Mean I/σ(I) obs: 1.9 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.3→20 Å / Cross valid method: FREE R / Stereochemistry target values: ENGH & HUBER
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Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 20 Å / % reflection Rfree: 10 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.3 |