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Yorodumi- PDB-1osp: CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN A OF BORRELIA BURGDORF... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1osp | ||||||
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Title | CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN A OF BORRELIA BURGDORFERI COMPLEXED WITH A MURINE MONOCLONAL ANTIBODY FAB | ||||||
Components |
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Keywords | COMPLEX (IMMUNOGLOBULIN/LIPOPROTEIN) / COMPLEX (IMMUNOGLOBULIN-LIPOPROTEIN) / OUTER SURFACE PROTEIN A COMPLEXED WITH FAB184.1 / BORRELIA BURGDORFERI STRAIN B31 / COMPLEX (IMMUNOGLOBULIN-LIPOPROTEIN) complex | ||||||
Function / homology | Function and homology information Initial triggering of complement / Classical antibody-mediated complement activation / FCGR activation / Role of phospholipids in phagocytosis / Regulation of Complement cascade / Regulation of actin dynamics for phagocytic cup formation / humoral immune response mediated by circulating immunoglobulin / phagocytosis, recognition / positive regulation of type IIa hypersensitivity / positive regulation of type I hypersensitivity ...Initial triggering of complement / Classical antibody-mediated complement activation / FCGR activation / Role of phospholipids in phagocytosis / Regulation of Complement cascade / Regulation of actin dynamics for phagocytic cup formation / humoral immune response mediated by circulating immunoglobulin / phagocytosis, recognition / positive regulation of type IIa hypersensitivity / positive regulation of type I hypersensitivity / phagocytosis, engulfment / immunoglobulin mediated immune response / immunoglobulin complex, circulating / immunoglobulin receptor binding / positive regulation of phagocytosis / B cell differentiation / complement activation, classical pathway / antigen binding / cell outer membrane / positive regulation of immune response / antibacterial humoral response / cell surface / extracellular space / extracellular region / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Borrelia burgdorferi (Lyme disease spirochete) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.95 Å | ||||||
Authors | Li, H. / Lawson, C.L. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1997 Title: Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab. Authors: Li, H. / Dunn, J.J. / Luft, B.J. / Lawson, C.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1osp.cif.gz | 182.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1osp.ent.gz | 151.5 KB | Display | PDB format |
PDBx/mmJSON format | 1osp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1osp_validation.pdf.gz | 381.5 KB | Display | wwPDB validaton report |
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Full document | 1osp_full_validation.pdf.gz | 391 KB | Display | |
Data in XML | 1osp_validation.xml.gz | 15.2 KB | Display | |
Data in CIF | 1osp_validation.cif.gz | 25.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/os/1osp ftp://data.pdbj.org/pub/pdb/validation_reports/os/1osp | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 23694.061 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: P01837*PLUS |
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#2: Antibody | Mass: 23449.113 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: P01867 |
#3: Protein | Mass: 27682.195 Da / Num. of mol.: 1 / Mutation: CHAIN O, S84C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Borrelia burgdorferi (Lyme disease spirochete) Strain: B31 / Plasmid: PET9C / Production host: Escherichia coli (E. coli) / References: UniProt: P14013, UniProt: P0CL66*PLUS |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 8.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 0.95 / Wavelength: 0.95, 1.1 | |||||||||
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 27, 1995 | |||||||||
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Num. obs: 58870 / % possible obs: 97.1 % / Observed criterion σ(I): 5 / Redundancy: 4.3 % / Rmerge(I) obs: 0.048 | |||||||||
Reflection | *PLUS Highest resolution: 1.95 Å / Num. measured all: 254719 | |||||||||
Reflection shell | *PLUS Highest resolution: 1.95 Å / Lowest resolution: 2.05 Å / % possible obs: 88.3 % / Rmerge(I) obs: 0.216 |
-Processing
Software |
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Refinement | Resolution: 1.95→6 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.95→6 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |